Biologically and environmentally benign approach for PHB-silver nanocomposite synthesis and its characterization

Jayakumar, Anjali; Prabhu, Keerthana; Shah, Lajja; Radha, P.

doi:10.1016/j.polymertesting.2019.106197

Cited by 26 publications

(14 citation statements)

References 42 publications

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“…The inhibitory halo found for our PHB/AgNPs film is higher than the 1.5 mm value reported for the PHB/Ag nanocomposites fabricated recently by Jayakumar et al [ 70 ]. Slepicka et al [ 44 ] demonstrated that their PHB-based films loaded with AgNPs were effective against E. coli microorganisms, but with an inhibitory halo inferior to the control experiment, which is in agreement with our results.…”

Section: Resultscontrasting

confidence: 74%

Exploiting the Physicochemical and Antimicrobial Properties of PHB/PEG and PHB/PEG/ALG-e Blends Loaded with Ag Nanoparticles

et al. 2022

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Poly(3-hydroxybutyrate) (PHB)-based films containing Poly(ethylene glycol) (PEG), esterified sodium alginate (ALG-e) and polymeric additives loaded with Ag nanoparticles (AgNPs) were obtained by a conventional casting method. AgNPs were produced in aqueous suspension and added to polymeric gels using a phase exchange technique. Composite formation was confirmed by finding the Ag peak in the XRD pattern of PHB. The morphological analysis showed that the inclusion of PEG polymer caused the occurrence of pores over the film surface, which were overshadowed by the addition of ALG-e polymer. The PHB functional groups were dominating the FTIR spectrum, whose bands associated with the crystalline and amorphous regions increased after the addition of PEG and ALG-e polymers. Thermal analysis of the films revealed a decrease in the degradation temperature of PHB containing PEG/AgNPs and PEG/ALG-e/AgNPs, suggesting a catalytic effect. The PHB/PEG/ALG-e/AgNPs film combined the best properties of water vapor permeability and hydrophilicity of the different polymers used. All samples showed good antimicrobial activity in vitro, with the greater inhibitory halo observed for the PEG/PEG/AgNPs against Gram positive S. aureus microorganisms. Thus, the PHB/PEG/ALG-e/AgNPs composite demonstrated here is a promising candidate for skin wound healing treatment.

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Section: Resultscontrasting

confidence: 74%

Exploiting the Physicochemical and Antimicrobial Properties of PHB/PEG and PHB/PEG/ALG-e Blends Loaded with Ag Nanoparticles

et al. 2022

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“…The peaks corresponding to the asymmetric C-H stretching vibration of methyl and methylene groups (at 2933 and 2875 cm −1 ) were visible for both samples. Similar peaks have previously been reported for spectra of PHB produced by other organisms, such as P. xenovorans LB400 [36,38]. In the current study, the intensity of these peaks was higher for the PHB produced in the cellulosic hydrolysate than for that produced in the synthetic medium.…”

Section: Fourier Transform Infrared (Ftir) Spectroscopysupporting

confidence: 89%

Production and Characterization of Poly(3-hydroxybutyrate) from Halomonas boliviensis LC1 Cultivated in Hydrolysates of Quinoa Stalks

et al. 2023

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The global production of fossil-based plastics has reached critical levels, and their substitution with bio-based polymers is an urgent requirement. Poly(3-hydroxybutyrate) (PHB) is a biopolymer that can be produced via microbial cultivation, but efficient microorganisms and low-cost substrates are required. Halomonas boliviensis LC1, a moderately halophilic bacterium, is an effective PHB producer, and hydrolysates of the residual stalks of quinoa (Chenopodium quinoa Willd.) can be considered a cheap source of sugars for microbial fermentation processes in quinoa-producing countries. In this study, H. boliviensis LC1 was adapted to a cellulosic hydrolysate of quinoa stalks obtained via acid-catalyzed hydrothermal pretreatment and enzymatic saccharification. The adapted strain was cultivated in hydrolysates and synthetic media, each of them with two different initial concentrations of glucose. Cell growth, glucose consumption, and PHB formation during cultivation were assessed. The cultivation results showed an initial lag in microbial growth and glucose consumption in the quinoa hydrolysates compared to cultivation in synthetic medium, but after 33 h, the values were comparable for all media. Cultivation in hydrolysates with an initial glucose concentration of 15 g/L resulted in a higher glucose consumption rate (0.15 g/(L h) vs. 0.14 g/(L h)) and volumetric productivity of PHB (14.02 mg/(L h) vs. 10.89 mg/(L h)) than cultivation in hydrolysates with 20 g/L as the initial glucose concentration. During most of the cultivation time, the PHB yield on initial glucose was higher for cultivation in synthetic medium than in hydrolysates. The produced PHBs were characterized using advanced analytical techniques, such as high-performance size-exclusion chromatography (HPSEC), Fourier transform infrared (FTIR) spectroscopy, 1H nuclear magnetic resonance (NMR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). HPSEC revealed that the molecular weight of PHB produced in the cellulosic hydrolysate was lower than that of PHB produced in synthetic medium. TGA showed higher thermal stability for PHB produced in synthetic medium than for that produced in the hydrolysate. The results of the other characterization techniques displayed comparable features for both PHB samples. The presented results show the feasibility of producing PHB from quinoa stalks with H. boliviensis.

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“…Despite these good properties, the use of PHB-HV is still quite limited to high-value applications in the medical and pharmaceutical sectors, owing to its relatively high cost (7-12€/kg) [26], compared to traditional no-biodegradable commodity plastics and also to other biopolymers such as poly-lactic acid (PLA) (2.5-3.5€/kg) [27]. For this reason, various studies have been carried out incorporating low-value materials into PHB-HV matrices in order to reduce the cost of the final products, such as starch [28][29][30] and waste lignocellulose fibers, highly-available and at low-cost, sourced from agricultural and industrial crops [20,21,[31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Utilization of coffee silverskin in the production of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) biopolymer-based thermoplastic biocomposites for food contact applications

Gigante

Seggiani

Cinelli

et al. 2021

Composites Part A: Applied Science and Manufacturing

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Biologically and environmentally benign approach for PHB-silver nanocomposite synthesis and its characterization

Cited by 26 publications

References 42 publications

Exploiting the Physicochemical and Antimicrobial Properties of PHB/PEG and PHB/PEG/ALG-e Blends Loaded with Ag Nanoparticles

Exploiting the Physicochemical and Antimicrobial Properties of PHB/PEG and PHB/PEG/ALG-e Blends Loaded with Ag Nanoparticles

Production and Characterization of Poly(3-hydroxybutyrate) from Halomonas boliviensis LC1 Cultivated in Hydrolysates of Quinoa Stalks

Utilization of coffee silverskin in the production of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) biopolymer-based thermoplastic biocomposites for food contact applications

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