Biologically-engineered mechanical model of a calcified artery

Thrivikraman, Greeshma; Johnson, Sharon L.; Syedain, Zeeshan H.; Hill, Ryan C.; Hansen, Kirk C.; Lee, Han Seung; Tranquillo, Robert T.

doi:10.1016/j.actbio.2020.04.018

Cited by 10 publications

(5 citation statements)

References 65 publications

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“…The results indicated that moderate cross-linking of photooxidation and PGG can improve the biomechanical properties of vascular scaffolds, but excessive cross-linking can also lead to reduced vascular compliance. Appropriately cross-linked elastin fibers improved the vascular grafts’ compliance, which has been proven to be related to the intimal hyperplasia in vivo and can reduce the failure rate of vascular graft ( Cocciolone et al, 2018 ; Thrivikraman et al, 2020 ). As a commonly used cross-linking agent, PGG cross-links and stabilizes elastin fiber in vascular graft and also possesses anti-inflammatory, antioxidant, and immunomodulatory effects ( Fraga et al, 2010 ; Leopoldini et al, 2011 ).…”

Section: Discussionmentioning

confidence: 98%

Photooxidation and Pentagalloyl Glucose Cross-Linking Improves the Performance of Decellularized Small-Diameter Vascular Xenograft In Vivo

Liu

Chen

Xie

et al. 2022

Front. Bioeng. Biotechnol.

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Small-diameter vascular grafts have a significant need in peripheral vascular surgery and procedures of coronary artery bypass graft (CABG); however, autografts are not always available, synthetic grafts perform poorly, and allografts and xenografts dilate, calcify, and induce inflammation after implantation. We hypothesized that cross-linking of decellularized xenogeneic vascular grafts would improve the mechanical properties and biocompatibility and reduce inflammation, degradation, and calcification in vivo. To test this hypothesis, the bovine internal mammary artery (BIMA) was decellularized by detergents and ribozymes with sonication and perfusion. Photooxidation and pentagalloyl glucose (PGG) were used to cross-link the collagen and elastin fibers of decellularized xenografts. Modified grafts’ characteristics and biocompatibility were studied in vitro and in vivo; the grafts were implanted as transposition grafts in the subcutaneous of rats and the abdominal aorta of rabbits. The decellularized grafts were cross-linked by photooxidation and PGG, which improved the grafts’ biomechanical properties and biocompatibility, prevented elastic fibers from early degradation, and reduced inflammation and calcification in vivo. Short-term aortic implants in the rabbits showed collagen regeneration and differentiation of host smooth muscle cells. No occlusion and stenosis occurred due to remodeling and stabilization of the neointima. A good patency rate (100%) was maintained. Notably, implantation of non-treated grafts exhibited marked thrombosis, an inflammatory response, calcification, and elastin degeneration. Thus, photooxidation and PGG cross-linking are potential tools for improving grafts’ biological performance within decellularized small-diameter vascular xenografts.

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Section: Discussionmentioning

confidence: 98%

Photooxidation and Pentagalloyl Glucose Cross-Linking Improves the Performance of Decellularized Small-Diameter Vascular Xenograft In Vivo

Liu

Chen

Xie

et al. 2022

Front. Bioeng. Biotechnol.

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“…On the other hand, weakly physisorbed water in the dSCMs was entirely removed at 200°C (Lee et al, 2014). There are some shreds of evidence that collagenous or non-collagenous proteins can be degraded between 250°C and 600°C (Thrivikraman et al, 2020). In addition, mass loss between 600°C and 750°C might have been related to the loss of carbonate resulting from neutralization solution (Galia et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Decellularized spinal cord meninges extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation

Özüdoğru

Isik

Eylem

et al. 2021

J Tissue Eng Regen Med

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Decellularization of extracellular matrices offers an alternative source of regenerative biomaterials that preserve biochemical structure and matrix components of native tissues. In this study, decellularized bovine spinal cord meninges (dSCM)derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges. The success of decellularization is characterized by measuring the contents of residual DNA, glycosaminoglycans, and hydroxyproline, while a proteomics analysis is applied to reveal the composition of MeninGEL. Frequency and temperature sweep rheometry show that dSCM forms self-supporting hydrogel at physiological temperature. The MeninGEL possesses excellent cytocompatibility. Moreover, it is evidenced with immuno/histochemistry and gene expression studies that the hydrogel induces growth-factor free differentiation of human mesenchymal stem cells into neurallineage cells. Furthermore, MeninGEL instructs human umbilical vein endothelial cells to form vascular branching. With its innate bioactivity and low batch-to-batch variation property, the MeninGEL has the potential to be an off-the-shelf product in nerve tissue regeneration and restoration.

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“…[ 14 ] In engineering a model for arterial calcification, collagen hydrogels have been exploited to mimic the chemical and mechanical changes of the healthy tissue through mineralization process. [ 142 ] Farrar et al reported an inexpensive and versatile technique for manufacturing collagen hydrogels to mimic the aortic valve tissue. [ 143 ] This system is compatible with live‐imaging techniques, and each section of the hydrogel can be sectioned and treated for characterization accordingly.…”

Section: In Vitro Models For Pathological Calcificationmentioning

confidence: 99%

Engineered In vitro Models for Pathological Calcification: Routes Toward Mechanistic Understanding

Radvar

Griffanti

Tsolaki

et al. 2021

Advanced NanoBiomed Research

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Physiological calcification plays an essential part in the development of the skeleton and teeth; however, the occurrence of calcification in soft tissues such as the brain, heart, and kidneys associates with health impacts, creating a massive social and economic burden. The current paradigm for pathological calcification focuses on the biological factors responsible for bone‐like mineralization, including osteoblast‐like cells and proteins inducing nucleation and crystal growth. However, the exact mechanism responsible for calcification remains unknown. Toward this goal, this review dissects the current understanding of structure–function relationships and physico‐chemical properties of pathologic calcification from a materials science point of view. We will discuss a range of potential mechanisms of pathological calcification, with the purpose of identifying universal mechanistic pathways that occur across multiple organs/tissues at multiple length scales. The possible effect of extracellular components in signaling and templating mineralization, as well as the role of intrinsically disordered proteins in calcification, is reviewed. The state‐of‐the‐art in vitro models and strategies that can recreate the highly dynamic environment of calcification are identified.

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Biologically-engineered mechanical model of a calcified artery

Cited by 10 publications

References 65 publications

Photooxidation and Pentagalloyl Glucose Cross-Linking Improves the Performance of Decellularized Small-Diameter Vascular Xenograft In Vivo

Photooxidation and Pentagalloyl Glucose Cross-Linking Improves the Performance of Decellularized Small-Diameter Vascular Xenograft In Vivo

Decellularized spinal cord meninges extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation

Engineered In vitro Models for Pathological Calcification: Routes Toward Mechanistic Understanding

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