Biology and applications of endophytic insect-pathogenic fungi

Branine, Margaret; Bazzicalupo, Anna; Branco, Sara

doi:10.1371/journal.ppat.1007831

Cited by 67 publications

(46 citation statements)

References 49 publications

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“…Considering alternative ways of protecting EPF from adverse conditions, the incorporation of EPF in plants as endophytes would appear to be a highly interesting and promising approach (Vega 2018;Branine et al 2019). Furthermore, some insect pests have evolved feeding habits which make them more difficult to control by conventional methods.…”

Section: Introductionmentioning

confidence: 99%

Endophytic colonization of tomato plants by the entomopathogenic fungus Beauveria bassiana for controlling the South American tomato pinworm, Tuta absoluta

Silva

Abib

et al. 2020

CABI Agric Biosci

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Background: The deployment of entomopathogenic fungi (EPF) for the control of crop pests is an important alternative to synthetic pesticides. Despite recent advances in EPF formulations and application techniques, their efficacy is still limited by abiotic and biotic factors. Entomopathogenic fungi naturally colonize plant tissues or they can be artificially inoculated, protecting the plants from insect attack. Beauveria bassiana is the most widely used fungal biological control agent and has potential as an endophyte to protect a range of crops. Although B. bassiana is known to be pathogenic to the South American tomato pinworm, Tuta absoluta (Meyrick), this fungus could be more efficient when deployed as an endophyte to protect tomato plants from attack. Methods: Three B. bassiana isolates were screened for virulence against T. absoluta larvae by application of conidia to leaf surfaces. Following confirmation of virulence, tomato plants were then exposed to conidial suspensions using a forced uptake technique. Only one isolate, LPP139, colonized the leaves. Seedlings were then inoculated with LPP139 and the presence of the fungus in different plant tissues was monitored over 30 days. Possible effects of B. bassiana endophytic colonization on plant development were also evaluated. Following confirmation of endophytic colonization of leaf tissues, T. absoluta 2nd or 3rd instar larvae were offered leaves and survival was monitored over a 7-day period. Results: All three B. bassiana isolates were virulent to T. absoluta, with approximately 90% mortality over 10 days when conidia had been applied to leaf surfaces. Various plant inoculation techniques were tested but only seedling inoculation was successful. LPP139 successfully colonized all of the plant tissues. High percentages of colonization were observed in roots, stems and leaves up to 30 days after inoculation, with no negative effects on plant growth. When T. absoluta larvae were exposed to B. bassiana colonized leaves, survival was reduced to zero over a 7-day period. Conclusions: The endophytic colonization of tomato plants with EPF is a promising method of controlling the South American tomato pinworm. The fungus was detectable for up to 30 days, longer than has been previously observed

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Section: Introductionmentioning

confidence: 99%

Endophytic colonization of tomato plants by the entomopathogenic fungus Beauveria bassiana for controlling the South American tomato pinworm, Tuta absoluta

Silva

Abib

et al. 2020

CABI Agric Biosci

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“…Similar results have been reported on other host plants such as French bean and Faba bean 23,32 . Additionally, perhaps not all insect-pathogenic fungi have the ability to establish themselves as endophytes in living plant tissues 49 . However, numerous studies have documented the ability of Metarhizium spp.…”

Section: Discussionmentioning

confidence: 99%

Endophytic fungi protect tomato and nightshade plants against Tuta absoluta (Lepidoptera: Gelechiidae) through a hidden friendship and cryptic battle

Agbessenou

Akutse

Yusuf

et al. 2020

Sci Rep

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Endophytic fungi live within plant tissues without causing any harm to the host, promote its growth, and induce systemic resistance against pests and diseases. To mitigate the challenging concealed feeding behavior of immature stages of Tuta absoluta in both tomato (Solanum lycopersicum) and nightshade (Solanum scabrum) host plants, 15 fungal isolates were assessed for their endophytic and insecticidal properties. Twelve isolates were endophytic to both host plants with varied colonization rates. Host plants endophytically-colonized by Trichoderma asperellum M2RT4, Beauveria bassiana ICIPE 706 and Hypocrea lixii F3ST1 outperformed all the other isolates in reducing significantly the number of eggs laid, mines developed, pupae formed and adults emerged. Furthermore, the survival of exposed adults and F1 progeny was significantly reduced by Trichoderma sp. F2L41 and B. bassiana isolates ICIPE 35(4) and ICIPE 35(15) compared to other isolates. The results indicate that T. asperellum M2RT4, B. bassiana ICIPE 706 and H. lixii F3ST1 have high potential to be developed as endophytic-fungal-based biopesticide for the management of T. absoluta.

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“…Entomopathogenic fungi from genera Metarhizium and Baeuveria may engage in mutualistic interactions with plants and suppress the infections caused by fungal and viral phytopathogens ( Moonjely, Barelli & Bidochka, 2016 ; Jaber & Enkerli, 2017 ; Jaber & Ownley, 2018 ; Vega, 2018 ; Bamisile et al, 2018 ; Branine, Bazzicalupo & Branco, 2019 ). The suppression of phytopathogenic fungi after the treatment of plants with different species of Beauveria and Metarhizium fungi has been shown in the following systems: Pythium myriotylum and Rhizoctonia solani —tomato and cotton plants ( Ownley et al, 2008 ), Fusarium oxysporum —onion ( Flori & Roberti, 1993 ), powdery mildew—cucumber ( Kim, Goettel & Gillespie, 2010 ), yellow mosaic virus—pumpkin ( Jaber & Salem, 2014 ), and downy mildew—grape ( Jaber, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Entomopathogenic fungi decreaseRhizoctoniadisease in potato in field conditions

Tomilova

Shaldyaeva

Kryukova

et al. 2020

PeerJ

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Rhizoctonia potato disease is widespread in the world and causes substantial yield and quality losses in potato. This study aimed to evaluate the efficacy of entomopathogenic fungi Metarhizium robertsii and Beauveria bassiana in the inhibition of potato Rhizoctonia complex disease. The efficacy of the entomopathogenic fungi M. robertsii and B. bassiana in the defense of potato against Rhizoctonia disease (stem cancer, black scrulf and other forms of manifestation on tubers) was estimated under field conditions in Western Siberia. Preplanting treatment of the tubers with B. bassiana decreased Rhizoctonia disease in the stems and stolons. At the same time, treatment with M. robertsii did not cause a decrease in Rhizoctonia disease in these organs. However, both fungi decreased the sclerotium index on the tubers of new crops. We demonstrated two mechanisms of inhibition of Rhizoctonia solani by M. robertsii and B. bassiana, including (1) direct effect, expressed as inhibition of R. solani sclerotium formation in cocultivation assays, and (2) indirect effect, which is associated with increased peroxidase activity in potato roots under the influence of colonization by entomopathogenic fungi. We suggest that the treatment of seed tubers with B. basiana can effectively manage Rhizoctonia disease during the plant vegetative season and that both fungi significantly improve the quality of the new tuber crop.

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Biology and applications of endophytic insect-pathogenic fungi

Cited by 67 publications

References 49 publications

Endophytic colonization of tomato plants by the entomopathogenic fungus Beauveria bassiana for controlling the South American tomato pinworm, Tuta absoluta

Endophytic colonization of tomato plants by the entomopathogenic fungus Beauveria bassiana for controlling the South American tomato pinworm, Tuta absoluta

Endophytic fungi protect tomato and nightshade plants against Tuta absoluta (Lepidoptera: Gelechiidae) through a hidden friendship and cryptic battle

Entomopathogenic fungi decreaseRhizoctoniadisease in potato in field conditions

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