Biology and Applications of Halophilic and Haloalkaliphilic Actinobacteria

Gohel, Sangeeta D.; Sharma, Amit; Dangar, Kruti G.; Thakrar, Foram J.; Singh, Satya Prakash

doi:10.1201/9781315154695-6

Cited by 4 publications

(1 citation statement)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[1,3,4]. Over the last three decades, there has been increased interest in exploring haloalkaliphiles as a precious resource that produces stable unique exo-enzymes and organic compounds with potential applications in various industrial processes [1,5,6]. However, to date, our knowledge of anaerobic haloalkaliphiles associated with exploitable enzymology and genetic adaptations is still limited.…”

Section: Introductionmentioning

confidence: 99%

Insights into Xylan Degradation and Haloalkaline Adaptation through Whole-Genome Analysis of Alkalitalea saponilacus, an Anaerobic Haloalkaliphilic Bacterium Capable of Secreting Novel Halostable Xylanase

Liao

Holtzapple

Yan

et al. 2018

Genes

View full text Add to dashboard Cite

The obligately anaerobic haloalkaliphilic bacterium Alkalitalea saponilacus can use xylan as the sole carbon source and produce propionate as the main fermentation product. Using mixed carbon sources of 0.4% (w/v) sucrose and 0.1% (w/v) birch xylan, xylanase production from A. saponilacus was 3.2-fold greater than that of individual carbon sources of 0.5% (w/v) sucrose or 0.5% (w/v) birch xylan. The xylanse is halostable and exhibits optimal activity over a broad salt concentration (2–6% NaCl). Its activity increased approximately 1.16-fold by adding 0.2% (v/v) Tween 20. To understand the potential genetic mechanisms of xylan degradation and molecular adaptation to saline-alkali extremes, the complete genome sequence of A. saponilacus was performed with the pacBio single-molecule real-time (SMRT) and Illumina Misseq platforms. The genome contained one chromosome with a total size of 4,775,573 bps, and a G+C genomic content of 39.27%. Ten genes relating to the pathway for complete xylan degradation were systematically identified. Furthermore, various genes were predicted to be involved in isosmotic cytoplasm via the “compatible-solutes strategy” and cytoplasmic pH homeostasis though the “influx of hydrogen ions”. The halostable xylanase from A. saponilacus and its genomic sequence information provide some insight for potential applications in industry under double extreme conditions.

show abstract