Biology and control of<i>Agrobacterium tumefaciens</i>

Schroth, M. N.; Weinhold, A. R.; McCain, A. H.; Hildebrand, D. C.; Ross, Nathan T.

doi:10.3733/hilg.v40n15p537

Cited by 47 publications

(28 citation statements)

References 11 publications

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“…The low density of biovar 2 in the La-Côte-Saint-André bulk soil is an interesting ecological trait of this taxon and is probably related to a property of the local La-Côte-Saint-André environment. The density of biovar 1 agrobacteria reported in the present study, 10 3 to 10 4 CFU ⅐ g (dry weight) of soil Ϫ1 , is similar to the values reported for other soils (22,36). This value is lower than the 10 7 agrobacterium-like colonies per g of soil reported by Bouzar et al (4) under favorable conditions.…”

contrasting

confidence: 49%

Novel Tellurite-Amended Media and Specific Chromosomal and Ti Plasmid Probes for Direct Analysis of Soil Populations of Agrobacterium Biovars 1 and 2

Mougel

Cournoyer

Nesme

2001

Appl Environ Microbiol

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Ecology and biodiversity studies of Agrobacterium spp. require tools such as selective media and DNA probes. Tellurite was tested as a selective agent and a supplement of previously described media for agrobacteria. The known biodiversity within the genus was taken into account when the selectivity of K 2 TeO 3 was analyzed and its potential for isolating Agrobacterium spp. directly from soil was evaluated. A K 2 TeO 3 concentration of 60 ppm was found to favor the growth of agrobacteria and restrict the development of other bacteria. Morphotypic analyses were used to define agrobacterial colony types, which were readily distinguished from other colonies. The typical agrobacterial morphotype allowed direct determination of the densities of agrobacterial populations from various environments on K 2 TeO 3 -amended medium. The bona fide agrobacterium colonies growing on media amended with K 2 TeO 3 were confirmed to be Agrobacterium colonies by using 16S ribosomal DNA (rDNA) probes. Specific 16S rDNA probes were designed for Agrobacterium biovar 1 and related species (Agrobacterium rubi and Agrobacterium fici) and for Agrobacterium biovar 2. Specific pathogenic probes from different Ti plasmid regions were used to determine the pathogenic status of agrobacterial colonies. Various morphotype colonies from bulk soil suspensions were characterized by colony blot hybridization with 16S rDNA and pathogenic probes. All the Agrobacterium-like colonies obtained from soil suspensions on amended media were found to be bona fide agrobacteria. Direct colony counting of agrobacterial populations could be done. We found 10 3 to 10 4 agrobacteria ⅐ g of dry soil ؊1 in a silt loam bulk soil cultivated with maize. All of the strains isolated were nonpathogenic bona fide Agrobacterium biovar 1 strains.

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confidence: 49%

Novel Tellurite-Amended Media and Specific Chromosomal and Ti Plasmid Probes for Direct Analysis of Soil Populations of Agrobacterium Biovars 1 and 2

Mougel

Cournoyer

Nesme

2001

Appl Environ Microbiol

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“…When selective media were used. the populations of Agrobacterium were reported to be high in orchards and nonagricultural areas (KERR 1969;SCHROTH et at 1971;SPIERS 1979;BOUZAR and MOORE 1987). However.…”

Section: Characteristics Of the Bacte;iamentioning

confidence: 99%

Characteristics of bacteria isolated from soil and roots of fruit trees

Yasuda

Katoh

1989

Soil Science and Plant Nutrition

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“…Recently, crown gall disease has been increasing in apple nurseries in Japan (Nekoduka et al, 2001). To succeed in growing intact saplings, soil fumigation is effective as a means of control, but it has been claimed that soil fumigation does not entirely prevent crown gall disease (Schroth et al, 1971). Use of resistant rootstocks is an alternative method for controlling crown gall disease.…”

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confidence: 99%

Evaluation and Inheritance of Crown Gall Resistance in Apple Rootstocks

Moriya

Iwanami

Takahashi

et al. 2008

J. Japan. Soc. Hort. Sci.

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To identify sources of resistance to crown gall disease and to investigate its inheritance pattern to descendants, we assessed the degree of resistance among seven apple rootstocks of 'JM5', 'JM7', 'M. 9', 'M. 27', 'G. 65', Malus prunifolia 'Mo 84a', and 'Morioka Seishi', two wild Malus accessions of Malus sieboldii 'Sanashi 63' and 'Mo-15', and its hybrids (147 individuals). The inoculation was tested using two Agrobacterium tumefaciens strains of Peach CG8331 (biovar 2) and ARAT-001 (biovar 1) as inocula. M. sieboldii 'Sanashi 63' and 'Mo-15' did not show any galls at the inoculated sites six months after inoculation with suspensions of the strain Peach CG8331. Galls developed on the other rootstocks with a frequency from 0.31 to 0.82. In an inoculation test with strain ARAT-001 as the inoculum, no galls were formed on M. sieboldii 'Mo-15', and the frequency of M. sieboldii 'Sanashi 63' was low, 0.19. The frequency in 'G. 65' inoculated with strain ARAT-001 was much lower than that with strain Peach CG8331, whereas that in other rootstocks showed similar or higher frequency compared to strain Peach CG8331. The results suggested that there is an interaction (specificity) for the frequency of gall occurrence between A. tumefaciens strain and apple rootstocks. Based on the results of our study, M. sieboldii 'Sanashi 63' and 'Mo-15' were regarded as the most resistant genotypes to the virulent strains of A. tumefaciens used in our study. Resistant hybrids with no galls were found in progeny derived from a cross between 'JM7' × 'Sanashi 63' against strains of A. tumefaciens; numbers of hybrids were 19 (16%) and 5 (4%) against strains Peach CG8331 and ARAT-001, respectively. In F 1 progeny between 'JM5' × 'G. 65', plants with no galls were not observed. These results indicate that crown gall resistance in M. sieboldii 'Sanashi 63' is heritable through its descendants.

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Biology and control ofAgrobacterium tumefaciens

Cited by 47 publications

References 11 publications

Novel Tellurite-Amended Media and Specific Chromosomal and Ti Plasmid Probes for Direct Analysis of Soil Populations of Agrobacterium Biovars 1 and 2

Novel Tellurite-Amended Media and Specific Chromosomal and Ti Plasmid Probes for Direct Analysis of Soil Populations of Agrobacterium Biovars 1 and 2

Characteristics of bacteria isolated from soil and roots of fruit trees

Evaluation and Inheritance of Crown Gall Resistance in Apple Rootstocks

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