Biology and principles of periodontal wound healing/regeneration

Polimeni, Giuseppe; Xiropaidis, Andreas V.; Wikesjö, Ulf M.E.

doi:10.1111/j.1600-0757.2006.00157.x

Cited by 408 publications

(408 citation statements)

References 54 publications

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“…No inflammatory response was determined disclosing the biocompatibility of the used materials. Remarkable was the better wettability of the PLGA/TCP composite in comparison to the PLGA only material, since it could increase the stabilization of the blood clot needed for optimal bone regeneration [41].…”

Section: Discussionmentioning

confidence: 99%

In vivo and in vitro evaluation of flexible, cottonwool-like nanocomposites as bone substitute material for complex defects

et al. 2009

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In vivo and in vitro evaluation of flexible, cottonwool-like nanocomposites as bone substitute material for complex defects AbstractThe easy clinical handling and applicability of biomaterials has become a focus of materials research due to rapidly increasing time and cost pressures in the public health sector. The present study assesses the in vitro and in vivo performance of a flexible, mouldable, cottonwool-like nanocomposite based on poly(lactide-co-glycolide) and amorphous tricalcium phosphate nanoparticles (PLGA/TCP 60:40). Immersion in simulated body fluid showed exceptional in vitro bioactivity for TCP-containing fibres (mass gain: 18%, 2 days, HAp deposition). Bone regeneration was quantitatively investigated by creating four circular non-critical-size calvarial defects in New Zealand White rabbits. The defects were filled with the easy applicable cottonwool-like PLGA/TCP fibres or PLGA alone. Porous bovine-derived mineral (Bio-Oss) was used as a positive control and cavities left empty served as a negative control. The area fraction of newly formed bone (4 weeks implantation) was significantly increased for TCP-containing fibres compared to pure PLGA (histological and micro-computed tomographic analysis). A spongiosa-like structure of the newly formed bone tissue was observed for PLGA/TCP nanocomposites, whereas Bio-Oss-treated defects afforded a solid cortical bone.-1-

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Section: Discussionmentioning

confidence: 99%

In vivo and in vitro evaluation of flexible, cottonwool-like nanocomposites as bone substitute material for complex defects

et al. 2009

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“…3 The limited regeneration potential of current techniques may be a result of poor innate ability of damaged periodontal tissues to regenerate. 4 This may occur after the prolonged inflammatory process caused by pathogenic bacteria. Periodontopathic bacteria possess a number of potential virulence factors and induce host inflammatory mediators, eventually leading to connective tissue degradation and alveolar bone resorption.…”

Section: Introductionmentioning

confidence: 99%

Osteogenic potential of periodontal ligament stem cells are unaffected after exposure to lipopolysaccharides

Albiero¹,

Amorim²,

Casati³

et al. 2017

Braz. oral res.

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Periodontitis develops as a result of a continuous interaction between host cells and subgingival pathogenic bacteria. The periodontium has a limited capacity for regeneration, probably due to changes in periodontal ligament stem cells (PDLSCs) phenotype. The aim of this study was to evaluate the effects of lipopolysaccharides from Porphyromonas gingivalis (PgLPS) on mesenchymal phenotype and osteoblast/cementoblast (O/C) potential of PDLSCs. PDLSCs were assessed for Toll-like receptor 2 (TLR2) expression by immunostaining technique. After, cells were exposed to PgLPS, and the following assays were carried out: (i) cell metabolic activity using MTS; (ii) gene expression for IL-1β, TNF-α and OCT-4 by real-time polymerase chain reaction (RT-qPCR); (iii) flow cytometry for STRO-1 and CD105, and (iv) osteogenic differentiation. PDLSCs were positive for TLR2. PgLPS promoted cell proliferation, produced IL-1β and TNF-α, and did not affect the expression of stem cell markers, STRO-1, CD105 and OCT-4. Under osteogenic condition, PDLSCs exposed to PgLPS showed a similar potential to differentiate toward osteoblast/cementoblast phenotype compared to control group as revealed by mineralized matrix deposition and levels of transcripts for RUNX2, ALP and OCN. These results provide evidence that PgLPS induces pro-inflammatory cytokines, but does not change the mesenchymal phenotype and osteoblast/cementoblast differentiation potential of PDLSCs.

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“…However, residual mid buccal recession could be treated with a second-step mucogingival surgical procedure, laterally positioned flap-free gingival graft combination. Two-wall infrabony defects do not provide the best three dimensional architecture for vascular and cellular bridging of the defect that originates from adjacent alveolar bone and periodontal ligament and especially in wide and non-containing two-wall defects the space maintenance cannot be provided satisfactorily by the defect walls for increasing the retention and minimizing the collapse of the materials [21,22]. Although the treated defect was partially supraalveolar and two-wall infrabony part was extending to the apex in combination with a buccal dehiscence, results of the exhibited twostep treatment revealed an obvious improvement of periodontal variables and this improvement might be associated with the positive effect of PRF to the cellular events during early healing period by its growth factor content.…”

Section: Discussionmentioning

confidence: 99%

“…Many years before, the key role of space provision and wound stability in regenerative treatment was reported by some studies evaluating the guided tissue regeneration techniques and as verified, possibility of bone formation increases by providing space provision without rigorous interruption of the gingival connective tissue [21,24,25]. PRF was covered over the defect as a membrane in the first surgical step of the present case, and the role that was imputed to the PRF membrane was containing the infrabony part of the defect in the early phase of healing due to this principle.…”

Section: Discussionmentioning

confidence: 99%

Management of Two-Wall Infra-Bony Defect with Platelet Rich Fibrin and Connective Tissue Graft Combination: A Case Report

Keçeli¹

2015

J Dent Probl Solut

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Background: Regenerative procedures offer a limited potential toward achieving a complete periodontal regeneration and today, there is not any single material that can be considered as 'gold standard' in infra bony defect treatment. Platelet-rich fibrin (PRF) is the second-generation platelet concentrate and represents a slow release system for growth factors. Use of PRF showed promising results in various periodontal surgical procedures. The aim of this report is to describe two-stage treatment of a canine tooth having an infrabony defect accompanied with a deep dehiscence by using PRF-connective tissue graft (CTG) combination.Case Report: A 28-year old man was referred to the Department of Periodontology with chief complaints of purulent exudate formation, tooth mobility in tooth #23 (FDA classification). Deep periodontal pockets in combination with 4 mm buccal recession were detected. Radiographically, a significant radiolucency was also present. CTG graft combined with coronally advanced flap by adding a PRF gel was utilized for the treatment of defect and accompanying gingival recession. Conclusion:Significant improvements were detected at all periodontal clinical and radiographic measurements.

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Biology and principles of periodontal wound healing/regeneration

Cited by 408 publications

References 54 publications

In vivo and in vitro evaluation of flexible, cottonwool-like nanocomposites as bone substitute material for complex defects

In vivo and in vitro evaluation of flexible, cottonwool-like nanocomposites as bone substitute material for complex defects

Osteogenic potential of periodontal ligament stem cells are unaffected after exposure to lipopolysaccharides

Management of Two-Wall Infra-Bony Defect with Platelet Rich Fibrin and Connective Tissue Graft Combination: A Case Report

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