Biology, fisheries, and conservation of sturgeons and paddlefish in China

Wei, Qiwei; Ke, Fu'en; Zhang, Jueming; Zhuang, Ping; Luo, Junde; Zhou, Rueqiong; Yang, Wenhua

doi:10.1007/0-306-46854-9_14

Cited by 150 publications

(265 citation statements)

References 6 publications

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“…Thus, we chose the Chinese sturgeon (Acipenser sinensis) to explore the origin and evolution of DAZ family genes in vertebrates. On the other hand, Chinese sturgeon is a rare and endangered species, due to anthropogenic interference, such as over-fishing, damming of rivers and pollution (Birstein et al 1997;Wei et al 1997). Controlled propagation has been successfully conducted (Wei et al 2013) and proven to be important for its conservation.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Differential expression of fertility genes boule and dazl in Chinese sturgeon (Acipenser sinensis), a basal fish

Yue

et al. 2015

Cell Tissue Res

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The gene family DAZ (deleted in Azoospermia), including boule, dazl and DAZ, performs highly conserved functions in germ cell development and fertility across animal phyla. Differential expression patterns have been demonstrated for the family members in invertebrates and vertebrates including fish. Here, we report the identification of boule and dazl and their expression at both RNA and protein levels in developing and mature gonads of Chinese sturgeon (Acipenser sinensis). Firstly, the isolation of the boule and dazl genes in Chinese sturgeon and the observation of the two genes in coelacanth suggest that dazl originated after the divergence of bony fish from cartilaginous fish but before the emergence of the Actinistia. Quantitative real-time PCR and western blot analyses reveal that boule and dazl RNA and proteins are restricted to the testis and ovary. In situ hybridization and fluorescent immunohistochemistry show that the bisexual mitotic and meiotic germ cell expression of dazl RNA and protein is conserved in vertebrates, while Chinese sturgeon boule RNA and protein exhibit mitotic and meiotic expression in the testis, and also likely display mitotic and meiotic expression in female. Moreover, we directly demonstrate for the first time that sturgeon Balbiani body/mitochondrial cloud disperses in the cytoplasm of early developing oocytes and co-localizes with Dazl to some extent. Finally, urbilaterian boule may also have an ancestral function in oogenesis. Taken together, these results provide useful information on the evolution of DAZ family genes, expression patterns and functions in animal reproduction.

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Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Differential expression of fertility genes boule and dazl in Chinese sturgeon (Acipenser sinensis), a basal fish

Yue

et al. 2015

Cell Tissue Res

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“…It belongs to the Acipenseriformes, a group of Chondrichthyans with an evolutionary history of over 140-160 million years. Since 1980s, its stock has declined dramatically due to overfishing, loss of natural habitat for reproduction and interference by other human activities [1,2]. Nowadays, the species was listed as a Grade I protected animal in China and Appendix II species in the CITES (the Convention on International Trade in Endangered Species of Wild Fauna and Flora).…”

Section: Introductionmentioning

confidence: 99%

EST dataset of pituitary and identification of somatolactin and novel genes in Chinese sturgeon, Acipenser sinensis

Cao

Leng

et al. 2011

Mol Biol Rep

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Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry, however, a few genes have been identified in this species. We report here construction of a pituitary cDNA library from a 24 years old female Chinese sturgeon just after its spawning, and obtained 2,025 ESTs from the library. 885 unique sequences were identified, which were categorized into 12 functional groups. More than half of the unique sequences (57%) do not match with annotated sequences in the public databases. Three of these novel genes were further identified. Notably, a full-length of cDNA (1,143 bp) encoding somatolactin of 232 amino acids was identified. Phylogenetic analysis showed 97% amino acid identity with White sturgeon somatolactin. RT-PCR analysis indicated that the somatolactin mRNA was only detected in pituitary. Pituitary-specific expression of the somatolactin suggested that the protein may play important physiological functions in pituitary-endocrine system of the Chinese sturgeon.

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“…Chinese sturgeon (Acipenser sinensis) is one of the Acipenseriformes, with an evolutionary history of over 200 million years (Wei et al 1997;Birstein et al 1997). Comparable to other sturgeon species, its stock has declined dramatically due to overfishing, loss of natural habitat for reproduction, and other anthropogenic interferences (Wei et al 1997;Billard and Lecointre 2001;Chen 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Comparable to other sturgeon species, its stock has declined dramatically due to overfishing, loss of natural habitat for reproduction, and other anthropogenic interferences (Wei et al 1997;Billard and Lecointre 2001;Chen 2007). To save this species more efficiently and to be able to develop an aquaculture industry, artificial propagation has been attempted since 1983.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and expression pattern of three zona pellucida 3 genes in the Chinese sturgeon, Acipenser sinensis

Wei

Chen

et al. 2010

Fish Physiol Biochem

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Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry. SMART cDNAs were synthesized from the ovary of A. sinensis, and the full-length cDNAs of three zona pellucida glycoprotein 3 genes (the new gene named AsZP3) were cloned and sequenced. AsZP3.1, AsZP3.2, and AsZP3.3 were 1,388 base pairs (bp), 1,288, and 1,290 bp in length, respectively, and they could be translated into proteins with 440, 394, and 398 amino acids, respectively. High level of amino acids sequence identity was seen between AsZP3.2 and AsZP3.3 (about 82%), but they share low identity with AsZP3.1 (26 and 23%, respectively). The AsZP3.1 has 42-50% amino acids sequence identity values with other fish and lower values with higher vertebrates (38%); AsZP3.2 and AsZP3.3 shared about 30-44% sequence identity with higher vertebrates and other fish. RT-PCR analysis indicated that AsZP3.1 displayed a wide tissue distribution at the mRNA levels including liver, kidney, spleen, heart, and ovary, but AsZP3.2 and AsZP3.3 mRNAs were expressed exclusively in the gonad. All three AsZP3 mRNAs were not detected during embryogenesis and early larval development; furthermore, they were not detected in the gonads of 1-and 2-year-old Chinese sturgeons. All three AsZP3 mRNAs were detected in the testes of 3-year-old males and in the ovaries of 4-and 5-year-old female Chinese sturgeons.

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Biology, fisheries, and conservation of sturgeons and paddlefish in China

Cited by 150 publications

References 6 publications

Differential expression of fertility genes boule and dazl in Chinese sturgeon (Acipenser sinensis), a basal fish

Differential expression of fertility genes boule and dazl in Chinese sturgeon (Acipenser sinensis), a basal fish

EST dataset of pituitary and identification of somatolactin and novel genes in Chinese sturgeon, Acipenser sinensis

Molecular characterization and expression pattern of three zona pellucida 3 genes in the Chinese sturgeon, Acipenser sinensis

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