Biology of
            <i>Borrelia hermsii</i>
            in Kelly Medium

Stoenner, Herbert G.

doi:10.1128/am.28.4.540-543.1974

Cited by 32 publications

(1 citation statement)

References 3 publications

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“…In 1971 Kelly obtained an unambiguous and repeatable culture of Borrelia in an artificial medium [40], including N-acetylglucosamine as the a constituent element of peptidoglycan, which is an "ingredient" of Borrelia cell wall. Three years later, Stönner used Kelly's medium to study the biology of Borrelia hermsii and noted that several hundred Borreliae were required in animal plasma to establish the spirochaetes growth in culture medium [41]. After several steps it was possible to recover "adapted" strains, suggesting that there are variants in the Borreliae population that are more suitable for living in test tubes.…”

Section: Spirochetosis From the Clinic To Culture Isolationmentioning

confidence: 99%

Borreliae Part 1: Borrelia Lyme Group and Echidna-Reptile Group

Trevisan

Cinco

Trevisini

et al. 2021

Biology

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Borreliae are divided into three groups, namely the Lyme group (LG), the Echidna-Reptile group (REPG) and the Relapsing Fever group (RFG). Currently, only Borrelia of the Lyme and RF groups (not all) cause infection in humans. Borreliae of the Echidna-Reptile group represent a new monophyletic group of spirochaetes, which infect amphibians and reptiles. In addition to a general description of the phylum Spirochaetales, including a brief historical digression on spirochaetosis, in the present review Borreliae of Lyme and Echidna-Reptile groups are described, discussing the ecology with vectors and hosts as well as microbiological features and molecular characterization. Furthermore, differences between LG and RFG are discussed with respect to the clinical manifestations. In humans, LG Borreliae are organotropic and cause erythema migrans in the early phase of the disease, while RFG Borreliae give high spirochaetemia with fever, without the development of erythema migrans. With respect of LG Borreliae, recently Borrelia mayonii, with intermediate characteristics between LG and RFG, has been identified. As part of the LG, it gives erythema migrans but also high spirochaetemia with fever. Hard ticks are vectors for both LG and REPG groups, but in LG they are mostly Ixodes sp. ticks, while in REPG vectors do not belong to that genus.

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Section: Spirochetosis From the Clinic To Culture Isolationmentioning

confidence: 99%

Borreliae Part 1: Borrelia Lyme Group and Echidna-Reptile Group

Trevisan

Cinco

Trevisini

et al. 2021

Biology

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Borrelia burgdorferi: another cause of foodborne illness?

Farrell¹,

Marth²

1991

International Journal of Food Microbiology

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Colony formation in solid medium by the relapsing fever spirochetes Borrelia hermsii and Borrelia turicatae

Raffel

Williamson

Schwan

et al. 2018

Ticks and Tick-borne Diseases

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Relapsing fever (RF) in North America is caused primarily by the spirochete Borrelia hermsii and is associated with the bite of its tick vector Ornithodoros hermsi. Although this spirochete was known long before the discovery of the Lyme disease (LD) spirochete, Borrelia burgdorferi, basic methods to facilitate the study of B. hermsii have lagged behind. One important technique to expedite the study of the molecular biology and pathogenesis of B. hermsii would be a reliable method to grow and clone these bacteria in solid medium, which we now describe. We have defined the solidifying agent, plating temperature, oxygen concentration, and pH for the efficient plating of two species of RF spirochetes, B. hermsii and Borrelia turicatae. Importantly, this technique allowed us to successfully isolate virulent, clonal cell lines of spirochetes, and to enumerate and isolate viable B. hermsii from infected mouse blood and tick tissues. Our results also demonstrate the value of testing a range of several environmental variables to increase the efficiency of bacterial isolation, which may be helpful for researchers working on other prokaryotes that are intractable for in vitro growth.

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Biology of Borrelia hermsii in Kelly Medium

Cited by 32 publications

References 3 publications

Borreliae Part 1: Borrelia Lyme Group and Echidna-Reptile Group

Borreliae Part 1: Borrelia Lyme Group and Echidna-Reptile Group

Borrelia burgdorferi: another cause of foodborne illness?

Colony formation in solid medium by the relapsing fever spirochetes Borrelia hermsii and Borrelia turicatae

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