Bioluminescence in analytical chemistry and in vivo imaging

Roda, Aldo; Guardigli, Massimo; Michelini, Elisa; Mirasoli, Mara

doi:10.1016/j.trac.2008.11.015

Cited by 157 publications

(96 citation statements)

References 85 publications

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“…Later, it was modified by different researchers and adapted for their specific purposes [2][3][4][5][6]. Now, the bacterial bioluminescent assay is a traditional and important biotechnological application of the bioluminescence phenomenon [7][8][9][10][11]. The tested physiological parameter here is luminescence intensity that can be easily measured instrumentally.…”

Section: Introductionmentioning

confidence: 99%

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Rozhko

Devyatlovskaya

Kudryasheva³

2014

Open Life Sciences

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Effects of Americium-241 (241Am), alpha-emitting radionuclide of high specific radioactivity, and tritium (3H), beta-emitting radionuclide, on luminous bacteria Photobacterium phosphoreum were compared. Bioluminescence intensity served as a marker of bacterial physiological activity. Three successive stages in the bioluminescence response to 241Am and 3H were found under conditions of lowdose irradiation: (1) absence of effects, (2) activation, and (3) inhibition. They were interpreted in terms of bacterial response to stressfactor as stress recognition, adaptive response/syndrome, and suppression of physiological function (i.e. radiation toxicity). Times of bioluminescence activation (TBA) and inhibition (TBI) were suggested as parameters to characterize hormesis and toxic stages in a course of chronic low-dose irradiation of the microorganisms. Values of TBA and TBI of 241Am were shorter than those of 3H, revealing higher impact of alpha-irradiation (as compared to beta-irradiation) under comparable radiation doses. Increases of peroxide concentration and NADH oxidation rates in 241Am aquatic solutions were demonstrated; these were not found in tritiated water. The results reveal a biological role of reactive oxygen species generated in water solutions as secondary products of the radioactive decay. The study provides a scientific basis for elaboration of bioluminescence-based assay to monitor radiotoxicity of alpha- and beta-emitting radionuclides in aquatic solutions.

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Section: Introductionmentioning

confidence: 99%

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Rozhko

Devyatlovskaya

Kudryasheva³

2014

Open Life Sciences

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“…Ataluren | multisubstrate adduct inhibitor | protein stability | reporter gene assay | X-ray crystallography F irefly luciferase from Photinus pyralis (FLuc) is an ATP-dependent luciferase widely used as a reporter enzyme for cell-based gene expression assays, principally due to the high sensitivity and large dynamic range bioluminescence affords (1,2). However, as an enzyme that catalyzes the bimolecular reaction between two small molecule substrates, D-luciferin and ATP, it is prone to inhibition by a variety of low-molecular-weight heterocyclic compounds typically found in screening collections (3,4).…”

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confidence: 99%

Molecular basis for the high-affinity binding and stabilization of firefly luciferase by PTC124

Auld

Lovell

Thorne

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

164

161

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Firefly luciferase (FLuc), an ATP-dependent bioluminescent reporter enzyme, is broadly used in chemical biology and drug discovery assays. PTC124 (Ataluren; (3-[5-(2-fluorophenyl)-1,2,4-oxadiazol-3-yl] benzoic acid) discovered in an FLuc-based assay targeting nonsense codon suppression, is an unusually potent FLuc-inhibitor. Paradoxically, PTC124 and related analogs increase cellular FLuc activity levels by posttranslational stabilization. In this study, we show that FLuc inhibition and stabilization is the result of an inhibitory product formed during the FLuc-catalyzed reaction between its natural substrate, ATP, and PTC124. A 2.0 Å cocrystal structure revealed the inhibitor to be the acyl-AMP mixed-anhydride adduct PTC124-AMP, which was subsequently synthesized and shown to be a highaffinity multisubstrate adduct inhibitor (MAI; K D ¼ 120 pM) of FLuc. Biochemical assays, liquid chromatography/mass spectrometry, and near-attack conformer modeling demonstrate that formation of this novel MAI is absolutely dependent upon the precise positioning and reactivity of a key meta-carboxylate of PTC124 within the FLuc active site. We also demonstrate that the inhibitory activity of PTC124-AMP is relieved by free coenzyme A, a component present at high concentrations in luciferase detection reagents used for cell-based assays. This explains why PTC124 can appear to increase, instead of inhibit, FLuc activity in cell-based reporter gene assays. To our knowledge, this is an unusual example in which the "off-target" effect of a small molecule is mediated by an MAI mechanism.Ataluren | multisubstrate adduct inhibitor | protein stability | reporter gene assay | X-ray crystallography

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“…The additional push for one technology to monitor several biomarkers simultaneously for more effective and universal disease diagnosis is motivating recent advancements in multiplexed sensing capabilities. Many optical approaches have been utilized for POC applications including fluorescence [69][70][71][72], luminescence, absorbance, Forster energy transfer (FRET) [73], bioluminescence energy transfer (BRET) [74][75][76], surfaceplasmon resonance (SPR) [77,78], resonant Rayleigh scattering [79][80][81][82], geometric scattering [1], and Raman spectroscopic approaches [83][84][85][86][87][88][89]. Advancements in biochemical sensing methods, nanotechnology, and the miniaturization of optics have been key in improving spectroscopic platforms for biomarker detection.…”

Section: Optical Analyses For Facilitating Poc Technologiesmentioning

confidence: 99%

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

et al. 2017

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Point-of-care (POC) device development is a growing field that aims to develop low-cost, rapid, sensitive in-vitro diagnostic testing platforms that are portable, selfcontained, and can be used anywhere -from modern clinics to remote and low resource areas. In this review, surface enhanced Raman spectroscopy (SERS) is discussed as a solution to facilitating the translation of bioanalytical sensing to the POC. The potential for SERS to meet the widely accepted "ASSURED" (Affordable, Sensitive, Specific, Userfriendly, Rapid, Equipment-free, and Deliverable) criterion provided by the World Health Organization is discussed based on recent advances in SERS in vitro assay development. As SERS provides attractive characteristics for multiplexed sensing at low concentration limits with a high degree of specificity, it holds great promise for enhancing current efforts in rapid diagnostic testing. In outlining the progression of SERS techniques over the past years combined with recent developments in smart nanomaterials, high-throughput microfluidics, and low-cost paper diagnostics, an extensive number of new possibilities show potential for translating SERS biosensors to the POC.

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Bioluminescence in analytical chemistry and in vivo imaging

Cited by 157 publications

References 85 publications

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Molecular basis for the high-affinity binding and stabilization of firefly luciferase by PTC124

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

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