Bioluminescent Probe for Hydrogen Peroxide Imaging in Vitro and in Vivo

Abstract

Reactive oxygen species (ROS) often have significant roles in mediating redox modifications and other essential physiological processes, such as biological process regulation and signal transduction. Considering that H2O2 is a substantial member of ROS, detection and quantitation of H2O2 undertakes important but urgent responsibility. In this report, a bioluminescent probe for detecting H2O2 was well designed, synthesized, and evaluated. This probe was designed into three parts: a H2O2-sensitive aryl boronic acid, a bioluminescent aminoluciferin moiety, and a self-immolative linker. After extensive evaluation, this probe can selectively and sensitively react with H2O2 to release aminoluciferin. It should be pointed out that this probe is a potential bioluminescent sensor for H2O2 since it can provide a promising toolkit for real-time detection of the H2O2 level in vitro, in cellulo, and in vivo.

Discovery of a series of 2-phenylnaphthalenes as firefly luciferase inhibitors

“…[40][41][42] To avoid the individual variation of mouse, we tested the bioluminescence signal of total ux (photons per s per cm 2 per steradian) by injecting aminoluciferin intraperitoneally into the mouse on the rst day, and set it as the calibration value. [40][41][42] To avoid the individual variation of mouse, we tested the bioluminescence signal of total ux (photons per s per cm 2 per steradian) by injecting aminoluciferin intraperitoneally into the mouse on the rst day, and set it as the calibration value.…”

Preparation of a fluorescent silver nanoprism–dye complex for detection of hydrogen peroxide in milk

“…2 Numerous methods have been developed to detect H 2 O 2 such as spectrophotometry, luminescence, and electrochemistry. 9 Several methods have also been proposed for the determination of H 2 O 2 in milk. 10 , such as oxygen electrode method, flow injection analysis, FT-IR method and amperometric biosensor.…”

P1‐104: Imaging Hydrogen Peroxide in Alzheimer’s Disease Via Cascade Signal Amplification

“…As illustrated in Fig. 1a [21][22][23][24][25][26][27] . Clearly, it is an urgent need to provide an in vivo imaging tool to detect the H 2 O 2 concentration in the living brain, particularly AD brains.…”