Biomanufacturing of 3D Tissue Constructs in Microgravity and their Applications in Human Pathophysiological Studies

Ren, Zhanping; Harriot, Anicca D.; Mair, Devin B.; Chung, Michael K.; Lee, Peter H. U.; Kim, Deok‐Ho

doi:10.1002/adhm.202300157

Cited by 10 publications

(2 citation statements)

References 181 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, 3D in vitro models have been proposed to study the molecular mechanisms of tissue regulation in low gravity [ 3 ], with appropriate limitations. Regarding in vivo animal models, rodents have been often used in ground experiments.…”

Section: Introductionmentioning

confidence: 99%

Vibration Rather than Microgravity Affects Bone Metabolism in Adult Zebrafish Scale Model

Carnovali,

Zava,

Banfi

et al. 2024

Cells

View full text Add to dashboard Cite

Gravity and mechanical forces cause important alterations in the human skeletal system, as demonstrated by space flights. Innovative animal models like zebrafish embryos and medaka have been introduced to study bone response in ground-based microgravity simulators. We used, for the first time, adult zebrafish in simulated microgravity, with a random positioning machine (RPM) to study bone remodeling in the scales. To evaluate the effects of microgravity on bone remodeling in adult bone tissue, we exposed adult zebrafish to microgravity for 14 days using RPM and we evaluated bone remodeling on explanted scales. Our data highlight bone resorption in scales in simulated microgravity fish but also in the fish exposed, in normal gravity, to the vibrations produced by the RPM. The osteoclast activation in both rotating and non-rotating samples suggest that prolonged vibrations exposure leads to bone resorption in the scales tissue. Stress levels in these fish were normal, as demonstrated by blood cortisol quantification. In conclusion, vibrational mechanical stress induced bone resorption in adult fish scales. Moreover, adult fish as an animal model for microgravity studies remains controversial since fish usually live in weightless conditions because of the buoyant force from water and do not constantly need to support their bodies against gravity.

show abstract

Section: Introductionmentioning

confidence: 99%

Vibration Rather than Microgravity Affects Bone Metabolism in Adult Zebrafish Scale Model

Carnovali,

Zava,

Banfi

et al. 2024

Cells

View full text Add to dashboard Cite

show abstract

“…may offer for cardiovascular applications, such as for induced pluripotent stem cell-derived cardiomyocytes and organoids Ren et al (2023). have likewise recently highlighted in a…”

mentioning

confidence: 99%

The Effects of Simulated and Real Microgravity on Vascular Smooth Muscle Cells

Ludtka,

Allen

2024

Gravitational and Space Research

View full text Add to dashboard Cite

As considerations are being made for the limitations and safety of long-term human spaceflight, the vasculature is important given its connection to and impact on numerous organ systems. As a major constituent of blood vessels, vascular smooth muscle cells are of interest due to their influence over vascular tone and function. Additionally, vascular smooth muscle cells are responsive to pressure and flow changes. Therefore, alterations in these parameters under conditions of microgravity can be functionally disruptive. As such, here we review and discuss the existing literature that assesses the effects of microgravity, both actual and simulated, on smooth muscle cells. This includes the various methods for achieving or simulating microgravity, the animal models or cells used, and the various durations of microgravity assessed. We also discuss the various reported findings in the field, which include changes to cell proliferation, gene expression and phenotypic shifts, and renin-angiotensin-aldosterone system (RAAS), nitric oxide synthase (NOS), and Ca2+ signaling. Additionally, we briefly summarize the literature on smooth muscle tissue engineering in microgravity as well as considerations of radiation as another key component of spaceflight to contextualize spaceflight experiments, which by their nature include radiation exposure. Finally, we provide general recommendations based on the existing literature's focus and limitations.

show abstract

Satellite‐Based On‐Orbit Printing of 3D Tumor Models

Mo,

Zhang,

Wang

et al. 2023

Advanced Materials

View full text Add to dashboard Cite

Space three dimension (3D） bioprinting provides a precise and bionic tumor model for evaluating the compound effect of the space environment on tumors, thereby providing insight into the progress of the disease and potential treatments. However, space 3D bioprinting faces several challenges, including prelaunch uncertainty, possible liquid leakage, long‐term culture in space, automatic equipment control, data acquisition, and transmission. Here, a novel satellite‐based 3D bioprinting device with high structural strength, small volume, and low weight (<6 kg) is developed. A microgel‐based biphasic thermosensitive bioink and suspension medium that supports the on‐orbit printing and in situ culture of complex tumor models is developed. An intelligent control algorithm that enables the automatic control of 3D printing, autofocusing, fluorescence imaging, and data transfer back to the ground is developed. To the authors' knowledge, this is the first time that on‐orbit printing of tumor models is achieved in space with stable morphology and moderate viability via a satellite. It is found that 3D tumor models are more sensitive to antitumor drugs in space than on Earth. This study opens up a new avenue for 3D bioprinting in space and offers new possibilities for future research in space life science and medicine.

show abstract

Biomanufacturing of 3D Tissue Constructs in Microgravity and their Applications in Human Pathophysiological Studies

Cited by 10 publications

References 181 publications

Vibration Rather than Microgravity Affects Bone Metabolism in Adult Zebrafish Scale Model

Vibration Rather than Microgravity Affects Bone Metabolism in Adult Zebrafish Scale Model

The Effects of Simulated and Real Microgravity on Vascular Smooth Muscle Cells

Satellite‐Based On‐Orbit Printing of 3D Tumor Models

Contact Info

Product

Resources

About