Biomarker-based evaluation of cytogenotoxic potential of glyphosate in Vigna mungo (L.) Hepper genotypes

Khan, Adiba; Kumar, Vimal; Srivastava, Alaka; Saxena, Gauri; Verma, Praveen Chandra

doi:10.1007/s10661-021-08865-x

Cited by 11 publications

(6 citation statements)

References 72 publications

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“…Alvarez-Moya et al [34] reported the genotoxicity of G in the blood of humans in a mean concentration of 0.0007-0.7 mM. To ensure the analysis of genotoxic activity, high concentrations (50 and 10 mM) and lower ones such as those reported by Khan et al [7] were used, observing the dose-dependent genotoxic activity of G, from 0.1 mM-50 mM, using the tail length, tail moment, and MG parameters. Some reports suggest that the genotoxic activity of GBH is due to the adjuvants used and not to G [1,20].…”

Section: Discussionmentioning

confidence: 98%

“…Although there is evidence of the genotoxic activity of G [7,8], this is controversial [16,18], and it is necessary to increase the degree of certainty of its genotoxicity. Alvarez-Moya et al [34] reported the genotoxicity of G in the blood of humans in a mean concentration of 0.0007-0.7 mM.…”

Section: Discussionmentioning

confidence: 99%

“…Human exposure to CFG occurs during occupational and environmental activities and a maximum urinary glyphosate concentration of 1.7 times above the recommended acceptable daily intake has been reported [6]. There is abundant evidence of G genotoxicity [7][8][9][10] and its effect on the expression of some genes and in human health [11,12]. Additionally, G represents a risk for other living organisms [13].…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Genetic Damage Induced via Glyphosate and Three Commercial Formulations with Adjuvants in Human Blood Cells

Alvarez-Moya

Reynoso-Silva

2023

IJMS

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There is considerable controversy regarding the genotoxicity of glyphosate (N-(phosphonomethyl) glycine). It has been suggested that the genotoxicity of this herbicide is increased by the adjuvants added to commercial formulations based on glyphosate. The effect of various concentrations of glyphosate and three commercial glyphosate-based herbicides (GBH) on human lymphocytes was evaluated. Human blood cells were exposed to glyphosates of 0.1, 1, 10 and 50 mM as well as to equivalent concentrations of glyphosate on commercial formulations. Genetic damage (p < 0.05) was observed in all concentrations with glyphosate and with FAENA and TACKLE formulations. These two commercial formulations showed genotoxicity that was concentration-dependent but in a higher proportion compared to pure glyphosate only. Higher glyphosate concentrations increased the frequency and range of tail lengths of some migration groups, and the same was observed for FAENA and TACKLE, while in CENTELLA the migration range decreased but the frequency of migration groups increased. We show that pure glyphosate and commercial GBH (FAENA, TACKLE and CENTELLA) gave signals of genotoxicity in human blood samples in the comet assay. The genotoxicity increased in the formulations, indicating genotoxic activity also in the added adjuvants present in these products. The use of the MG parameter allowed us to detect a certain type of genetic damage associated with different formulations.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of Genetic Damage Induced via Glyphosate and Three Commercial Formulations with Adjuvants in Human Blood Cells

Alvarez-Moya

Reynoso-Silva

2023

IJMS

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“…Cytological observations were recorded using temporary slide preparations to screen mitotic activity/(MI), micronuclei, and several CAs. Approximately 1000 cells from at least 10 well smeared microscopic elds out of 20 elds captured from different root meristems preparation per treatment were screened at 400 x under the light microscope (Magnus MLX-TR Plus) (Khan et al 2021). MI percentage, CAs and cells with micronuclei were calculated in reference to total number of cells in a microscopic eld.…”

Section: Fixing Of Roots and Analysis Of Genotoxicitymentioning

confidence: 99%

Copper Oxide Nanoparticles and Bulk Particles Stress Induced Cytological and Physiological Responses of Vicia Faba L.

Kumar

Srivastava

et al. 2021

Preprint

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CuO nanoparticles (NPs) and their bulk counter parts are being utilized in various industrial preparations. The progressive increase in the use of CuO NPs and bulk particles (BPs) eventually ends up in the environment, causing potential hazard to biota and imbalance in the abiotic components. In order to elucidate the toxic impact of CuO NPs and BPs, plant seedlings of Vicia faba var. Pusa Sumit were exposed to 20-100 mg L⁻1 of CuO NPs and BPs along with a control set up. Root tips and leaf tissues of plant seedlings were used to perform genotoxic and biochemical assays, respectively. Cytological preparations were used to screen mitotic indices (MI), micronuclei and chromosomal abnormalities (CAs). CuO NPs treatment led to 24.1 % reduction in MI and 7.9 % increase in CAs while BPs treatment reduced MI by 12.7 % and raised CAs by 4.3 % only. Bio-uptake of CuO NPs and BPs in the plant tissues is the key cause of oxidative stress. It triggered significant changes in lipid peroxidation and other biochemical parameters including enzymatic (peroxidase, superoxide dismutase, catalase, glutathione s-transferase and glutathione reductase) and non-enzymatic (photosynthetic pigments and proline content) components of antioxidant system in treated plant seedlings. In this study, CuO NPs caused 49.1 % to 96.7 % enhanced activity of antioxidant enzymes as compared to BPs. These findings revealed that CuO NPs were more toxic to plants than their counter BPs.

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“…Many plant genotoxicity tests have been developed to investigate the cytogenetic effects of various agents that have the potential to damage DNA (physical, chemical). Chemical mutagens with different mechanisms of action include maleic hydrazide (MH), a clastogenic agent that can cause chromosome breaks as well as defects in spindle fibers [9], and nitroso-N-methylurea (MNU), an alkylating agent that primarily causes gene mutations but also causes chromosomal aberra-tions. The mutagens used in this study act at different stages of the cell cycle, with MH acting during the S phase and MNU acting during the G2 phase [10,11].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Mutagens on Callus Regeneration

Iksat¹,

Masalimov²,

Omarov³

2022

Eurasian Journal of Applied Biotechnology

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in this work were studied the characteristics of the development of F. tataricum and F. esculentum callus cultures in vitro when they were treated with chemical mutagen solutions - MH, MNU (0.05; 0.1; 0.2; 0.5; 1; 2 mM). When compared to the control on RX medium, the calli treated with the MNU mutagen died more often at higher concentrations (0.5; 1; 2 mM), indicating the effectiveness of the mutagenic effect. The survival rate of callus cultures subcultivated on a medium containing lower concentrations of mutagens (0.05; 0.1; 0.2 mM) was quite high when compared to higher concentrations, but low when compared to the control. The role of mutagenic factors in increasing genotypic variability, as well as the method of obtaining a new source material for various aspects of the buckwheat breeding process, have not been studied.

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Biomarker-based evaluation of cytogenotoxic potential of glyphosate in Vigna mungo (L.) Hepper genotypes

Cited by 11 publications

References 72 publications

Assessment of Genetic Damage Induced via Glyphosate and Three Commercial Formulations with Adjuvants in Human Blood Cells

Assessment of Genetic Damage Induced via Glyphosate and Three Commercial Formulations with Adjuvants in Human Blood Cells

Copper Oxide Nanoparticles and Bulk Particles Stress Induced Cytological and Physiological Responses of Vicia Faba L.

The Effect of Mutagens on Callus Regeneration

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