Biomarker responses of Cu-induced toxicity in European seabass Dicentrarchus labrax: Assessing oxidative stress and histopathological alterations

Díaz-de-Alba, Margarita; Canalejo, Antonio; Granado-Castro, M.D.; Ramírez, Milagrosa Oliva; El, Balabanova; García, Francisco Córdoba; Troyano-Montoro, M.; Espada-Bellido, Estrella; Santiago, Rafael Torronteras; Riaño, M. D. Galindo

doi:10.1016/j.marpolbul.2017.07.039

Cited by 17 publications

(5 citation statements)

References 64 publications

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“…The SOD enzyme acts as the first‐line defense mechanism against superoxide radicals. [ 65 ] In this study, decreased levels of SOD clearly show that normal oxidative phosphorylation is disrupted by swertiamarin. The CAT activity also decreased significantly compared with the control.…”

Section: Discussionmentioning

confidence: 58%

Developmental toxicity, antioxidant, and marker enzyme assessment of swertiamarin in zebrafish (Danio rerio)

Sakthivel

Samy

Dharaneedharan

2021

J Biochem & Molecular Tox

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A secoiridoid glycoside called swertiamarin has been widely used as a herbal medicine for many decades. In particular, swertiamarin from the Enicostema axillare herb has been used as a multipurpose drug to treat innumerable health problems. As this medicine is consumed orally, its toxicity level should be determined. To examine the safety of this compound, toxicology work was done in zebrafish, and this is the first report to describe swertiamarin toxicity in zebrafish. Zebrafish embryos were used in this swertiamarin toxicity study, and morphological changes were observed. Further, the compound was also studied in adult zebrafish to determine the impact of the compound on the fish liver.Enzyme profiling with superoxide dismutase, glutathione peroxidase, catalase, reduced glutathione levels, glutathione S-transferase, lactate dehydrogenase, glutamic oxaloacetic transaminases, lipid peroxidation, Na + /K + -ATPase, and glutamic pyruvic transaminases) was evaluated (p ≤ 0.05). Results suggest that swertiamarin is a safe drug only at a low concentration (40 µM). This study also shows that even herbal medicinal compounds may be toxic to humans at higher dosages. Hence, irrespective of whether a drug is synthetic or natural, it needs to be tested for its toxicity before use in humans.

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Section: Discussionmentioning

confidence: 58%

Developmental toxicity, antioxidant, and marker enzyme assessment of swertiamarin in zebrafish (Danio rerio)

Sakthivel

Samy

Dharaneedharan

2021

J Biochem & Molecular Tox

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“…This shows, on the one hand the susceptibility of common carp to elevated Cu and Cd concentrations and on the other hand the ability to quickly adapt to a stressful situation. In other studies, increases as well as decreases in CAT activity have been reported (Jia et al 2011, Díaz-de-Alba et al 2017, Pan et al 2018). However, our trend is in accordance with Pillet et al (2019) and indicates that common carp are able to adapt rapidly to metal ion exposure.…”

Section: Defensive Mechanismsmentioning

confidence: 64%

“…The aquatic environment is the main sink of pollutants produced by industries, sewage, agriculture and mining activities. Trace metals are persistent, non-degradable pollutants that can accumulate in the aquatic food web (Díaz-de-Alba et al 2017). Two groups of metals can be distinguished, namely essential and non-essential.…”

Section: Introductionmentioning

confidence: 99%

Antagonistic bioaccumulation of waterborne Cu(II) and Cd(II) in common carp (Cyprinus carpio) and effects on ion-homeostasis and defensive mechanisms

et al. 2020

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“…An inhibition of SOD activity by Cu was reported in three spine stickleback ( Gasterosteus aculeatus ) (Sanchez et al, 2005). In previous studies either an increase or a decrease of CAT activity was observed according to metal concentrations and tissues studied (Díaz‐de‐Alba et al, 2017; Jia, Zhang, & Liu, 2011; Pan et al, 2018). Regarding GST, an enzyme which conjugates GSH with electrophilic and other xenobiotics, a decline was recorded in different organisms exposed to various metal ions, such as Nile tilapia ( Oreochromis niloticus ) (Atli & Canli, 2010), the freshwater snail Lymnea luteola L. (Ali & Ali, 2015) and in common carp exposed to different concentrations of Cu (Dautremepuits, Paris‐Palacios, Betoulle, & Vernet, 2004; Pillet et al, 2019).…”

Section: Discussionmentioning

confidence: 89%

“…A similar pattern was observed in the liver. Inhibitory responses of antioxidant enzymes can be caused both by Cu—which binds the ‐SH group of the enzyme—or by the excess of ROS (Atli & Canli, 2007, 2010; Sanchez et al, 2005) and are indicative of contamination (Díaz‐de‐Alba et al, 2017; Hansen et al, 2007). An inhibition of SOD activity by Cu was reported in three spine stickleback ( Gasterosteus aculeatus ) (Sanchez et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

A comparative study on the effects of three different metals (Cu, Zn and Cd) at similar toxicity levels in common carp, Cyprinus carpio

Castaldo

Delahaut

Slootmaekers

et al. 2020

J of Applied Toxicology

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To improve our understanding of underlying toxic mechanisms, it is important to evaluate differences in effects that a variety of metals exert at concentrations representing the same toxic level to the organism. Therefore, the main goal of the present study was to compare the effects of waterborne copper (Cu(II)), zinc (Zn(II)) and cadmium (Cd (II)) on a freshwater fish, the common carp (Cyprinus carpio), at concentrations being 0%, 25%, 50% and 100% of the 96 h LC50 (the concentration which is lethal to 50% of the population in 96 h). All the exposures were performed for a period of 1 week at 20°C. Our results show a rapid increase in the amount of copper and cadmium accumulated in the gills, while zinc only started to increase by the end of the experiment. All three metal ions increased metallothionein gene expression in both gills and liver. However, clear adverse effects were mainly observed for the Cu exposed group. Cu caused a decrease in Na level in gill tissue; it altered the expression of genes involved in ionoregulation such as Na+/K+‐ATPase and H+‐ATPase as well as the expression of oxidative stress‐related genes, such as catalase, glutathione reductase and glutathione S‐transferase. Zinc and cadmium exposure did not alter the ion levels in the gills. In addition, no obvious effect of oxidative stress was observed, except for a transient increase in glutathione reductase at the highest cadmium concentration.

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Biomarker responses of Cu-induced toxicity in European seabass Dicentrarchus labrax: Assessing oxidative stress and histopathological alterations

Cited by 17 publications

References 64 publications

Developmental toxicity, antioxidant, and marker enzyme assessment of swertiamarin in zebrafish (Danio rerio)

Developmental toxicity, antioxidant, and marker enzyme assessment of swertiamarin in zebrafish (Danio rerio)

Antagonistic bioaccumulation of waterborne Cu(II) and Cd(II) in common carp (Cyprinus carpio) and effects on ion-homeostasis and defensive mechanisms

A comparative study on the effects of three different metals (Cu, Zn and Cd) at similar toxicity levels in common carp, Cyprinus carpio

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