A suite of low-mature
crude oils (five high-sulfur oils and six
low-sulfur oils) from the Huanghekou and the Laizhouwan Sags, Bohai
Bay Basin, are analyzed to investigate the fate of the hopanoids.
Abundant hopanes, such as secohopanes, 25-norhopanes, benzohopanes,
aromatized secohopanes, and sulfide hopanes, are identified, and their
carbon isotope compositions are determined. Varying 13C
isotope values of C31 hopane (−38.7–34.0‰)
and C29‑30 hopanes (−38.5–31.5‰)
suggest different bacterial sources of these compounds. The presence
of 25-norhopanes with enriched heavy carbon isotopes in severely biodegraded
oils suggests that they are microbially mediated products. The detection
of the isotopically depleted C29 and C30 D-ring-8,14-secohopanes
(−45.6–41.2‰) indicates that secohopanes are
from methane-oxidizing bacteria (methanotrophs). The presence of isorenieratane,
lower aryl isoprenoid ratios, and a good correlation between the sulfur
content and the gammacerane index indicate the presence of green sulfur
bacteria (Chlorobiaceae) under photic zone euxinic conditions. Water
column stratification results in good preservation of the organic
matter, and it is in favor of diversity of aquatic microorganisms.
The ratios of C35/C34 sulfide hopane, C35 sulfide hopane-2/C35 sulfide hopane-1, and C35/C34 benzohopane are influenced by the reducing
environments in this region. In addition, the D-ring monoaromatized
8,14-secohopanoid/(D-ring monoaromatized 8,14-secohopanoid + benzohopanes)
and C31–C35 secomoretanes/secohopanes
are affected by the maturity. We hypothesize that the reducing environments
and thermal effects are important markers for the hopanoid transformation,
including the incorporation of inorganic sulfur in substituting functional
groups, cyclizing, aromatizing, and opening ring C of the hopanoids.