Biomarkers at different levels of organisation after atrazine formulation (SIPTRAN 500SC®) exposure in Rhinella schineideri (Anura: Bufonidae) Neotropical tadpoles

Iglesias, Juan Manuel Pérez; Franco‐Belussi, Lilian; Natale, Guillermo Sebastián; Oliveira, Classius de

doi:10.1016/j.envpol.2018.10.073

Cited by 47 publications

(21 citation statements)

References 49 publications

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“…The use of morphological endpoints such as snout‐to‐vent length, body width and tail length has become more common in acute (96‐hour) ecotoxicological tests, using native amphibians (Cardoso‐Vera et al, ; Pérez‐Iglesias et al, ). Researchers must take care in discussing results from such multiple endpoints, assessed following both acute and chronic exposures, to safeguard against overinterpretation (i.e., making inferences from false‐positive results).…”

Section: Discussionmentioning

confidence: 99%

“…To meet our first objective, our design consisted of two experiments with 96‐hour acute ecotoxicity exposures (modified for native amphibian species) using three solvents: DMSO (Sigma‐Aldrich; purity ≥99.9%), ETOH (Commercial Alcohols; anhydrous ethyl alcohol, purity 100%) and ACE (Fisher Scientific; purity ≥99.5%). We selected GS 25 (Gosner, ) tadpoles following the recommendations using native amphibian species (Natale et al, ; Pérez‐Iglesias et al, ; Ruiz de Arcaute et al, ). In May 2017, three to five GS 25 tadpoles were haphazardly selected from two stock tanks and added to experimental 1‐L glass mason jars (Figure S1; see Supporting Information), containing 900 mL of dechloraminated tap water and varying concentrations of individual solvents.…”

Section: Methodsmentioning

confidence: 99%

“…For the acute exposures, we modified FETAX and standardized guidelines for aquatic toxicity testing (ATSM, 2007;ATSM, 2007), based on use of native species (Cardoso-Vera et al, 2017;Johansson, Piha, Kylin, & Merilä, 2006;Natale et al, 2018). Although conventional acute exposures focus on the lethality of test chemicals (LC 50 ), assessing additional endpoints (e.g., body length), has been widely used in many acute 96-192-hour amphibian toxicity tests for assessing sublethal effects of test chemicals such as growth inhibition of test subjects (Cardoso-Vera et al, 2017;Gutierrez-Villagomez et al, 2019;Pérez-Iglesias, Franco-Belussa, Natale, & de Oliveira, 2019;Ruiz de Arcaute, Salgado Costa, Demetrio, Natale, & Ronco, 2012). Early changes in amphibian larval development denoted by presence of abnormalities (e.g., stunted tails) induced by acute exposure to chemicals could have long-term effects under certain scenarios (Crespi & Denver, 2005).…”

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confidence: 99%

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Assessment of sublethal ecotoxicity of solvents on larvae of a model native amphibian (Lithobates pipiens)

Young

Gavel

Gutierrez-Villagómez

et al. 2019

J of Applied Toxicology

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Carrier solvents are used frequently in toxicity testing to assist hydrophobic chemicals into solution, but such solvents may have toxic effects on test subjects. Amphibians are model organisms in toxicity studies; however, little is known about the direct effects of solvents on native amphibians. Following modifications to standardized guidelines for native species, we used acute 96‐hour exposures to assess the direct effects of three common solvents on survival, differences in morphology and occurrence of abnormalities of northern leopard frog larvae (Lithobates pipiens). The solvents, dimethyl sulfoxide (DMSO), ethanol (ETOH) and acetone (ACE) were used at nominal concentrations ranging from 1 to 100 μL/L. We also conducted a 30‐day exposure to assess the direct chronic effects of DMSO at 1 and 5 μL/L, on larval growth, development and sex differentiation, but found no effects. Acute exposure to solvents also had no effect on the survival of larvae, but we found significant abnormalities in tadpoles acutely exposed to 100 μL/L ACE. Acute exposure to DMSO and ETOH had further concentration‐dependent effects on larval morphological traits. Our study suggests that DMSO and ETOH at ≤20 μL/L may be used as solvents in amphibian ecotoxicological studies, but ACE should be limited to ≤50 μL/L in ecotoxicity studies and perhaps much less (≤10 μL/L) in studies with other amphibians, based on a review of existing literature. We emphasize pilot studies when using solvents on acute and chronic ecotoxicity tests, using native amphibians.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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Assessment of sublethal ecotoxicity of solvents on larvae of a model native amphibian (Lithobates pipiens)

Young

Gavel

Gutierrez-Villagómez

et al. 2019

J of Applied Toxicology

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“…Changes in the amount of melanin was found in frogs experimentally exposed to several xenobiotics. In these experiments, the variation in melanin was due to several compounds (reviewed in Oliveira et al 2017), such as polycyclic aromatic hydrocarbons (PAHs; Fanali et al 2018), herbicides (e.g., atrazine and glyphosate; Pérez-Iglesias et al 2019; Bach et al 2019), drugs, and endocrine disrupters (Gregorio et al 2016). Here, we found large amounts of atrazine in the area under agriculture influence (more than 5,000 time the limit accepted by Brazilian legislation, i.e.…”

Section: Discussionmentioning

confidence: 99%

“…< 500 μg/L; Rohr ad McCoy 2010). This substance can change swimming ability, cause malformations, and promote nuclear alterations at higher concentrations in tadpoles (Pérez-Iglesias et al 2019). Atrazine causes oxidative stress in several tissues, which can evolve to function loss.…”

Section: Discussionmentioning

confidence: 99%

Idiosyncratic liver alterations of five frog species to land use changes in the Brazilian Cerrado

Franco‐Belussi

Provete

Borges

et al. 2019

Preprint

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Title Page  Changes in land use promote amphibian declines worldwide  Our study focused on the biosphere, anthroposphere, and hydrosphere  We used method to test for trait-environment relationship  Species responded differently to land use change  Land use change can affect internal morphology and physiology of organisms*Highlights (for review : 3 to 5 bullet points (maximum 85 characters including spaces per bullet point) 1 1 Abstract 2 Changes in land use trigger environmental changes that can lead to decreased 3 biodiversity and species loss. The liver is an essential detoxification organ that reflects 4 systemic physiological responses to environmental changes. Here, we tested whether 5 land use changes influence the amount of substances from the hepatic cellular 6 catabolism and melanomacrophages of five anuran species in the Brazilian Cerrado. We 7 used routine histological and histochemical techniques. We then use recently developed 8 ecological methods to relate functional traits to environmental variables. There was an 9 increase in the amount of melanin in environments with high proportion of agriculture, 10 as well as variation in the amount of lipofuscin and hemosiderin. Therefore, the area of 11 melanomacrophages in the liver and the metabolic products in their cytoplasm can be 12 used as biomarkers of environmental contamination in regions with intense agricultural 13 activities. Our results add a new perspective to the influence of land use changes on 14 environmental health by highlighting the effect of environmental changes on internal 15 morphological aspects of animals.16

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A Double Threat to Amazonian Amphibian Larvae: A Review of Toxic Contaminants and Their Interaction with Global Climate Change

Azambuja,

Kaefer,

Val

et al. 2024

The Future of Amazonian Aquatic Biota

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Biomarkers at different levels of organisation after atrazine formulation (SIPTRAN 500SC®) exposure in Rhinella schineideri (Anura: Bufonidae) Neotropical tadpoles

Cited by 47 publications

References 49 publications

Assessment of sublethal ecotoxicity of solvents on larvae of a model native amphibian (Lithobates pipiens)

Assessment of sublethal ecotoxicity of solvents on larvae of a model native amphibian (Lithobates pipiens)

Idiosyncratic liver alterations of five frog species to land use changes in the Brazilian Cerrado

A Double Threat to Amazonian Amphibian Larvae: A Review of Toxic Contaminants and Their Interaction with Global Climate Change

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