Biomarkers Changes Induced in The Mediterranean Fruit Fly, Ceratitis capitata (Wied.) Irradiated by Sterilising And Substerilising Doses of Gamma Radiation

Abstract

Radiation technology is widely used to produce changes in biosystems. The goal of this work is to determine the variation induced in male parents and F1 Ceratitiis capitata when gamma-irradiated as pupae using substerilising doses (50 and 70 Gy and sterilising dose 90 Gy. Biological studies showed that the percent adult emergence of irradiated pupae decreased as the dose increased and also the percent egg hatchability decreased as the dose increased, also percent pupation and percent emergence of F1 decreased as the dose increased. Comparing DNA (using RAPD-PCR) of untreated adults, adults irradiated with sterilising dose of 90 Gy and parents irradiated with substerile dose (50 and 70 Gy) and their resultant F1. The results revealed that some extra bands appeared and others disappeared, as a result of irradiation. The appearance of extra bands may be due to the repair mechanism of the irradiation damaged DNA. The banding patterns obtained and the dendrograms drawn on the basis of presence and absence of bands revealed that irradiation of parents with 50 and 70 Gy and their F1 are different from the unirradiated pupae It was concluded that the sterile male technique could be used as a benefit tool in controlling Ceratitiis capitata.

Genetic profile of gamma irradiated Locusta migratoria migratorioides: A futuristic eco-friendly control approach

“…The results of the current study on the effects of gamma radiation on adult male and female L. migratoria migrotaria showed that irradiation led to a progressive, signi cant rise in mortality along with an increase in radiation dose. This is in agreement with previous studies which revealed that the mortality of insects was dependent on the dose of gamma radiation (Sayed and Zahran 2017; Kiran et al 2019;Sayed et al 2020). This may be explained by the ndings of Kinipling (1955), who reported that exposure to ionizing radiation causes somatic damage that interferes with the biological functions of the insects and induces genetic dominant fatal mutation with eventual insect sterilization (Ahmed 1992).…”