Biomarkers for Diagnosis and Monitoring of Celiac Disease

Vives-Pi, Marta; Takasawa, Shin; Pujol‐Autonell, Irma; Planas, Raquel; Cabré, Eduard; Ojanguren, Isabel; Montraveta, Montserrat; Santos, Agustín L; Ruiz-Ortiz, Estíbaliz

doi:10.1097/mcg.0b013e31827874e3

Cited by 37 publications

(26 citation statements)

References 42 publications

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“…4D). While some evidence suggests that REG1A is a biomarker of sepsis (Llewelyn et al, 2013) and/or intestinal damage mediated by inflammation (Vives-Pi et al, 2013), increased circulating levels of pancreatic trypsins and lipase are associated with acute pancreatitis (Malfertheiner and Kemmer, 1991), which can be caused by viral infection. Symptoms of acute pancreatitis include severe abdominal pain, and complications may include acute respiratory distress, disseminated intravascular coagulopathy and kidney failure (Agarwal and Pitchumoni, 1993), all of which have been observed in patients with severe EVD (Leligdowicz et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

Multi-platform ’Omics Analysis of Human Ebola Virus Disease Pathogenesis

Eisfeld

Halfmann

Wendler

et al. 2017

Cell Host & Microbe

103

113

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SUMMARY The pathogenesis of Human Ebola virus disease (EVD) is complex. EVD is characterized by high levels of virus replication and dissemination, dysregulated immune responses, extensive virus- and host-mediated tissue damage, and disordered coagulation. To clarify how host responses contribute to EVD pathophysiology, we performed multi-platform ‘omics analysis of peripheral blood mononuclear cells and plasma from EVD patients. Our results indicate that EVD molecular signatures overlap with those of sepsis, imply that pancreatic enzymes contribute to tissue damage in fatal EVD, and suggest that EBOV infection may induce aberrant neutrophils whose activity could explain hallmarks of fatal EVD. Moreover, integrated biomarker prediction identified putative biomarkers from different data platforms that differentiated survivors and fatalities early after infection. This work reveals insight into EVD pathogenesis, suggests an effective approach for biomarker identification, and provides an important community resource for further analysis of human EVD severity.

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Section: Resultsmentioning

confidence: 99%

Multi-platform ’Omics Analysis of Human Ebola Virus Disease Pathogenesis

Eisfeld

Halfmann

Wendler

et al. 2017

Cell Host & Microbe

103

113

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“…The ability to quantify circulating biomarkers in a timely fashion is crucial for monitoring diseases, organ functions, and drug abuse [1–3], so there have been considerable efforts towards developing more convenient and reliable technologies for measuring blood biomarkers [4–6]. Among the technologies, finger-prick has revolutionized our way to monitor plasma glucose levels in patients with diabetics.…”

Section: Introductionmentioning

confidence: 99%

Sample-free quantification of blood biomarkers via laser-treated skin

Wang

Yang

et al. 2015

Biomaterials

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Surface modified microneedle (MN) arrays are being developed to capture circulating biomarkers from the skin, but inefficiency and unreliability of the current method limit its clinical applications. We describe here that illumination of a tiny area of the skin with hemoglobin-preferably absorbent laser increased the amount of circulating biomarkers in the upper dermis by more than 1,000-fold. The hemoglobin-specific light altered the permeability of capillaries leading to extravasation of molecules but not blood cells beneath the skin involved. When specific probe-coated MN arrays were applied into the laser-treated skin, the biomarkers accumulated in the upper dermis were reliably, accurately, and sufficiently captured as early as 15 min of the assay. The maximal binding occurred in one hr in a manner independent of penetration depth or a molecular mass of the biomarker. With anti-fluorescein isothiocyanate (FITC)-MNs, we were able to measure blood concentrations of FITC in mice receiving FITC intravenously. The sensitivity and accuracy were comparable to those attained by fluorescence spectrophotometer. Likewise, MNs containing influenza hemagglutinin (HA) could detect anti-HA antibody in mice or swine receiving influenza vaccines as effectively as standard immunoassays. The novel, minimally invasive approach holds great promise for measurement of multiple biomarkers by a single array for point-of-care diagnosis.

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“…8-15 The early positivity of tTG–ab and EmA is particularly important as they are strongly associated with celiac-type genetics and usually considered as the most specific diagnostic tools in screening of celiac disease. 16-19 …”

Section: Introductionmentioning

confidence: 99%

Early Microbial Markers of Celiac Disease

Viitasalo

Niemi²,

Ashorn³

et al. 2014

Journal of Clinical Gastroenterology

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Background and Goals Seroreactivity against the Saccharomyces cerevisiae (ASCA), Pseudomonas fluorescens-associated sequence (I2) and Bacteroides caccae TonB-linked outer membrane protein (OmpW) has been detected in celiac disease patients with small-bowel mucosal atrophy. Levels of these antibodies decrease during a gluten-free diet, but their functions and time of appearance in celiac disease is not known. We aimed to search for evidence of possible microbial targets of the immune responses in the early stage celiac disease patients who showed normal small-bowel mucosal architecture at the time of the first investigations, but later on a gluten-containing diet developed mucosal atrophy. Methods 44 cases with proven early stage celiac disease and normal mucosal morphology were enrolled. Patients’ sera were tested for celiac disease antibodies against tissue transglutaminase (tTG-ab), endomysium (EmA) and for microbial antibodies against I2, OmpW and ASCA IgG and IgA isotypes in both at the time of diagnosis and while on a gluten-free diet. Results 34 (77%) out of 44 patients with early stage celiac disease had elevated serum antibodies to one or more of the antibodies ASCA, I2 and OmpW. Furthermore, five out of the six cases negative for both tTG-ab and EmA showed positivity for the microbial markers. Seroreactivity to ASCA IgA, ASCA IgG and OmpW decreased significantly during gluten-free diet. Conclusions Seroreactivity to different microbial antigens is evident already in patients with early stage celiac disease. ASCA antibodies seem to be gluten-dependent. The results indicate that microbial targets might have a role in the early development of celiac disease.

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Biomarkers for Diagnosis and Monitoring of Celiac Disease

Cited by 37 publications

References 42 publications

Multi-platform ’Omics Analysis of Human Ebola Virus Disease Pathogenesis

Multi-platform ’Omics Analysis of Human Ebola Virus Disease Pathogenesis

Sample-free quantification of blood biomarkers via laser-treated skin

Early Microbial Markers of Celiac Disease

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