Biomarkers for Periodontal Diseases

Buduneli, Nurcan

doi:10.1007/978-3-030-37317-7_5

Cited by 6 publications

(12 citation statements)

References 103 publications

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“…Both are gnb anaerobic microorganisms that have been linked with periodontal disease progression. Fusobacterium plays a vital role in dental biofilm formation and may explain why smoking has been shown to promote the formation of biofilm [ 39 , 40 ]. This genus, especially the species F. nucleatum, has also been associated with systemic diseases, including CVDs [ 41 ] and uncontrolled type-2 diabetes [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

The effect of cigarette smoking on the oral microbiota in a South African population using subgingival plaque samples

Prince,

Davison,

Davids

et al. 2024

Heliyon

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Section: Discussionmentioning

confidence: 99%

The effect of cigarette smoking on the oral microbiota in a South African population using subgingival plaque samples

Prince,

Davison,

Davids

et al. 2024

Heliyon

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“…The gingival crevicular fluid (GCF) and the peri-implant sulcular fluid (PISF) are tissue fluids that seep through the crevicular and junctional epithelium of periodontal and peri-implant tissues. They have a similar production mechanism due to an increase in the permeability of the vessels underlying the junctional and sulcular epithelium, which might occur due to inflammation, trauma, mechanical stimulation, or presence of highly osmotic substances (bacterial products, plaque) [10,11]. GCF/PISF analysis for inflammation-associated molecules, tissue destruction markers, enzymes, and other proinflammatory mediators has been proposed as an adjunctive diagnostic tool for the early identification of periodontal/peri-implant diseases [10,11].…”

Section: Introductionmentioning

confidence: 99%

“…They have a similar production mechanism due to an increase in the permeability of the vessels underlying the junctional and sulcular epithelium, which might occur due to inflammation, trauma, mechanical stimulation, or presence of highly osmotic substances (bacterial products, plaque) [10,11]. GCF/PISF analysis for inflammation-associated molecules, tissue destruction markers, enzymes, and other proinflammatory mediators has been proposed as an adjunctive diagnostic tool for the early identification of periodontal/peri-implant diseases [10,11]. One of the most investigated biomarkers is represented by the active matrix metalloproteinase-8 (aMMP-8) which is easily detectable in GCF and PISF.…”

Section: Introductionmentioning

confidence: 99%

Relationship between gingival and peri-implant sulcular fluid active matrix metalloproteinase-8 concentration and clinical indices in healthy and diseased conditions

Guarnieri,

Reda,

Zanza

et al. 2024

Exploration of Medicine

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Aim: The study was to evaluate the active matrix metalloproteinase-8 (aMMP-8) concentration in gingival crevicular fluid (GCF) and in peri-implant sulcular fluid (PISF) in healthy and diseased conditions, before and after non-surgical treatment, and to compare it with the various clinical parameters used to estimate the gingival and peri-implant inflammation. Methods: Plaque index/modified PI (PI/mPI), gingival index/simplified GI (GI/sGI), probing depth (PD), bleeding on probing index/modified BOPI (BOPI/mBOPI), radiographic bone loss/radiographic marginal bone loss (rBL/rMBL), and GCF/PISF samples were evaluated, before and 3 months after non-surgical treatment, GCF/PISF samples were analyzed by a chair-side mouth-rinse test (ImplantSafe®) in combination with a digital reader (ORALyzer®). Results: In all groups, aMMP-8 median levels were statistically higher in the PISF than in GCF and they did not change after treatment. Moreover, it was statistically higher in Group 3 (periodontitis/peri-implantitis) compared to the other groups. A positive correlation of the GCF/PISF and aMMP-8 median concentration was seen with increasing PD and BOPI/mBOPI values. A higher covariation of aMMP-8 mean levels in GCF with PD was found when compared to PISF levels. aMMP-8 mean levels in PISF expressed a higher covariation with increasing grades of sGI, rMBL, and BOPI while aMMP-8 GCF concentration established a better covariation with PD and PI. Conclusions: PISF of sites with peri-implant mucositis and peri-implantitis showed higher levels of aMMP-8 compared to sites with gingivitis and periodontitis. Compared to clinical indices, aMMP-8 concentration in GCF/PISF can be a beneficial adjunctive diagnostic tool for early identification and screening of the risk of peri-implant diseases. After non-surgical therapy, PISF aMMP-8 concentration remained mostly unchanged, while the GCF concentration of aMMP-8 significantly decreased.

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“…Results from previous studies support the concept of matrix metalloproteinase‐8 (MMP‐8), also known as neutrophil collagenase or collagenase‐2, as a potential key biomarker responsible for connective tissue destruction or active degeneration of periodontal and peri‐implant disease (Alassy et al, 2019; Al‐Majid et al, 2018; Arias‐Bujanda et al, 2019; Carinci et al, 2019; Gul et al, 2020; Sorsa et al, 2016). MMP‐8 is a major host‐derived collagenolytic proteinase and is regarded as primarily responsible for the irreversible destruction of periodontal and peri‐implant tissues (Buduneli, 2020; Gul et al, 2020; Sorsa et al, 2006). MMP‐8 is responsible for the disintegration and processing of collagens and bioactive inflammatory nonmatrix mediators, not only in various inflammatory and malignant tissue destructive diseases but also in wound healing, immune response and tissue remodeling (Buduneli, 2020; Dejonckheere et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…MMP‐8 is a major host‐derived collagenolytic proteinase and is regarded as primarily responsible for the irreversible destruction of periodontal and peri‐implant tissues (Buduneli, 2020; Gul et al, 2020; Sorsa et al, 2006). MMP‐8 is responsible for the disintegration and processing of collagens and bioactive inflammatory nonmatrix mediators, not only in various inflammatory and malignant tissue destructive diseases but also in wound healing, immune response and tissue remodeling (Buduneli, 2020; Dejonckheere et al, 2011). It can break down almost all the proteinaceous structural components of connective tissues and basement membranes, as well as process distinct bioactive nonmatrix inflammatory immune mediators.…”

Section: Introductionmentioning

confidence: 99%

Active MMP‐8 point‐of‐care (PoC)/chairside enzyme‐test as an adjunctive tool for early and real‐time diagnosis of peri‐implantitis

Lähteenmäki

Tervahartiala

Räisänen

et al. 2022

Clinical & Exp Dental Res

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Objective The aim of this study was to investigate the utility of the active matrix metalloproteinase (aMMP‐8)‐point‐of‐care (PoC) test as a quantitative real‐time chair‐side diagnostic tool for peri‐implant diagnosis, as well as assess the potentially developing and ongoing risk relative to the traditional clinical methods. Background Current peri‐implant and periodontal disease diagnoses rely on clinical and radiological examinations. This case‐control study investigated the applicability of aMMP‐8‐PoC immunotest for quantitative real‐time diagnosis and monitoring of dental implants in health and disease. Methods Sixty‐eight patients visiting a specialist clinic for maintenance following dental implant placement underwent assessment of their peri‐implant health. aMMP‐8‐PoC peri‐implant sulcular fluid (PISF) lateral‐flow immunotests were performed using ImplantSafe® technology quantitated by ORALyzer®. In addition, the PISF samples were analyzed for total MMP‐8, calprotectin, and interleukin (IL)‐6 by enzyme‐linked immunosorbent assays (ELISA), aMMP‐8 by western immunoblot, and MMP‐2 and MMP‐9 by gelatin zymography. Results The aMMP‐8‐PoC test promptly recorded and reflected peri‐implant disease, differentiating it clearly from health. X‐ray findings (bone loss > 2 mm), peri‐implant pocket depth ≥ 3 mm, and bleeding on probing were significantly more prevalent among implants positive for the aMMP‐8‐PoC test. aMMP‐8/ORALyzer analysis was more precise in recording disease than total MMP‐8, calprotectin, IL‐6, MMP‐2, and MMP‐9. Conclusions The aMMP‐8‐PoC test can be conveniently implemented to alert for and detect active collagenolysis affecting peri‐implant tissues, both in the early and advanced stages of the disease. Active and fragmented MMP‐8 exhibits a strong and significant association with peri‐implantitis as compared to total MMP‐8 and other biomarkers and can be utilized as the POC/chairside biomarker of choice in the new classification of peri‐implantitis.

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Biomarkers for Periodontal Diseases

Cited by 6 publications

References 103 publications

The effect of cigarette smoking on the oral microbiota in a South African population using subgingival plaque samples

The effect of cigarette smoking on the oral microbiota in a South African population using subgingival plaque samples

Relationship between gingival and peri-implant sulcular fluid active matrix metalloproteinase-8 concentration and clinical indices in healthy and diseased conditions

Active MMP‐8 point‐of‐care (PoC)/chairside enzyme‐test as an adjunctive tool for early and real‐time diagnosis of peri‐implantitis

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