Biomarkers in Endometriosis

Nisenblat, Vicki; Hull, M. Louise

doi:10.1007/978-3-319-71138-6_16

Cited by 1 publication

(1 citation statement)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Currently, to reliably diagnose endometriosis, laparoscopy with inspection of the abdominal cavity and histological confirmation of the suspicious lesion must be performed. While alternative methods such as medical imaging, genetic tests, or biomarkers are aimed at alleviating invasive diagnosis, none of them can adequately substitute for surgery at present [ 23 , 24 , 25 , 26 , 27 ]. Recent studies have identified a promising tissular biomarker called B-cell lymphoma 6 (BCL6), which could be used to diagnose women affected by endometriosis [ 1 , 2 , 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Morphological and Digital-Assisted Analysis for BCL6 Endometrial Expression in Women with Endometriosis

Squatrito

Blacher

Henry

et al. 2022

JCM

View full text Add to dashboard Cite

BCL6 (B-cell lymphoma 6) is a proto-oncogene and transcriptional repressor initially described as being involved in B-cell lymphoma. Recently, this factor has been identified as a promising tissue biomarker which could be used to diagnose women affected by endometriosis. Previous studies used HSCORE for BCL6 staining quantification in the endometrium. However, this semi-quantitative technique of analysis has some limitations, including a lack of objectivity, robustness, and reproducibility that may lead to intra- and inter-observer variability. Our main goal was to develop an original computer-assisted method to quantify BCL6 staining from whole-slide images reliably. In order to test the efficiency of our new digital method of quantification, we compared endometrial BCL6 expression between fertile and infertile women without or with different stages of endometriosis by using the widely used HSCORE analysis and our new automatic digital image analysis. We find a higher expression of BCL6 in the endometrium of infertile women with endometriosis and women with stage IV endometriosis. Furthermore, we demonstrate a significant correlation between the two types of independent measurements, indicating the robustness of results and also the reliability of our computer-assisted method for BCL6 quantification. In conclusion, our work, by using this original computer-assisted method, enables BCL6 quantification more objectively, reliably, robustly, and promptly compared to HSCORE analysis.

show abstract