Biomarkers of air pollution exposure—A study of policemen in Prague

Topinka, Jan; Sevastyanova, Oksana; Binková, Blanka; Chvátalová, Irena; Milcová, Alena; Lnenickova, Zdena; Nováková, Zuzana; Solanský, Ivo; Šrám, Radìm J.

doi:10.1016/j.mrfmmm.2007.02.032

Cited by 35 publications

(33 citation statements)

References 38 publications

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“…In subjects exposed in industry to higher doses of PAHs, the relationship between BPDE-DNA adducts and GSTM1 was more evident (11,12,21,22). Further, in parallel with GSTM1, the BaP derivates may also be conjugated by UDP glucuronosyltransferases to corresponding glucuronides.…”

Section: Resultsmentioning

confidence: 99%

“…It is apparent that the activity of GSTM1 influences the level of BPDE and hence also the level of BPDE-DNA adducts. However, the results of studies focused on relationships between the GSTM1 activity and the level of BPDE-DNA adducts, are still inconsistent (11)(12)(13). Moreover, only a little is known about genotoxic and mutagenic risks associated to GSTM1*0/*1 heterozygosity (10).…”

Section: Introductionmentioning

confidence: 99%

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Droplet Digital PCR Analysis of GSTM1 Deletion Polymorphism in Psoriatic Subjects Treated with Goeckerman Therapy

Beránek

Fiala

Kremláček

et al. 2016

Acta Med. (Hradec Kralove, Czech Repub.)

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Summary: Goeckerman therapy (GT) represents an effective treatment of psoriasis including a combination of pharmaceutical grade crude coal tar (CCT) and ultraviolet irradiation (UV-R). Coal tar contains a mixture of polycyclic aromatic hydrocarbons. The best known carcinogenic polyaromate -benzo [a]pyrene is metabolized into a highly reactive benzo [a] pyrene-7,8-diol-9,10-epoxide (BPDE). Glutathione S-transferase M1 (GSTM1) catalyses the conjugation of drugs, toxins and products of oxidative stress with glutathione. The aim of the study is to found possible associations between GSTM1 genotypes and the level of BPDE-DNA adducts in 46 psoriatic patients treated with GT. For genotyping, droplet digital PCR was applied. The GSTM1 copy number was normalized to β-globin reference gene. In five GSTM1*1/*1 subjects, the GSTM1 to β-globin ratio moved from 0.99 to 1.03 with a median of 1.01. GSTM1*0/*1 heterozygotes (n = 20) contained only one GSTM1 function allele which conditioned the ratio 0.47-0.53 (median 0.50). GSTM1*0/*0 individuals (n = 21) showed no amplification of the null variants because of the large deletion in GSTM1. BPDE-DNA concentrations ranged from 1.8 to 66.3 ng/µg with a median of 12.3 ng/µg. GSTM1*0/*0 and GSTM1*0/*1 genotypes showed non-significantly higher concentrations of BPDE-DNA adducts than the GSTM1*1/*1 one (12.3 and 12.4 vs 7.8 ng/µg). The non-significant relationship between BPDE-DNA adducts and GSTM1 genotypes in psoriatic patients could be associated with relatively low doses of CCT and short-term UV-R exposures used in GT.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Droplet Digital PCR Analysis of GSTM1 Deletion Polymorphism in Psoriatic Subjects Treated with Goeckerman Therapy

Beránek

Fiala

Kremláček

et al. 2016

Acta Med. (Hradec Kralove, Czech Repub.)

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“…Although DNA adducts are targets of multiple DNA repair enzymes, some modifications may persist and produce several undesirable effects, such as nucleotide substitutions, leading to DNA mutations and synthesis of altered protein products (19). Many environmental air pollutants (20), tobacco smoke (20)(21)(22) and PAHs from traffic exhaust (23,24) or from occupational exposure (21) have shown a positive correlation with DNA adduct formation in exposed populations. For further information, see reference (25).…”

Section: Dna Adductsmentioning

confidence: 99%

Molecular biomarkers to assess health risks due to environmental contaminants exposure

Poblete-Naredo

Albores

2016

biomedica

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<p>Los biomarcadores, o bioindicadores, son herramientas métricas que, al compararse con los valores de referencia, permiten evaluar los riesgos y generar información objetiva que ayude a las autoridades a planificar estrategias efectivas, solucionar problemas de salud o ambientales y utilizar los recursos asignados de manera eficiente.<br />La evaluación de riesgos en salud es un ejercicio multidisciplinario y la biología molecular contribuye enormemente a estos estudios, dado que el genoma, el transcriptoma, el proteoma y el metaboloma pueden verse afectados por xenobióticos, lo que causa cambios cuya medición resulta útil en la adopción de decisiones. Dichos cambios pueden variar por la carga genética de cada individuo y su distribución polimorfa en las poblaciones expuestas se convierte en un factor esencial para una adecuada interpretación de resultados.<br />Por lo tanto, el objetivo del presente artículo fue hacer una revisión de los diferentes biomarcadores moleculares aplicables a la evaluación de riesgos para la salud provenientes de los contaminantes ambientales.</p>

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“…However, the association between vitamin levels as measured in plasma on several measures of DNA damage and oxidation has been rarely performed. A study on 109 non smoking policemen conducted in Prague, reported a weak negative association between plasma vitamin A levels and both Total DNA adducts and B[a]P-like DNA adducts (9). Another study from the same group on a small population of 50 bus drivers showed that vitamin E levels were positively correlated with markers of oxidative stress (10).…”

Section: Introductionmentioning

confidence: 99%

Effect of vitamin levels on biomarkers of exposure and oxidative damage—The EXPAH study

Šrám

Farmer

Singh

et al. 2009

Mutation Research/Genetic Toxicology and Environmental Mutagene

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DNA adducts are markers of carcinogen exposure and of their biological effect; they have been shown to be related to mutagenesis, and therefore they could be a predictive biomarker of human cancer.. The objective of this study was to assess if there is a relationship between vitamins A, C, and E, which are known to play a significant role as free radical scavengers and antioxidant agents, and biomarkers of genotoxicity and oxidative stress.Three hundred and fifty-six subjects from Czech Republic, Slovak Republic and Bulgaria, who completed a questionnaire on dietary information and had a measurement of plasma A, C, E vitamins, DNA adduct levels (benzo[a]pyrene (B[a]P) and bulky (DNA-Tot) DNA adducts) and oxidative damage (cyclic pyrimidopurinone N−1,N2 malondialdehyde -2 deoxyguanosine (M1dG) and 8-oxo-7,8-dihydro2_deoxyguanosine (8-oxodG)) were analyzed. A significant inverse correlation was observed between plasma vitamin levels and both benzo[a]pyrene (B[a]P) and bulky DNA adducts. Vitamin A was also significantly inversely correlated with M1dG, a marker of oxidative damage. The associations were stronger in non smokers than in smokers.

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Biomarkers of air pollution exposure—A study of policemen in Prague

Cited by 35 publications

References 38 publications

Droplet Digital PCR Analysis of GSTM1 Deletion Polymorphism in Psoriatic Subjects Treated with Goeckerman Therapy

Droplet Digital PCR Analysis of GSTM1 Deletion Polymorphism in Psoriatic Subjects Treated with Goeckerman Therapy

Molecular biomarkers to assess health risks due to environmental contaminants exposure

Effect of vitamin levels on biomarkers of exposure and oxidative damage—The EXPAH study

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