Biomarkers of Bacillus subtilis total lipids FAME profile under various temperatures and growth phases

Ibragimova, M. Y.; Салафутдинов, И. И.; Şahın, Fikrettin; Жданов, Р. И.

doi:10.1134/s1607672912020135

Cited by 5 publications

(2 citation statements)

References 13 publications

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“…For decades, the fatty acid profiles have been used for microbial identification. It has been proved that the fatty acid profiles are unique from one species to another (Tighe et al, 2000;Ivanova et al, 2001;Slabbinck et al, 2010;Li et al, 2011) and vary under different growth conditions (Hamamoto et al, 1994;Day & Oliver, 2004;Chiang et al, 2005;Fouchard et al, 2005;Ibragimova et al, 2012). The major fatty acids of the Vibrio strains, are observed most often, are hexadecenoic (16:1), hexadecanoic (16:0), and octadecenoic (18:1) acids (Urdaci et al, 1990;Carballeira et al, 1995).…”

Section: Cellular Fatty Acid Profilesmentioning

confidence: 99%

Visualized analysis of cellular fatty acid profiles ofVibrio parahaemolyticusstrains under cold stress

Jia

Chen

Jiang

et al. 2014

FEMS Microbiol Lett

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Vibrio parahaemolyticus is a common foodborne bacterial pathogen, which survives in cold environments and is sometimes difficult to culture. Fatty acid analysis under cold stress was conducted for several V. parahaemolyticus strains using gas chromatography/mass spectrometry, and the results were compared with those of the controls. All the fatty acid profiles obtained were visualized by multidimensional scaling (MDS) and self-organized map (SOM). It was observed that the fatty acid profiles of V. parahaemolyticus substantially changed under cold stress. The percentage of methyl palmitate remarkably decreased and that of methyl palmitoleate (except for two strains) and methyl oleate increased. These findings demonstrate the role of fatty acids in cold stress. The changes in the fatty acid profiles illustrated by MDS and SOM could differentiate strains under cold stress from the controls and can potentially lead to a method of detecting injured cold-stressed V. parahaemolyticus.

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Section: Cellular Fatty Acid Profilesmentioning

confidence: 99%

Visualized analysis of cellular fatty acid profiles ofVibrio parahaemolyticusstrains under cold stress

Jia

Chen

Jiang

et al. 2014

FEMS Microbiol Lett

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“…Based on our results, we strongly recommend the use of pfaA-KS PCR screening when analyzing a large number of samples in order to minimize the effort required for GC analysis. This molecular approach has the advantage of being independent of bacterial growth, temperature, and media composition, which are the main sources of variations in the analysis of microbial fatty acid profiles [45][46][47][48].…”

Section: Discussionmentioning

confidence: 99%

Novel Vibrio spp. Strains Producing Omega-3 Fatty Acids Isolated from Coastal Seawater

et al. 2020

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Omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs), such as eicosapentaenoic acid (EPA) (20:5n-3) and docosahexaenoic acid (DHA) (22:6n-3), are considered essential for human health. Microorganisms are the primary producers of omega-3 fatty acids in marine ecosystems, representing a sustainable source of these lipids, as an alternative to the fish industry. Some marine bacteria can produce LC-PUFAs de novo via the Polyunsaturated Fatty Acid (Pfa) synthase/ Polyketide Synthase (PKS) pathway, which does not require desaturation and elongation of saturated fatty acids. Cultivation-independent surveys have revealed that the diversity of microorganisms harboring a molecular marker of the pfa gene cluster (i.e., pfaA-KS domain) is high and their potential distribution in marine systems is widespread, from surface seawater to sediments. However, the isolation of PUFA producers from marine waters has been typically restricted to deep or cold environments. Here, we report a phenotypic and genotypic screening for the identification of omega-3 fatty acid producers in free-living bacterial strains isolated from 5, 500, and 1000 m deep coastal seawater from the Bay of Biscay (Spain). We further measured EPA production in pelagic Vibrio sp. strains collected at the three different depths. Vibrio sp. EPA-producers and non-producers were simultaneously isolated from the same water samples and shared a high percentage of identity in their 16S rRNA genes, supporting the view that the pfa gene cluster can be horizontally transferred. Within a cluster of EPA-producers, we found intraspecific variation in the levels of EPA synthesis for isolates harboring different genetic variants of the pfaA-KS domain. The maximum production of EPA was found in a Vibrio sp. strain isolated from a 1000 m depth (average 4.29% ± 1.07 of total fatty acids at 10 °C, without any optimization of culturing conditions).

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Biodegradation of phenanthrene by piezotolerant Bacillus subtilis EB1 and genomic insights for bioremediation

Kumar

Manisha

Rajan

et al. 2023

Marine Pollution Bulletin

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Biomarkers of Bacillus subtilis total lipids FAME profile under various temperatures and growth phases

Cited by 5 publications

References 13 publications

Visualized analysis of cellular fatty acid profiles ofVibrio parahaemolyticusstrains under cold stress

Visualized analysis of cellular fatty acid profiles ofVibrio parahaemolyticusstrains under cold stress

Novel Vibrio spp. Strains Producing Omega-3 Fatty Acids Isolated from Coastal Seawater

Biodegradation of phenanthrene by piezotolerant Bacillus subtilis EB1 and genomic insights for bioremediation

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