Biomarkers of exposure to endogenous oxidative and aldehyde stress

Bruce, W. R.; Lee, Owen; Liu, Zhen; Marcon, Norman E.; Minkin, Salomon; O’Brien, Peter J.

doi:10.3109/1354750x.2011.580369

Cited by 6 publications

(2 citation statements)

References 41 publications

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“…Recent attempts to find association between Advanced Glycation Products (AGEs) and colorectal cancer in more than 1000 patients did not find any significant correlations [28,29]. Many such studies applying metabolomics tools are in progress.…”

Section: What Are Endogenous Toxins Of Cancer?mentioning

confidence: 99%

Untitled

2016

JAPLR

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Section: What Are Endogenous Toxins Of Cancer?mentioning

confidence: 99%

Untitled

2016

JAPLR

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“…Environmental aldehydes arise from a number of sources, as they (1) are formed in water as disinfection byproducts of ozonation (2) are used as food additives, and (3) result from pyrolysis of organic matter such as fuel, wood, and tobacco. − In addition, the body generates aldehydes endogenously from the reaction of free radicals with cell membrane lipids.…”

Section: Introductionmentioning

confidence: 99%

Quantification of 19 Aldehydes in Human Serum by Headspace SPME/GC/High-Resolution Mass Spectrometry

Silva

Hile

Capella

et al. 2018

Environ. Sci. Technol.

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Sources of human aldehyde exposure include food additives, combustion of organic matter (tobacco smoke), water disinfection byproducts via ozonation, and endogenous processes. Aldehydes are potentially carcinogenic and mutagenic, and chronic human aldehyde exposure has raised concerns about potential deleterious health effects. To aid investigations of human aldehyde exposure, we developed a novel method to measure 19 aldehydes released from Schiff base protein adducts in serum using controlled acid hydrolysis, solid-phase microextraction (SPME), gas chromatography (GC), and high-resolution mass spectrometry (HRMS). Aldehydes are released from Schiff base protein adducts through acid hydrolysis, and are quantified in trace amounts (μg/L) using stable isotope dilution. Detection limits range from 0.1 to 50 μg/L, with calibration curves spanning 3 orders of magnitude. The analysis of fortified quality control material over a three-month period showed excellent precision and long-term stability (3-22% CV) for samples stored at -70 °C. The intraday precision is also excellent (CV, 1-10%). The method accuracy ranges from 89 to 108% for all measured aldehydes, except acrolein and crotonaldehyde, two aldehydes present in tobacco smoke; their analysis by this method is not considered robust due in part to their reactivity in vivo. However, results strongly suggest that propanal, butanal, isobutanal, and isopentanal levels in smokers are higher than levels in nonsmokers, and thus may be useful as biomarkers of tobacco smoke exposure. This method will facilitate large epidemiological studies involving aldehyde biomonitoring to examine nonoccupational environmental exposures.

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