Biomarkers of metabolism disturbance in bivalve molluscs induced by environmental pollution with processed by-products of oil

Abstract

Processed by-products of oil are the most common pollutants in all river and sea water. The increase in oxidative stress in bivalve molluscs was studied in both tissues of the hepatopancreas and the gill. The model for artificial treatment with processed by-products of oil was performed in a laboratory experiment with the river mollusc Dreissena polymorpha Pallas, 1771. The exposure of the molluscs over 28 days to mazut 50 mg/l induced significant increase of both final product of lipid peroxidation (LPO) and antioxidant enzime activity. A significant increase in LPO was observed in the hepatopancreas and gill of D. polymorpha treated with mazut compared to the control group. Antioxidant enzyme activity of cartalase, supeoxide dismutase, glutatione reductase and glutatione-S-transferase showed a greater increase (by almost 1.5 times) in the hepatopancreas than in the gill of D. polymorpha. A similar LPO growth and modulation of antioxidant enzyme activity were determined in the hepatopancreas and gill of the mussel Mytilus galloprovincialis Lamarck, 1879 collected in an area polluted with resins, hydrocarbons and asphaltenes, Donuzlav lake in the Kerch gulf. Varied cellular reactivation of the antioxidant enzyme system in the hepatopancreas rather than the gill was observed in both kinds of mollusc Dreissena and Mytilus. The obtained results are evidence of the higher sensitivity of the hepatopancreas cells of bivalve molluscs to organic pollutants compared to the gill cells.

Molecular mechanisms of aluminium ions neurotoxicity in brain cells of fish from various pelagic areas

“…The nature of the glial response depends largely on both the nature of the damaging factors and the duration of the exposure: mechanical damage to the central nervous system induces rapidly increasing astrogliosis (Suzuki et al, 2012), and processed by-products of oil and aluminum ions cause a slower astroglial reaction (Nedzvetskii et al, 2006;Sukharenko et al, 2017). At the same time, various factors can cause similar astroglial reactivity.…”

Phototropic response features for different systematic groups of mesoplankton under adverse environmental conditions

“…Over the past two decades, quite a few studies have been devoted to water biotesting techniques based on the analysis of various features of aquatic organisms, namely survival registration (OECD, 2013(OECD, , 2019, reproducibility, offspring quality, morphological parameter changes (OECD, 2013), physiological functions, and behavioral responses (Lechelt et al, 2000;Morgalev et al, 2015;Nikitin, 2014;Wang et al, 2019). The most promising are the methods of water biomonitoring using behavioral responses of local species of hydrobionts, and primarily bivalves (Sukharenko et al, 2017), branchiopoda (Carreño-León et al, 2014) and copepoda (Lechelt et al, 2000;Pan et al, 2015Pan et al, , 2017Ren et al, 2017), and fish (Ren et al, 2016). Daphnia are particularly interesting with regard to these methods.…”

“…This will inevitably cause changes in the quality of the ecosystem and potential disastrous reduction in the number of aboriginal species. Therefore, today there is an urgent need for prompt early control of pollution of natural water areas by microconcentration of pollutants (Sukharenko et al., 2017). Besides, early detection of pollutants is critical in hazardous areas such as nuclear stations, oil platforms, and gas pipelines.…”

Biomarkers of behavioral reactions of mussels in automated biomonitoring systems under conditions of aquatic environment pollution by drill cuttings and oil hydrocarbons

“…1в), что является показателем развития стрессовой ситуации [6,7,17]. Установленные в наших экспериментах закономерности изменений поведенческих реакций моллюсков в полной мере согласуются с выводами исследователей о том, что при воздействиях нефтяного загрязнения невысокой интенсивности отмечается наиболее выраженная в разной степени перестройка ферментных систем, направленная на снижение повреждающего воздействия токсикантов: лизосомальной активности [10], антиоксидантного комплекса [21,22], ферментов гликолитического фосфорилирования, белкового синтеза [9].…”

“…Это может быть свидетельством начала адаптивных физиоло-го-биохимических перестроек метаболизма как при хронических воздействиях невысокой интенсивности, так и начала глубоких адаптивных физиолого-биохимических изменений метаболизма в результате длительного воздействия токсиканта [9,10]. Наиболее значительные повреждающие изменения метаболизма моллюсков происходят при повторяющихся частых разливах нефти невысокой концентрации [10,[21][22][23]. Очевидно, что именно эти изменения метаболизма находит свое отражение в глубоких изменениях поведенческих реакций у моллюсков, сопровождающихся разрушением суточного ритма, нарушением процессов фильтрации и питания, гаметогенеза [14,21].…”