Biomarkers of oxidative stress and acetylcholinesterase activity in the blood of grass snake (Natrix natrix L.) during prehibernation and posthibernation periods

Gavrić, Jelena P.; Prokić, Marko D.; Despotović, Svetlana G.; Gavrilović, Branka R.; Radovanović, Tijana B.; Borković-Mitić, Slavica; Pavlović, Slađan; Saičić, Zorica S.

doi:10.1590/s1516-8913201500308

Cited by 11 publications

(1 citation statement)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reactive oxygen species (ROS), which are produced throughout intracellular metabolism, have physiological effects at low concentrations. However, if ROS are overproduced after environmental contaminations, they may have deleterious effects on cell structures, proteins, DNA, and lipids (see Gavrić et al for a review). As a result, antioxidant enzymes, especially SOD and CAT, are activated to detoxify ROS and counteract their deleterious effects .…”

Section: Discussionmentioning

confidence: 99%

Oxidative stress induced by glyphosate-based herbicide on freshwater turtles

Héritier

Duval

Galinier

et al. 2017

Environmental Toxicology and Chemistry

View full text Add to dashboard Cite

Freshwater ecosystems face very strong anthropogenic pressures, among which overexploitation, habitat degradation, flow modification, species invasion, and water pollution lead to growing threats on biodiversity. Urbanization through wastewater treatment, industry through the release of inorganic and organic chemicals, and agriculture through the use of pesticides and herbicides are the main factors involved in water pollution. In France, more precisely in the Pyrénées-Orientales department, the poor quality of the watercourses is attributable overall to the use of glyphosate-based herbicides in agricultural activities. Because these chemicals can impact individuals, populations, and biodiversity, we investigated, under experimental conditions, the physiological response of animals facing abiotic contaminants. We selected as a model, juveniles of the freshwater turtle Trachemys scripta elegans. We measured the gene expression and activity of the catalase and superoxide dismutase enzymes as well as the levels of lipid peroxidation, which are all oxidative stress biomarkers, in turtles challenged with high concentrations of glyphosate-based herbicides, on the one hand, and with degraded waters collected from a local watercourse, on the other. We also measured the acetylcholinesterase activity across the same animals. We showed through variations in gene expression and enzyme activity that a glyphosate commercial formulation induced a stress in turtles. A similar outcome was obtained when turtles faced degraded waters. The results indicated that the poor quality of regional waters could be a real threat for animal health. Because turtles are globally less sensitive to contaminants than amphibians, which are lacking in the degraded waters of the Pyrénées-Orientales department, they could constitute an excellent model to follow the evolution of water quality through the study of oxidative stress biomarkers. Environ Toxicol Chem 2017;36:3343-3350. © 2017 SETAC.

show abstract

Section: Discussionmentioning

confidence: 99%