Biomarkers of oxidative stress study

Kadiiska, Maria B.; Gladen, Beth C.; Baird, Donna D.; Graham, Louella W.; Parker, Carol E.; Ames, Bruce N.; Basu, Samar; FitzGerald, Garret A.; Lawson, John A.; Marnett, Lawrence J.; Morrow, Jason D.; Murray, Dennis; Plastaras, John P.; Roberts, L. Jackson; Rokach, Joshua; Shigenaga, Mark K.; Sun, Jing; Walter, Patrick; Tomer, Kenneth B.; Barrett, J. Carl; Mason, Ronald P.

doi:10.1016/j.freeradbiomed.2004.10.024

Cited by 154 publications

(60 citation statements)

References 36 publications

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“…With the historical evidence and recent reinforcement of the complex generation mechanism of F 2 -isoprostanes, especially 8-iso-PGF 2α [25], [35], [36], [37], [38], [39], [40], [41], [42], [43], [44], simple explanations based on historical dogma for the mechanism behind the effect or non-effect of specific strategies should be made with caution. As has been pointed out before [45], [46], [47], in addition to affecting formation by scavenging free radicals, 8-iso-PGF 2α levels can be manipulated by changes in metabolism, excretion rates, substrate availability, redox-environment, and enzymatic activities of prostaglandin H synthase (PGHS).…”

Section: Discussionmentioning

confidence: 99%

“…There are numerous biomarkers that could be utilized to quantitate the levels of oxidative stress. Through the multi-model, multi-laboratory Biomarkers of Oxidative Stress study, many biomarkers were compared, with the most indicative marker being the F 2 -isoprostanes and specifically 8-iso-prostaglandin F 2α (8-iso-PGF 2α ) [21], [22], [23], [24], [25]. Since no other biomarker is recommended, this meta-analysis will focus solely on 8-iso-PGF 2α as the biomarker.…”

Section: Introductionmentioning

confidence: 99%

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Strategies to decrease oxidative stress biomarker levels in human medical conditions: A meta-analysis on 8-iso-prostaglandin F2α

Erve

2018

Redox Biology

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The widespread detection of elevated oxidative stress levels in many medical conditions has led to numerous efforts to design interventions to reduce its effects. Efforts have been wide-ranging, from dietary changes to administration of antioxidants, supplements, e.g., omega-3-fatty acids, and many medications. However, there is still no systemic assessment of the efficacy of treatments for oxidative stress reduction across a variety of medical conditions.The goal of this meta-analysis is, by combining multiple studies, to quantitate the change in the levels of the popular oxidative stress biomarker 8-iso-prostaglandin F2α (8-iso-PGF2α) after a variety of treatment strategies in human populations.Nearly 350 unique publications with 180 distinct strategies were included in the analysis. For each strategy, the difference between pre- or placebo and post-treatment levels calculated using Hedges’ g value of effect. In general, administration of antibiotics, antihyperlipidemic agents, or changes in lifestyle (g = − 0.63, − 0.54, and 0.56) had the largest effect. Administration of supplements, antioxidants, or changes in diet (g = − 0.09, − 0.28, − 0.12) had small quantitative effects. To fully interpret the effectiveness of these treatments, comparisons to the increase in g value for each medical condition is required. For example, antioxidants in populations with coronary artery disease (CAD) reduce the 8-iso-PGF2α levels by g = − 0.34 ± 0.1, which is quantitatively considered a small effect. However, CAD populations, in comparison to healthy populations, have an increase in 8-iso-PGF2α levels by g = 0.38 ± 0.04; therefore, the overall reduction of 8-iso-PGF2α levels is ≈ 90% by this treatment in this specific medical condition.In conclusion, 8-iso-PGF2α levels can be reduced not only by antioxidants but by many other strategies. Not all strategies are equally effective at reducing 8-iso-PGF2α levels. In addition, the effectiveness of any strategy can be assessed only in relation to the medical condition investigated.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Strategies to decrease oxidative stress biomarker levels in human medical conditions: A meta-analysis on 8-iso-prostaglandin F2α

Erve

2018

Redox Biology

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“…As MDA is probably a real-time marker of oxidative injury [28, 29] and indicative of current liver iron concentration, MDA was studied as a marker of tissue injury and oxidative stress. In our study, plasma MDA was significantly increased in thalassaemia, compared with control patients; however, after vitamin E treatment, those values bordered normal values.…”

Section: Discussionmentioning

confidence: 99%

Vitamin E Supplementation Reduces Oxidative Stress in Beta Thalassaemia Intermedia

Pfeifer¹,

Degasperi²,

Almeida³

et al. 2008

Acta Haematol

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Objective: The aim of this investigation was to study the effect of vitamin E treatment in oxidative stress of red and white cells of β-thalassaemia intermedia patients. Methods: Nine patients undergoing occasional transfusions (5 females/4 males), median age 39 years (range 15–74), were recruited for oral daily administration of 400 IU vitamin E for 3 months. Twenty-seven milliliters of peripheral blood was obtained before and after 3 months of treatment, and 3 months after treatment completion. In the case of transfused patients (n = 4), blood was obtained at least 30 days after transfusion. Reactive oxygen species (ROS) was measured by flow cytometry; red blood cell (RBC) reduced glutathione (GSH) was measured by dinitrothiocyanobenzene reduction, serum malondialdehyde was measured in terms of thiobarbituric acid-reactive substances (TBARS), and α-haemoglobin-stabilizing protein (AHSP) mRNA expression was measured by real-time polymerase chain reaction of reticulocyte RNA extracts. Results: β-Thalassaemia patients presented basal levels of RBC ROS, GSH and serum TBARS statistically different compared with healthy controls. However, after vitamin E administration, patients presented a significant reduction in erythrocyte RBC ROS and serum TBARS levels. In parallel, red cell GSH was significantly increased after treatment. Peripheral mononuclear cells and T lymphocytes also demonstrated a reduction in ROS production. Therefore, after treatment, no significant differences were detected comparing patients and normal controls. Three months after treatment completion, all measurements showed a tendency of returning to basal values. A significant reduction in reticulocyte number was observed after vitamin E treatment. Vitamin E treatment did not modify levels of haemoglobin or AHSP mRNA expression. Conclusion: Although vitamin E is not capable of reducing anaemia in these patients, it could be useful for reducing oxidative damage in other target organs of β-thalassaemic patients. Finally, this is the first study to analyse the effects of vitamin E on ROS production in red and white blood cells and AHSP mRNA expression.

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“…The diversity of oxidative species implies that the choice of biomarkers, which can be universally applied to characterise systemic oxidative stress in living organisms, constitutes a major challenge. Indeed, pioneering comprehensive comparative studies addressing this issue have recently been initiated in an animal model of oxidative stress by the US National Institute of Environmental Health Sciences [9,10].…”

Section: Introductionmentioning

confidence: 99%

Distinct profiles of systemic biomarkers of oxidative stress in chronic human pathologies: Cardiovascular, psychiatric, neurodegenerative, rheumatic, infectious, neoplasmic and endocrinological diseases

Brack¹,

Brack²,

Ménézo³

et al. 2013

ABB

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Oxidative stress is involved in chronic and acute pathologies: cardiovascular, neurodegenerative, neoplastic, inflammatory and infectious diseases. Clinical trials focused on prevention of cardiovascular and neoplastic diseases involving antioxidant supplementation have however provided predominantly negative obserations in large-scale studies. Screening of patient cohorts to assess baseline oxidative stress on the basis of a biomarker profile is decisive but lacking. For the first time, we evaluated the level of oxidative stress, testing more than 10 established biomarkers, in a comprehensive initial survey of 617 patients displaying chronic human pathologies. Multiple diseasespecific abnormalities were identified in plasma, whole blood and/or urine. This is the case for vitamins and oligo elements, vitamin C, vitamin E, β-carotene, selenium, zinc and copper; endogenous antioxidants such as reduced and oxidised glutathione, thiols, urate, and glutathione peroxidase activity, and a biomarker of oxidative DNA damage (8-hydroxy-2'-deoxyguanosine). The distinct biomarker profiles suggest the involvment of multiple forms of oxidative insults which are in some way partially specific to each pathological condition. This finding is in favor of the determination of an integrated score to combine contributions of distinct biomarkers, in order to screen patients presenting elevated levels of oxidative stress.

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Biomarkers of oxidative stress study

Cited by 154 publications

References 36 publications

Strategies to decrease oxidative stress biomarker levels in human medical conditions: A meta-analysis on 8-iso-prostaglandin F2α

Strategies to decrease oxidative stress biomarker levels in human medical conditions: A meta-analysis on 8-iso-prostaglandin F2α

Vitamin E Supplementation Reduces Oxidative Stress in Beta Thalassaemia Intermedia

Distinct profiles of systemic biomarkers of oxidative stress in chronic human pathologies: Cardiovascular, psychiatric, neurodegenerative, rheumatic, infectious, neoplasmic and endocrinological diseases

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