Biomass and Lipid Productivities of Cyanobacteria- Leptolyngbya foveolarum HNBGU001

Singh, Preeti; Kumar, Dhananjay

doi:10.1007/s12155-020-10170-3

Cited by 23 publications

(5 citation statements)

References 46 publications

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“…The decrease in lipid contents of Cyanothece cells at high pH agreed with previous observations on the cyanobacterium Leptolyngbya foveolarum (Singh and Kumar 2021 ). Unfortunately, Cyanothece sp.…”

Section: Discussionsupporting

confidence: 91%

Enhancement of biomass productivity and biochemical composition of alkaliphilic microalgae by mixotrophic cultivation using cheese whey for biofuel production

Youssef,

Gomaa,

Mohamed

et al. 2024

Environ Sci Pollut Res

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The growth of microalgae under alkaline conditions ensures an ample supply of CO2 from the atmosphere, with a low risk of crashing due to contamination and predators. The present study investigated the mixotrophic cultivation of two alkaliphilic microalgae (Tetradesmus obliquus and Cyanothece sp.) using cheese whey as an organic carbon source. The variation in cheese whey concentration (0.5–4.5% (v/v)), culture pH (7–11), and NaNO3 concentrations (0–2 gL−1) was evaluated using central composite design in response to biomass productivity and the contents of lipids, total proteins, and soluble carbohydrates. Both investigated microalgae effectively utilized cheese whey as an organic carbon source. The optimum conditions for simultaneously maximizing biomass and lipid productivity in T. obliquus were 3.5% (v/v) whey, pH 10.0, and 0.5 g L−1 NaNO3. Under these conditions, the biomass, lipid, soluble carbohydrate, and protein productivities were 48.69, 20.64, 7.02, and 10.97 mg L−1 day−1, respectively. Meanwhile, Cyanothece produced 52.78, 11.42, 4.31, and 7.89 mg L−1 day−1 of biomass, lipid, carbohydrate, and protein, respectively, at 4.5% (v/v) whey, pH 9.0, and 1.0 g L−1 NaNO3. The lipids produced under these conditions were rich in saturated fatty acids (FAs) and monounsaturated FAs, with no polyunsaturated FAs in both microalgae. Moreover, several biodiesel characteristics were estimated, and results fell within the ranges specified by international standards. These findings indicate that the mixotrophic cultivation of alkaliphilic microalgae could open new avenues for promoting microalgae productivity through low-cost biofuel production.

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Section: Discussionsupporting

confidence: 91%

Enhancement of biomass productivity and biochemical composition of alkaliphilic microalgae by mixotrophic cultivation using cheese whey for biofuel production

Youssef,

Gomaa,

Mohamed

et al. 2024

Environ Sci Pollut Res

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“…The most important species for significant biomass production are those with faster growth rates and greater biomass productivity [21,37]. These characteristics include selective advantages such as lower contamination from most competitive organisms, a high biomass yield and a substantial reduction in production expenses during large-scale cultivation [38].…”

Section: Biomass and Biomass Productivities Under Different Nutrient Concentrationsmentioning

confidence: 99%

Influence of Nutrient Manipulation on Growth and Biochemical Constituent in Anabaena variabilis and Nostoc muscorum to Enhance Biodiesel Production

et al. 2021

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The present study aims to improve biomass and biochemical constituents, especially lipid production of Anabaena variabilis and Nostoc muscorum by formulating an optimal growth condition using various concentrations of nutrients (NO3−, PO43− and CO32−) for biodiesel production. The supplementation of the three nutrients by +50% showed the maximum dry weight and biomass productivity, while the macromolecule contents were varied. The depletion of N-NO3− by 50% N-NO3− showed the maximum lipid yield (146.67 mg L−1) in A. variabilis and the maximum carbohydrate contents (285.33 mg L−1) in N. muscorum with an increase of 35% and 30% over control of the synthetic medium, respectively. However, variation in P-PO43− and C-CO32− showed insignificant improving results for all biochemical compositions in both cyanobacteria. A. variabilis was the superior species for lipid and protein accumulation; however, N. muscorum showed the maximum carbohydrate content. Accordingly, A. variabilis was selected for biodiesel production. In A. variabilis, −50% N-NO3− resulted in 35% higher lipid productivity compared to the control. Furthermore, the fatty acid profile and biodiesel quality-related parameters have improved under this condition. This study has revealed the strategies to improve A. variabilis lipid productivity for biodiesel production for small-scale in vitro application in terms of fuel quality under low nitrate levels.

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“…It is known that cyanobacterial lipid content could be enhanced between 7 and 32% of the biomass composition by manipulating culture conditions like temperature, light, salinity, carbon dioxide concentration, nitrogen starvation, phosphorus deficiency, and exogenous stresses such as ultrasonication, ultraviolet radiation, and TiO2 [ 25 , 26 ]. In a study by Singh et al [ 27 ] it was reported that although Leptolyngbya foveolarum growth was proficient at pH ranging from 6.5 to 9.0, the maximal lipid content was achieved at pH 7.5. As Nile red and BODIPY have different characteristics in their hydrophilic domains and fluorescent emissions, Nile red is preferred for the detection of triacylglycerols (TAGs), but BODIPY for lipid droplets [ 15 ].…”

Section: Resultsmentioning

confidence: 99%

Lipid production and cellular changes in Fremyella diplosiphon exposed to nanoscale zerovalent iron nanoparticles and ampicillin

et al. 2023

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With the dramatic decrease in fossil fuel stocks and their detrimental effects on the environment, renewable energy sources have gained imminent importance in the mitigation of emissions. As lipid-enriched energy stocks, cyanobacteria are the leading group of microorganisms contributing to the advent of a new energy era. In the present study, the impact of Nanofer 25 s nanoscale zero-valent iron nanoparticles (nZVIs) and ampicillin on lipid production and cellular structural changes in Fremyella diplosiphon strain B481-SD were investigated. Total lipid abundance, fatty acid methyl ester (FAME) compositions, and alkene production as detected by high-resolution two-dimensional gas chromatography with time-of-flight mass spectrometry (GC × GC/TOF–MS) was significantly higher (p < 0.05) in the individual application of 0.8 mg/L ampicillin, 3.2 mg/L nZVIs, and a combined regimen of 0.8 mg/L ampicillin and 3.2 mg/L nZVIs compared to the untreated control. In addition, we identified significant increases (p < 0.05) in monounsaturated fatty acids (MUFAs) in F. diplosiphon treated with the combination regimen compared to the untreated control, 0.8 mg/L of ampicillin, and 3.2 mg/L of nZVIs. Furthermore, individual treatment with 0.8 mg/L ampicillin and the combination regimen (0.8 mg/L ampicillin + 3.2 mg/L nZVIs) significantly increased (p < 0.05) Nile red fluorescence compared to the untreated control, indicating neutral membrane lipids to be the main target of ampicillin added treatments. Transmission electron microscopy studies revealed the presence of single-layered thylakoid membranes in the untreated control, while complex stacked membranes of 5–8 layers were visualized in ampicillin and nZVI-treated F. diplosiphon. Our results indicate that nZVIs in combination with ampicillin significantly enhanced total lipids, essential FAMEs, and alkenes in F. diplosiphon. These findings offer a promising approach to augment the potential of using the strain as a large-scale biofuel agent.

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Biomass and Lipid Productivities of Cyanobacteria- Leptolyngbya foveolarum HNBGU001

Cited by 23 publications

References 46 publications

Enhancement of biomass productivity and biochemical composition of alkaliphilic microalgae by mixotrophic cultivation using cheese whey for biofuel production

Enhancement of biomass productivity and biochemical composition of alkaliphilic microalgae by mixotrophic cultivation using cheese whey for biofuel production

Influence of Nutrient Manipulation on Growth and Biochemical Constituent in Anabaena variabilis and Nostoc muscorum to Enhance Biodiesel Production

Lipid production and cellular changes in Fremyella diplosiphon exposed to nanoscale zerovalent iron nanoparticles and ampicillin

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