Biomass and RRR-α-tocopherol production in Stichococcus bacillaris strain siva2011 in a balloon bioreactor

Abstract

BackgroundGreen microalgae represent a renewable natural source of vitamin E. Its most bioactive form is the naturally occurring RRR-α-tocopherol which is biosynthesized in photosynthetic organisms as a single stereoisomer. It is noteworthy that the natural and synthetic α-tocopherols are different biomolecular entities. This article focuses on RRR-α-tocopherol production in Stichococcus bacillaris strain siva2011 biomass in a bioreactor culture with methyl jasmonate (MeJa) elicitor. Additionally, a nonlinear mathematical model was used to quantitatively scale-up and predict the biomass production in a 20 L balloon bioreactor with dual variables such as time and volume.ResultsApproximately 0.6 mg/g dry weight (DW) of RRR-α-tocopherol was enhanced in S. bacillaris strain siva2011 biomass with the MeJa 50 μL/L for 24 hrs elicitations when compared to the control. The R2 value from the nonlinear model was enhanced up to 95% when compared to the linear model which significantly improved the accuracy for estimating S. bacillaris strain siva2011 biomass production in a balloon bioreactor.ConclusionsS. bacillaris strain siva2011 is a new green microalga which biosynthesizes significant amounts of RRR-α-tocopherol. Systematically validated dual variable empirical data should provide key insights to multivariable or fourth order modeling for algal biomass scale-up. This bioprocess engineering should provide valuable information for industrial production of RRR-α-tocopherol from green cells.

Enigmatic Microalgae from Aeroterrestrial and Extreme Habitats in Cosmetics: The Potential of the Untapped Natural Sources

“…Among the tested algae, 37 were aeroterrestrial and some of them contained high amounts (up to 1062.02 µg g −1 dm) of α-tocopherol: the green Bracteacoccus sp., Coccomyxa sp., Muriella terrestris, Stichococcus bacillaris, yellow-green Botrydiopsis intercedens, Heterococcus sp., Xanthonema sp., eustigmatophycean Monodus guttula, etc. Currently, the most bioactive form of vitamin E-the naturally occurring RRR-α-tocopherol-was discovered in Stichococcus bacillaris [76]. Considering the worldwide distribution of this aeroterrestrial filamentous alga [53], we note it as important potential renewable natural source of vitamin E. Two cryophilic species of green algae-Chlamydocapsa sp.…”

Application of Marine Microbial Natural Products in Cosmetics

“…Among them, the α-tocopherol production of Chlorophyta was higher than that of Rhodophyta, which could be a natural source of α-tocopherol ( Mudimu et al, 2017 ). Sivakumar et al (2014) discovered a new strain ( Stichococcus bacillaris strain siva2011) that produces natural RRR-α-tocopherol and could be commercialized. Leya et al (2009) found two strains of Raphidonema from snow and permafrost substrates, which were good α-tocopherol producers ( Leya et al, 2009 ).…”

The progress of the biosynthesis of vitamin E

“…植物中生育酚合成的关键前体PDP主要来源 于叶绿素的降解和循环利用 [13,19] , 但参与叶绿素降解 . 在裂丝藻中添加茉莉酸甲 酯作为诱导剂来调节与藻类应激胁迫相关的基因表达 也可以增加特定异构体的生育酚的产量 [25] . 但藻类特 殊的培养条件限制了它的工业应用.…”