Biomass derived self-assembled DNA-dot hydrogels for enhanced bacterial annihilation

Abstract

Nanotization of biomass for interesting biomedical applications is still in nascent stage with no visible market available products. While products derived from biomass DNA and protein have unquestionable biocompatibility, induction...

A biomass-derived dual crosslinked DNA-nanoparticle hydrogel for visible light-induced photodynamic bacterial inactivation

“…The calculated fluorescence quantum yield (QY) of the optimized DNA dot was ∼5.33% with respect to the standard quinine sulphate (QY = 0.55) in 0.5 M H 2 SO 4 which happens to be the highest among other DNA-based nanoparticles reported so far. 15…”

“…This shows the importance of adding untransformed genomic DNA as a filler or a matrix material to obtain the hydrogel. 15,16…”

“…At a predetermined time, the solution from the reaction medium was removed for spectroscopic analysis. 15,26 Following ROS generation from the DNA-NP, the non-fluorescent DHR123 (2 mL, 2.5 nM) was oxidised to green fluorescent rhodamine 123. At definite time intervals, fluorescence spectra in the wavelength region of 490-600 nm were captured (480 nm excitation wavelength).…”

“…The major advantage of using DNA-NP as a material for hydrogels is the possibility of further crosslinking through hybridization-mediated self-assembly. 15,16 Such self-assembled crosslinking could be achieved through simple thermal annealing. The resulting hydrogel becomes temperature sensitive since the hydrogen bonds created during the self-assembly are heat labile.…”

Intelligent DNA-Based Hydrogels for Bioanalysis and Therapeutics

“…Therefore, biomass DNA conversion is more attractive than other biomolecules. In 2022, Das’s group reported for the first time that the biomass DNA derived from onion is transformed into DNA point by partial hydrothermal pyrolysis to improve its mechanical and photophysical properties; then hydrogels were created by hybridization-mediated self-assembly with untransformed genomic DNA using DNA dots as cross-linking agents. The preparation method of DNA hydrogel is that the DNA site and the untransformed biomass genomic DNA are annealed at 95 °C for 10 min and then slowly cooled so that the genomic DNA of the DNA site and the surface DNA fragment are hybridized and linked.…”