Biomass measurement of living Lumbriculus variegatus with impedance spectroscopy

Abstract

Impedance spectroscopy is a useful tool for non-invasive and real time measurements of cell suspensions and a variety of biological tissues. The objective of this study was the investigation of the dielectric properties of living aquatic worms (Lumbriculus variegatus) using impedance spectroscopy in a frequency range between 100 Hz and 10 MHz. We demonstrate a linear relation between the worm biomass and the phase response of the signal thereby providing a quick and precise method to determine the biomass of aquatic worms in situ. Possible applications for nondestructive online biomass monitoring of aquatic worms and other aqueous organisms are discussed. Furthermore, we show that groups of worms fed different diets can be distinguished by the method presented. These results reveal a close relationship between the nutritional composition of the worms and the measured phase response. We also demonstrate that the phase response at 90 kHz does not depend on the worm size. In contrast, the response function for the signal at 440 Hz reveals a linear correlation of average individual worm size and phase. Therefore, we conclude that the measured phase response at 90 kHz qualifies as a measure of the total amount of worm biomass present in the measuring cell, whereas the phase measurement at 440 Hz can be used to estimate the average individual worm size.

Strong Gradients in Weak Magnetic Fields Induce DOLLOP Formation in Tap Water

“…Electrode polarisation has no direct electric circuit equivalent, but can be simulated as a combination of certain elements [33]: A constant phase element (CPE) [34] with a Warburg impedance (W) in parallel to account for ion migration; R and W impedance represent bulk properties of the electrolyte solution and diffusion features of the probe in the solution [35]. The formation of DOLLOPs should thus be detectable by EIS in a threefold manner: the increase of R aq due to the lower number of ions available, the decrease of the electrode polarisation for the same reason, and the inability of the (much heavier) DOLLOPs to follow the electric field and build layers, which should appear as a change of the CPE and W parameters, respectively.…”

Design and development of microcontroller based instrumentation for studying complex bioelectrical impedance of fruits using electrical impedance spectroscopy

“…Due to its significant capability of electrical impedance based material characterization, EIS has been applied in biomedical engineering (Bera, ; Bera & Nagaraju, ; Bera, Nagaraju, & Lubineau, ; Bera et al, ; Birgersson, Birgersson, & Ollmar, ; Chakraborty et al, ; Keshtkar, ; Röthlingshöfer, Ulbrich, Hahne, & Leonhardt, ; Ruiz, Zamora, & Felice, ; Sammer et al, ), material engineering (Grassini, Corbellini, Parvis, Angelini, & Zucchi, in press; Grossi, Lanzoni, Lazzarini, & Riccò, ; Greuter & Blatter, ; YanLong, Bera, Lubineau, & Yang ; ; He & Mansfeld, ; Morrison, Sinclair, & West, ; Pulido et al, ), chemical engineering (Adachi, Sakamoto, Jiu, Ogata, & Isoda, ; Echabaane, Rouis, Bonnamour, & Ouada, ; Kern, Sastrawan, Ferber, Stangl, & Luther, ; Longo, Nogueira, De Paoli, & Cachet, ; Lee, Hwang, Mashek, J. J., & Mason, ; Song, Jung, Lee, & Dao, ; Wang, Moser, & Grätzel, ), civil engineering (Christensen et al, ; Ribeiro et al, ), and other fields of applied sciences and engineering (Almuhammadi et al, ; Bera et al, ). EIS is found as a widely used method suitable for investigating biological tissues non‐invasively (Arpaia, Clemente, & Romanucci, ; Bera, ; Bera & Nagaraju, ; Bera & Nagaraju, ; Bera et al, , ; Birgersson et al, ; Clemente, Arpaia, & Manna, ; Clemente, Romano, Bifulco, & Cesarelli, ; Keshtkar, ; Röthlingshöfer et al, ; Ruiz et al, ; Sammer et al, ). Impedance spectroscopy can also be used for food materials such as meat (Bai et al, ; Oliver et al, ; Zhao et al, ), fish (Niu & Lee, ; Oliver et al, ), bread (Bhatt & Nagaraju, ; Daikuzono et al, ) and fruits and vegetables (Bera & Nagaraju, ; Bera, Bera, Chowdhury, Ghoshal, & Chakraborty, ...…”

“…biomedical engineering (Bera, 2014;Bera & Nagaraju, 2011b;Bera, Nagaraju, & Lubineau, 2016b;Bera et al, 2013;Birgersson, Birgersson, & Ollmar, 2012;Chakraborty et al, 2015;Keshtkar, 2007;R€ othlingsh€ ofer, Ulbrich, Hahne, & Leonhardt, 2011;Ruiz, Zamora, & Felice, 2014;Sammer et al, 2014), material engineering (Grassini, Corbellini, Parvis, Angelini, & Zucchi, in press;Grossi, Lanzoni, Lazzarini, & Ricc o, 2012;Greuter & Blatter, 1990;YanLong, Bera, Lubineau, & Yang 2017a;He & Mansfeld, 2009;Morrison, Sinclair, & West, 2001;Pulido et al, 2017), chemical engineering (Adachi, Sakamoto, Jiu, Ogata, & Isoda, 2006;Echabaane, Rouis, Bonnamour, & Ouada, 2013;Kern, Sastrawan, Ferber, Stangl, & Luther, 2002;Longo, Nogueira, De Paoli, & Cachet, 2002;Lee, Hwang, Mashek, J. J., & Mason, 1995;Song, Jung, Lee, & Dao, 1999;Wang, Moser, & Grätzel, 2005), civil engineering (Christensen et al, 1994;Ribeiro et al, 2011), and other fields of applied sciences and engineering (Almuhammadi et al, 2017;Bera et al, 2014).…”

“…EIS is found as a widely used method suitable for investigating biological tissues non-invasively (Arpaia, Clemente, & Romanucci, 2008;Bera, 2014;Bera & Nagaraju, 2011b;Bera & Nagaraju, 2013;Bera et al, 2016aBera et al, , 2016bBirgersson et al, 2012;Clemente, Arpaia, & Manna, 2013;Clemente, Romano, Bifulco, & Cesarelli, 2014;Keshtkar, 2007;R€ othlingsh€ ofer et al, 2011;Ruiz et al, 2014;Sammer et al, 2014).…”

A LabVIEW-based electrical bioimpedance spectroscopic data interpreter (LEBISDI) for biological tissue impedance analysis and equivalent circuit modelling

“…Because bioelectrical impedance depends on the physiological and physiochemical status of the probed tissue and because it also varies from subject to subject and with changes in the health status of the tissue [5][6], such as inflammation, infection and disease, studying how a tissue responds to frequency can provide valuable information about its anatomy and physiology. Many researchers [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] have investigated electrical bioimpedance as an effective, noninvasive method to probe the pathological status of biological tissues.…”