Biomass of Sediment Bacteria by Sedimentation Field-Flow Fractionation

SdFFF monitoring of cellular apoptosis induction by diosgenin and different inducers in the human 1547 osteosarcoma cell line

“…As described [4,5,15,16], by taking advantage of intrinsic cell biophysical properties: size, density, rigidity or shape; SdFFF sorts viable cells which can be cultured for further use without specific labeling of any kind, in few minutes. Since the pioneering report of Caldwell et al [6], which defined most of the basic rules and methodologies for cell separation, FFF, SdFFF and related technologies have shown a great potential for cell separation and purification with major biomedical applications including hematology [11,17,18], cancer research [18][19][20], microorganism analysis [21][22][23][24][25][26][27], biochemistry and molecular biology [28][29][30]. More recently, we opened the field of neuroscience with the purification of neurons from a complex cell matrix [15], and provided sterile, usable and purified immature neural cell fractions without induction of cell differentiation [16].…”

Micro-thermal field-flow fractionation of bacteria

“…the application of SFFF which, in the meantime, became the most frequently used FFF technique when taking into account the number of published papers [1][2][3][4][5][6][7][8][9][10][11]. However, it seems that the use of FFFF, firstly applied in 1996 by Ross et al [3], is more intensively studied in the last 10 years [1,3,7,10,[12][13][14][15][16].…”

“…All three mentioned FFF methods were used also for the separation and analysis of various bacteria [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16]. The first paper, published already in 1985 by Fox et al [1], concerned * Corresponding author.…”

Neural stem cell separation from the embryonic avian olfactory epithelium by sedimentation field-flow fractionation

“…Thus, SdFFF device setup and elution conditions should be optimized to promote the "Hyperlayer" elution mode enhancing a better sub-population separation in association with a drastic limitation of cell-wall interactions [3]. Since the pioneering report of Caldwell et al [2], FFF, SdFFF and related technologies have shown a great potential for cell separation and purification with major biomedical applications including hematology, cancer research or molecular biology [14][15][16][17][18][19][20][21][22]. In a recent paper [5], we have shown the interest in ES cell (mouse embryonic stem cells) sorting to select the most appropriate population to obtain transgenic mice.…”