Biomass retention and microbial segregation to offset the impacts of seasonal temperatures for a pilot-scale integrated fixed-film activated sludge partial nitritation-anammox (IFAS-PN/A) treating anaerobically pretreated municipal wastewater

Rong, Chao; Luo, Zibin; Wang, Tianjie; Qin, Yu; Wu, Jiang; Guo, Yan; Hu, Yisong; Kong, Zhe; Hanaoka, Taira; Sakemi, Shinichi; Ito, Masami; Kobayashi, Shigeki; Kobayashi, Masumi; Li, Yu-You

doi:10.1016/j.watres.2022.119194

Cited by 48 publications

(14 citation statements)

References 43 publications

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“…In contrast, the PD process had a significant advantage in the stable efficient nitrite production for anammox, as demonstrated in this study. Additionally, ammonia-oxidizing bacteria and anammox bacteria inhibition have been observed at a low temperature of about 15 °C in a PN/A granular system . Although high nitrogen removal performance has been reported in previous PD/A studies, the much higher NLR in this study demonstrates a significant advantage of sludge granulation.…”

Section: Resultscontrasting

confidence: 45%

“…Additionally, ammonia-oxidizing bacteria and anammox bacteria inhibition have been observed at a low temperature of about 15 °C in a PN/A granular system. 28 Although high nitrogen removal performance has been reported in previous PD/A studies, the much higher NLR in this study demonstrates a significant advantage of sludge granulation. The efficient retention of slow-growth bacteria and balanced activities between denitrifiers and anammox bacteria were crucial for the stable performance of the PD/A system operating at seasonal temperature.…”

Section: Microbial Community Analysiscontrasting

confidence: 51%

“…Although the PD process provides an efficient solution, the stable coexistence of denitrifying bacteria and anammox bacteria is important but still uncertain . The relatively slow growth rate of anammox bacteria may lead to low activity and reduced competitiveness with denitrifying bacteria, , and the washout of anammox bacteria may occur, further worsening the stability …”

Section: Resultsmentioning

confidence: 99%

“…6 Although the PD process provides an efficient solution, the stable coexistence of denitrifying bacteria and anammox bacteria is important but still uncertain. 48 The relatively slow growth rate of anammox bacteria may lead to low activity and reduced competitiveness with denitrifying bacteria, 28,34 and the washout of anammox bacteria may occur, further worsening the stability. 49 In this study, it was observed that the PD/A granular system was able to maintain a stable and efficient performance at temperature below 10 °C, highlighting the stable cooperation between denitrifying bacteria and anammox.…”

Section: Spatial Distribution Of Functional Bacteriamentioning

confidence: 99%

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Spatiotemporal Assembly and Immigration of Heterotrophic and Anammox Bacteria Allow a Robust Synergy for High-Rate Nitrogen Removal

Du,

Liu,

et al. 2023

Environ. Sci. Technol.

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The novel partial denitrification-driven anammox (PD/A) is an energy-efficient method for nitrogen removal from wastewater. However, its stability and efficiency are impeded by the competition between heterotrophic denitrifying bacteria and relatively slow-growing anammox bacteria. In this study, a PD/A granular sludge system was developed, which achieved a nitrogen removal efficiency of 94% with 98% anammox contribution, even as the temperature dropped to 9.6 °C. Analysis of bacterial activity in aggregates of different sizes revealed that the largest granules (>2.0 mm) exhibited the highest anammox activity, 2.8 times that of flocs (<0.2 mm), while the flocs showed significantly higher nitrite production rates of PD, more than six times that of the largest granules. Interestingly, fluorescent in situ hybridization (FISH) combined with confocal laser scanning microscopy (CLSM) revealed a nest-shaped structure of PD/A granules. The Thauera genus, a key contributor to PD, was highly enriched at the outer edge, providing substrate nitrite for anammox bacteria inside the granules. As temperature decreased, the flocs transformed into small granules to efficiently retain anammox bacteria. This study provides multidimensional insights into the spatiotemporal assembly and immigration of heterotrophic and autotrophic bacteria for stable and high-rate nitrogen removal.

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Section: Resultscontrasting

confidence: 45%

Section: Microbial Community Analysiscontrasting

confidence: 51%

Section: Resultsmentioning

confidence: 99%

Section: Spatial Distribution Of Functional Bacteriamentioning

confidence: 99%

See 2 more Smart Citations

Spatiotemporal Assembly and Immigration of Heterotrophic and Anammox Bacteria Allow a Robust Synergy for High-Rate Nitrogen Removal

Du,

Liu,

et al. 2023

Environ. Sci. Technol.

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“…The low temperature of domestic wastewater is a major challenge for mainstream PN/A. − Here, a high nitrogen removal efficiency (88.9 ± 2.5%) was stably achieved in treating domestic wastewater at temperatures as low as 12 °C. The nitrogen loading rate of the developed two-stage PN/A was ∼0.1 kg N/m 3 /days.…”

Section: Resultsmentioning

confidence: 97%

Toward Energy Neutrality: Novel Wastewater Treatment Incorporating Acidophilic Ammonia Oxidation

Liu

Wang

et al. 2023

Environ. Sci. Technol.

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Chemically enhanced primary treatment (CEPT) followed by partial nitritation and anammox (PN/A) and anaerobic digestion (AD) is a promising roadmap to achieve energy-neutral wastewater treatment. However, the acidification of wastewater caused by ferric hydrolysis in CEPT and how to achieve stable suppression of nitrite-oxidizing bacteria (NOB) in PN/A challenge this paradigm in practice. This study proposes a novel wastewater treatment scheme to overcome these challenges. Results showed that, by dosing FeCl 3 at 50 mg Fe/L, the CEPT process removed 61.8% of COD and 90.1% of phosphate and reduced the alkalinity as well. Feeding by low alkalinity wastewater, stable nitrite accumulation was achieved in an aerobic reactor operated at pH 4.35 aided by a novel acid-tolerant ammonium-oxidizing bacteria (AOB), namely, Candidatus Nitrosoglobus. After polishing in a following anoxic reactor (anammox), a satisfactory effluent, containing COD at 41.9 ± 11.2 mg/L, total nitrogen at 5.1 ± 1.8 mg N/ L, and phosphate at 0.3 ± 0.2 mg P/L, was achieved. Moreover, the stable performances of this integration were well maintained at an operating temperature of 12 °C, and 10 investigated micropollutants were removed from the wastewater. An energy balance assessment indicated that the integrated system could achieve energy self-sufficiency in domestic wastewater treatment.

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Biochar‐Assisted Anaerobic Ammonium Oxidation

Tian,

Tang,

et al. 2023

Biochar Applications for Wastewater Treatment

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Biomass retention and microbial segregation to offset the impacts of seasonal temperatures for a pilot-scale integrated fixed-film activated sludge partial nitritation-anammox (IFAS-PN/A) treating anaerobically pretreated municipal wastewater

Cited by 48 publications

References 43 publications

Spatiotemporal Assembly and Immigration of Heterotrophic and Anammox Bacteria Allow a Robust Synergy for High-Rate Nitrogen Removal

Spatiotemporal Assembly and Immigration of Heterotrophic and Anammox Bacteria Allow a Robust Synergy for High-Rate Nitrogen Removal

Toward Energy Neutrality: Novel Wastewater Treatment Incorporating Acidophilic Ammonia Oxidation

Biochar‐Assisted Anaerobic Ammonium Oxidation

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