“…Depending on substrate availability and mass transfer processes, biomat growth will cause increased resistance to water flow (Rosenzweig et al, 2014) and, thus, contribute to the eventual distribution of effluent along the entire length of STU trenches (Beach et al, 2005;McKinley and Siegrist, 2011;Siegrist and Boyle, 1987;Winstanley and Fowler, 2013). As soil microorganisms are regularly challenged by changing moisture conditions potentially reducing substrate diffusion, restricting motility and experiencing desiccation stress in the soil environment, a mature biomat may serve as a protective barrier to ensure efficient soil treatment and variably saturated condition in the underlying soil (Geza et al, 2013;McKinley and Siegrist, 2011;Potts, 1994;Roberson and Firestone, 1992).…”