Biomaterial Approaches for Stem Cell-Based Myocardial Tissue Engineering

Cutts, Josh; Nikkhah, Mehdi; Brafman, David A.

doi:10.4137/bmi.s20313

Cited by 29 publications

(35 citation statements)

References 226 publications

(254 reference statements)

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“…The cells have been utilized as therapeutics in both human and animal with encouraging results for the repair of cartilage, tendon, bone, neurodegenerative disorders, spinal injuries, cardiac defects, facilitate wound healing, etc. (Caplan, 2005;Ribitsch et al, 2010;Xie et al, 2012;Gugjoo et al, 2015;Cutts et al, 2015). However, such a potential is yet to be harnessed due to the lack of proper understanding of the cell-tissue interactions, their differentiation and the immune reactions that cells may encounter in host environment.…”

Section: Discussionmentioning

confidence: 99%

“…Adv., 10 (10): 537-548, 2015 (a) (b) etc. (Caplan, 2005;Ribitsch et al, 2010;Xie et al, 2012;Gugjoo et al, 2015;Cutts et al, 2015). However, MSCs obtained from bone marrow constitute a very small fraction, 0.01-0.001% of the bone marrow cells (Pittenger et al, 1999;Martin et al, 2002;Gugjoo et al, 2015) and many cells obtained during harvesting process may be progenitor cells that mimic the characteristic of MSCs (Chong et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…The cells also modulate immune function extending their potential use in allogenic or xenogenic therapies. Due to these characteristic features, MSCs are currently being increasingly used in tissue engineering and regenerative medicine (Tay et al, 2012;Cutts et al, 2015). These cells have been utilized as therapeutics in both human and animal, for the repair of cartilage, tendon, bone treatment of neurodegenerative disorders, spinal injuries, cardiac defects, facilitate wound healing, Asian J.…”

Section: Introductionmentioning

confidence: 99%

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Isolation, Culture and Characterization of New Zealand White Rabbit Mesenchymal Stem Cells Derived from Bone Marrow

Gugjoo¹,

Amarpal²,

Kinjavdeka³

et al. 2015

Asian J. of Animal and Veterinary Advances

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Mesenchymal stem cells are recognised based upon the plastic adherence, fibroblastic morphology, expression of certain surface markers, non-expression of haematopoetic markers and their ability to differentiate into atleast three lineages viz., adipogenic, chondrogenic and osteogenic. The rabbit Mesenchymal Stem Cells (rMSCs) though used extensively in research but have not been thoroughly studied and are not compared to other species. The present study was therefore conducted to determine the morphology, surface markers and trilineage differentiation potential of New Zealand white rabbit MSCs. Isolation of rMSCs was done by an established method of density gradient method using Ficoll-hapaque. The cells were characterised by Phase Contrast Microscopy, Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Alkaline Phosphatase (AP) staining. The cells isolated were plastic adherent and had fibroblastic spindle shape with eccentric irregular nuclei. The cells expressed surface markers viz., CD 105 and CD 106 besides expressing genes of collagen type II and I. Haematopoetic markers (CD 34 and CD 45) and aggrecan gene however, were not expressed. The rMSCs showed a moderate alkaline phosphates activity. Trilineage differentiation was conducted utilizing prepared differentiation media and rMSCs were differentiated into corresponding cell lineages based upon the medium used. It was concluded that rMSCs possess morphology similar to other species with good proliferation rate and exhibit the characteristics laid down by International Society for Cellular Therapy (ISCT). The present study provided basic protocols for characterization of rabbit MSCs that should be used before application of these cells for any research or therapy.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation, Culture and Characterization of New Zealand White Rabbit Mesenchymal Stem Cells Derived from Bone Marrow

Gugjoo¹,

Amarpal²,

Kinjavdeka³

et al. 2015

Asian J. of Animal and Veterinary Advances

View full text Add to dashboard Cite

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“…These are: cellular preconditioning, prevention of senescence, and rejuvenation of stem cells. We noticed there are additional strategies to improve the effectiveness of stem cell therapy, such as stem cell conjugation with biomaterials [6], boosting the endogenous cardiac regeneration [7, 8], etc., all of which have been recently well-described in many other places [6–8], and will not be discussed here due to the page limitation. Each of these strategies is summarized in Fig.…”

Section: Introductionmentioning

confidence: 99%

Strategies to Enhance the Effectiveness of Adult Stem Cell Therapy for Ischemic Heart Diseases Affecting the Elderly Patients

Khatiwala

Cai

2016

Stem Cell Rev and Rep

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Myocardial infarctions and chronic ischemic heart disease both commonly and disproportionately affect elderly patients more than any other patient population. Despite available treatments, heart tissue is often permanently damaged as a result of cardiac injury. This review aims to summarize recent literature proposing the use of modified autologous adult stem cells to promote healing of post-infarct cardiac tissue. This novel cellular treatment involves isolation of adult stem cells from the patient, in vitro manipulation of these stem cells, and subsequent transplantation back into the patient’s own heart to accelerate healing. One of the hindrances affecting this process is that cardiac issues are increasingly common in elderly patients, and stem cells recovered from their tissues tend to be pre-senescent or already in senescence. As a result, harsh in vitro manipulations can cause the aged stem cells to undergo massive in vivo apoptosis after transplantation. The consensus in literature is that inhibition or reversal of senescence onset in adult stem cells would be of utmost benefit. In fact, it is believed that this strategy may lower stem cell mortality and coerce aged stem cells into adopting more resilient phenotypes similar to that of their younger counterparts. This review will discuss a selection of the most efficient and most-recent strategies used experimentally to enhance the effectiveness of current stem cell therapies for ischemic heart diseases.

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“…Recently, with the emergence of myocardial tissue engineering, the delivery of ex vivo bone mesenchymal stem cells (MSCs) to the infarcted heart has been successfully performed (1)(2)(3).…”

Section: Introductionmentioning

confidence: 99%

TGF‑β1 combined with Sal‑B promotes cardiomyocyte differentiation of rat mesenchymal stem cells

Liu

et al. 2018

Exp Ther Med

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Transforming growth factor β1 (TGF-β1) and salvianolic acid B (Sal-B) are key signaling factors for stem cell differentiation into cardiomyocytes (CMs). The present study compared the biological effect of TGF-β1 and Sal-B, alone or in combination, on bone marrow mesenchymal stromal cells (BMSCs) that differentiate into myocardial-like cells in a simulated myocardial microenvironment . BMSCs were isolated from bones of limbs of 10 male Sprague Dawley rats and cultured. The 2nd-generation BMSCs were co-incubated with TGF-β1 and Sal-B, alone or in combination, for 72 h. The control group was BMSCs cultured without any inductive substance. The levels GATA binding protein 4 (GATA4) and homeobox protein NKx2.5 were determined by reverse-transcription quantitative polymerase chain reaction and immunofluorescence staining was used to evaluate α-sarcomeric actin and cardiac troponin I (cTNI) as cardiomyogenic differentiation markers. The ultrastructure of BMSCs in each group was also observed. BMSCs were initially spindle-shaped with irregular processes. The cells gradually increased in number 24 h post-inoculation and proliferated 7 days later. Compared with the control group, BMSCs in the treatment groups had fusiform shapes, orientating with one accord and were connected with adjoining cells forming myotube-like structures on day 28. The morphology and architecture/myotubes of BMSCs was similar among the treatment groups, but the amount of cells in the combined group was comparatively higher. The results of immunofluorescence staining revealed the expression of the CM-specific proteins α-sarcomeric actin and cTNI in these cells. The expression of these cardiac-specific markers in the combined group was significantly higher than that in the other groups (P<0.01 or P<0.05). In addition, the transcriptional expression of GATA4 and NKx2.5 in the treatment groups was stable and significantly higher than that in the control group on day 7. Transmission electron microscopy showed that BMSCs in the treatment groups all had myofilaments, rough endoplasmic reticulum and mitochondria in the cytoplasm when compared with the control group. Taken together, these results indicated that the combination of TGF-β1 and Sal-B effectively promotes cardiomyogenic differentiation of BMSCs and their application may represent a therapeutic strategy for the treatment of ischemic heart disease.

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Biomaterial Approaches for Stem Cell-Based Myocardial Tissue Engineering

Cited by 29 publications

References 226 publications

Isolation, Culture and Characterization of New Zealand White Rabbit Mesenchymal Stem Cells Derived from Bone Marrow

Isolation, Culture and Characterization of New Zealand White Rabbit Mesenchymal Stem Cells Derived from Bone Marrow

Strategies to Enhance the Effectiveness of Adult Stem Cell Therapy for Ischemic Heart Diseases Affecting the Elderly Patients

TGF‑β1 combined with Sal‑B promotes cardiomyocyte differentiation of rat mesenchymal stem cells

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