Biomaterial-Assisted Regenerative Medicine

Nii, Takenobu; Katayama, Yoshiki

doi:10.3390/ijms22168657

Cited by 65 publications

(57 citation statements)

References 169 publications

(193 reference statements)

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“…Three-dimensional (3D) tissue culture techniques containing various functional substrates (collagen, fibronectin, laminin, and gelatin), and multi-cellular components which recreate the tumor microenvironment more accurately have been developed [ 189 , 190 ]. These new in vitro techniques are valuable tools for studying tumor biology and would allow a better understanding of cellular interactions within the tumor microenvironment that could be possibly used for targeted therapy development [ 191 , 192 ].…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

The Breast Tumor Microenvironment: A Key Player in Metastatic Spread

Terceiro

Edechi

Ikeogu

et al. 2021

Cancers

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The tumor microenvironment plays a pivotal role in the tumorigenesis, progression, and metastatic spread of many cancers including breast. There is now increasing evidence to support the observations that a bidirectional interplay between breast cancer cells and stromal cells exists within the tumor and the tumor microenvironment both at the primary tumor site and at the metastatic site. This interaction occurs through direct cell to cell contact, or by the release of autocrine or paracrine factors which can activate pro-tumor signaling pathways and modulate tumor behavior. In this review, we will highlight recent advances in our current knowledge about the multiple interactions between breast cancer cells and neighboring cells (fibroblasts, endothelial cells, adipocytes, innate and adaptive immune cells) in the tumor microenvironment that coordinate to regulate metastasis. We also highlight the role of exosomes and circulating tumor cells in facilitating breast cancer metastasis. We discuss some key markers associated with stromal cells in the breast tumor environment and their potential to predict patient survival and guide treatment. Finally, we will provide some brief perspectives on how current technologies may lead to the development of more effective therapies for the clinical management of breast cancer patients.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

The Breast Tumor Microenvironment: A Key Player in Metastatic Spread

Terceiro

Edechi

Ikeogu

et al. 2021

Cancers

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“…Not only cancer research but also fields such as regenerative medicine will benefit from the use of different 3D biomaterials to effectively support cell culture, improve cell transplantation, and as platforms for drug research on drug screening [ 173 ].…”

Section: Future Directionsmentioning

confidence: 99%

Mechanical Studies of the Third Dimension in Cancer: From 2D to 3D Model

Paradiso

Serpelloni

Francis

et al. 2021

IJMS

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From the development of self-aggregating, scaffold-free multicellular spheroids to the inclusion of scaffold systems, 3D models have progressively increased in complexity to better mimic native tissues. The inclusion of a third dimension in cancer models allows researchers to zoom out from a significant but limited cancer cell research approach to a wider investigation of the tumor microenvironment. This model can include multiple cell types and many elements from the extracellular matrix (ECM), which provides mechanical support for the tissue, mediates cell-microenvironment interactions, and plays a key role in cancer cell invasion. Both biochemical and biophysical signals from the extracellular space strongly influence cell fate, the epigenetic landscape, and gene expression. Specifically, a detailed mechanistic understanding of tumor cell-ECM interactions, especially during cancer invasion, is lacking. In this review, we focus on the latest achievements in the study of ECM biomechanics and mechanosensing in cancer on 3D scaffold-based and scaffold-free models, focusing on each platform’s level of complexity, up-to-date mechanical tests performed, limitations, and potential for further improvements.

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“…Tissue engineering (TE) has become a major field of research in regenerative medicine [1], including areas such as bone [2], skin [3], muscle [4], cancer [5], and cardiac TE [6]. Suitable biomaterials of natural or synthetic origin have central importance for the success of regenerative TE and must thus fulfill pivotal pre-requisites concerning biocompatibility, biodegradability (generally desired), morphology, pore size/porosity, and mechanical strength [7].…”

Section: Introductionmentioning

confidence: 99%

“…The original choice of PGA as a biomaterial was based on: (i) longterm experience and use as a medical device for, e.g., suture material, osteofixation, and cartilage repair [2,7,25,26]; (ii) its character as a non-natural, synthetic product, avoiding problems such as rejection for religious reasons and virus or prion contamination but allowing easy melting, processing, or spinning for manufacturing [7]; (iii) its complete resorption in vivo within 3 months [27], following hydrolytic breakdown into natural degradation products [7]. In this context, potential induction of inflammation and osteolysis by breakdown products of such synthetic biopolymers may be more of a problem for bulk screw and osteofixation biomaterials than for scaffolds and sutures [1,2,7,25,28]. Additionally, the relative suitability of PGA/PLGA versus alginate, hyaluronic acid, or chitosan, which are similar to and/or interact with local glycosaminoglycans for cartilage regeneration [1], should be assessed by 'contemplating both potential advantages and disadvantages of each technique' [7] and by optimizing the match between 'the final product properties .…”

Section: Introductionmentioning

confidence: 99%

“…In this context, potential induction of inflammation and osteolysis by breakdown products of such synthetic biopolymers may be more of a problem for bulk screw and osteofixation biomaterials than for scaffolds and sutures [1,2,7,25,28]. Additionally, the relative suitability of PGA/PLGA versus alginate, hyaluronic acid, or chitosan, which are similar to and/or interact with local glycosaminoglycans for cartilage regeneration [1], should be assessed by 'contemplating both potential advantages and disadvantages of each technique' [7] and by optimizing the match between 'the final product properties . .…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Cartilage Regeneration with a Three-Dimensional Polyglycolic Acid (PGA) Implant in a Bovine Cartilage Punch Model

Horbert

Xin

Fôhr

et al. 2021

IJMS

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Resorbable polyglycolic acid (PGA) chondrocyte grafts are clinically established for human articular cartilage defects. Long-term implant performance was addressed in a standardized in vitro model. PGA implants (+/− bovine chondrocytes) were placed inside cartilage rings punched out of bovine femoral trochleas (outer Ø 6 mm; inner defect Ø 2 mm) and cultured for 84 days (12 weeks). Cartilage/PGA hybrids were subsequently analyzed by histology (hematoxylin/eosin; safranin O), immunohistochemistry (aggrecan, collagens 1 and 2), protein assays, quantitative real-time polymerase chain reactions, and implant push-out force measurements. Cartilage/PGA hybrids remained vital with intact matrix until 12 weeks, limited loss of proteoglycans from “host” cartilage or cartilage–PGA interface, and progressively diminishing release of proteoglycans into the supernatant. By contrast, the collagen 2 content in cartilage and cartilage–PGA interface remained approximately constant during culture (with only little collagen 1). Both implants (+/− cells) displayed implant colonization and progressively increased aggrecan and collagen 2 mRNA, but significantly decreased push-out forces over time. Cell-loaded PGA showed significantly accelerated cell colonization and significantly extended deposition of aggrecan. Augmented chondrogenic differentiation in PGA and cartilage/PGA-interface for up to 84 days suggests initial cartilage regeneration. Due to the PGA resorbability, however, the model exhibits limitations in assessing the “lateral implant bonding”.

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Biomaterial-Assisted Regenerative Medicine

Cited by 65 publications

References 169 publications

The Breast Tumor Microenvironment: A Key Player in Metastatic Spread

The Breast Tumor Microenvironment: A Key Player in Metastatic Spread

Mechanical Studies of the Third Dimension in Cancer: From 2D to 3D Model

In Vitro Cartilage Regeneration with a Three-Dimensional Polyglycolic Acid (PGA) Implant in a Bovine Cartilage Punch Model

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