Biomaterial granules used for filling bone defects constitute 3D scaffolds: porosity, microarchitecture and molecular composition analyzed by microCT and Raman microspectroscopy

Arbez, Baptiste; Kün‐Darbois, Jean‐Daniel; Convert, Thierry; Guillaume, Bernard; Mercier, Philippe; Hubert, Laurent; Chappard, Daniel

doi:10.1002/jbm.b.34133

Cited by 26 publications

(20 citation statements)

References 41 publications

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“…However, the manufacturer (Kasios SAS) and importer (B.IMTECH, Yongin, Korea) advertised as 99% of β-TCP. Many studies and FDA 510(k) also represented as β-TCP17575859606162636465. It was received human study level III by successful histologic and clinical result comparing Xenograft (BonePlus-xs; Integros, Adana, Turkey) in sinus lift57.…”

Section: Resultsmentioning

confidence: 99%

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Dental alloplastic bone substitutes currently available in Korea

Hong

Lee

et al. 2019

J Korean Assoc Oral Maxillofac Surg

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As dental implant surgery and bone grafts were widely operated in Korean dentist, many bone substitutes are commercially available, currently. For commercially used in Korea, all bone substitutes are firstly evaluated by the Ministry of Health and Welfare (MOHW) for safety and efficacy of the product. After being priced, classified, and registration by the Health Insurance Review and Assessment Service (HIRA), the post-application management is obligatory for the manufacturer (or representative importer) to receive a certificate of Good Manufacturing Practice by Ministry of Food and Drug Safety. Currently, bone substitutes are broadly classified into C group (bone union and fracture fixation), T group (human tissue), L group (general and dental material) and non-insurance material group in MOHW notification No. 2018-248. Among them, bone substitutes classified as dental materials (L7) are divided as xenograft and alloplastic bone graft. The purpose of this paper is to analyze alloplastic bone substitutes of 37 products in MOHW notification No. 2018-248 and to evaluate the reference level based on the ISI Web of Knowledge, PubMed, EMBASE (1980–2019), Cochrane Database, and Google Scholar using the criteria of registered or trademarked product name.

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Section: Resultsmentioning

confidence: 99%

“…It was received human study level III by successful histologic and clinical result comparing Xenograft (BonePlus-xs; Integros, Adana, Turkey) in sinus lift57. Animal, in vivo , and in vitro studies for osteoconductivity5859606162636465.…”

Section: Resultsmentioning

confidence: 99%

Dental alloplastic bone substitutes currently available in Korea

Hong

Lee

et al. 2019

J Korean Assoc Oral Maxillofac Surg

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“…The small size of the animal model necessitated that bone graft particles be morselized prior to their introduction into the tunnel. While some commercial vendors claim that at least some of the efficacy of their bone graft materials is related to particle size [ 60 ], we found that the morselized particles were effective at supporting osteoprogenitor cell attachment and new bone formation ( Figs. 1 , 2 ).…”

Section: Discussionmentioning

confidence: 91%

Bioactivating a bone substitute accelerates graft incorporation in a murine model of vertical ridge augmentation

Chen

Yuan

et al. 2020

Dental Materials

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Objective. Compared to autologous bone grafts, allogeneic bone grafts integrate slowly, which can adversely affect clinical outcomes. Here, our goal was to understand the molecular mechanisms underlying graft incorporation, and then test clinically feasible methods to accelerate this process. Methods. Wild-type and transgenic Wnt “reporter” mice were used in a vertical ridge augmentation procedure. The surgery consisted of tunneling procedure to elevate the maxillary edentulous ridge periosteum, followed by the insertion of bone graft. Micro-computed tomographic imaging, and molecular/cellular analyses were used to follow the bone graft over time. Sclerostin null mice, and mice carrying an activated form of β-catenin were evaluated to understand how elevated Wnt signaling impacted edentulous ridge height and based on these data, a biomimetic strategy was employed to combine bone graft particles with a formulation of recombinant WNT protein. Thereafter, the rate of graft incorporation was evaluated. Results. Tunneling activated osteoprogenitor cell proliferation from the periosteum. If graft particles were present, then osteoprogenitor cells attached to the matrix and gave rise to new bone that augmented edentulous ridge height. Graft particles alone did not stimulate osteoprogenitor cell proliferation. Based on the thicker edentulous ridges in mice with amplified Wnt signaling, a strategy was undertaken to load bone graft particles with WNT; this combination was sufficient to accelerate the initial step of graft incorporation. Significance. Local delivery of a WNT protein therapeutic has the potential to accelerate graft incorporation, and thus shorten the time to when the graft can support a dental implant.

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“…). Numerous studies have been carried out comparing the characteristics of different granular ceramics as bone filling substitutes with those of trabecular bone (Chappard et al ., ; Kerckhofs et al ., ; Arbez et al ., ). When comparing different size of granules, Arbez et al .…”

Section: Xct Characterisation Of Scaffolds and Biomaterialsmentioning

confidence: 97%

“…Numerous studies have been carried out comparing the characteristics of different granular ceramics as bone filling substitutes with those of trabecular bone (Chappard et al, 2015;Kerckhofs et al, 2016;Arbez et al, 2019). When comparing different size of granules, Arbez et al (2019) demonstrated that small granules provided scaffolds with low interconnected pores and may not be favourable for vascular invasion in situ when grafted in a bone defect, whereas the large granules (i.e., 1000-2000 µm) provided 3D scaffolds with interconnectivity well suitable for osteoconduction. XCT also allows for a 3D representation of the pore size within the scaffold (van Lenthe & Müller, 2008;Chappard et al, 2015) and to examine the time-dependent structural characteristics and the in vitro dissolution behaviour of different bioglasess (Westhauser et al, 2017).…”

Section: Xct Characterisation Of Scaffolds and Biomaterialsmentioning

confidence: 99%

Applications of X‐ray computed tomography for the evaluation of biomaterial‐mediated bone regeneration in critical‐sized defects

Fernández

Witte²,

Tozzi

2019

Journal of Microscopy

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Bone as such displays an intrinsic regenerative potential following fracture; however, this capacity is limited with large bone defects that cannot heal spontaneously. The management of critical‐sized bone defects remains a major clinical and socioeconomic need with osteoregenerative biomaterials constantly under development aiming at promoting and enhancing bone healing. X‐ray computed tomography (XCT) has become a standard and essential tool for quantifying structure–function relationships in bone and biomaterials, facilitating the development of novel bone tissue engineering strategies. This paper presents recent advancements in XCT analysis of biomaterial‐mediated bone regeneration. As a noninvasive and nondestructive technique, XCT allows for qualitative and quantitative evaluation of three‐dimensional (3D) scaffolds and biomaterial microarchitecture, bone growth into the scaffold as well as the 3D characterisation of biomaterial degradation and bone regeneration in vitro and in vivo. Furthermore, in combination with in situ mechanical testing and digital volume correlation (DVC), XCT demonstrated its potential to better understand the bone–biomaterial interactions and local mechanics of bone regeneration during the healing process in relation to the regeneration achieved in vivo, which will likely provide valuable knowledge for the development and optimisation of novel osteoregenerative biomaterials. Lay Description Bone, being a dynamically adaptable material, displays excellent regenerative properties following fracture. However, the self‐healing capacity of bone becomes more difficult with large bone defects. Those defects are common and occur in many clinical situations; hence, biomaterials are mostly used to restore both bone structure and function in the defect site. X‐ray computed tomography (XCT) is a powerful tool to evaluate bone regeneration in critical‐sized defects after the implantation of biomaterials, allowing to an improved understanding of the regeneration process following different bone tissue engineering approaches. This paper focuses on recent advancements in XCT analysis to characterise biomaterial‐mediated bone regeneration in critical‐sized defects. XCT supports three‐dimensional (3D) analysis of biomaterials, scaffolds and regenerated bone microarchitecture, as well as bone ingrowth into the scaffold. As a nondestructive technique, XCT allows for a 3D characterisation of biomaterial degradation and bone regeneration over time. In addition, XCT combined with in situ mechanical experiments and digital volume correlation (DVC) provides a 3D evaluation and quantification of bone–biomaterial interactions and deformation mechanisms during the regeneration process. This remains essential for the development and enhancement of novel biomaterials able to produce bone that is comparable with the native tissue they aim to replace.

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Biomaterial granules used for filling bone defects constitute 3D scaffolds: porosity, microarchitecture and molecular composition analyzed by microCT and Raman microspectroscopy

Cited by 26 publications

References 41 publications

Dental alloplastic bone substitutes currently available in Korea

Dental alloplastic bone substitutes currently available in Korea

Bioactivating a bone substitute accelerates graft incorporation in a murine model of vertical ridge augmentation

Applications of X‐ray computed tomography for the evaluation of biomaterial‐mediated bone regeneration in critical‐sized defects

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