Biomaterial screening of protein coatings and peptide additives: towards a simple synthetic mimic of a complex natural coating for a bio-artificial kidney

Gaal, Ronald C. van; Vrehen, Annika F.; Sprang, Johnick F. van; Fransen, Peter; Turnhout, Mark C. van; Dankers, Patricia Y. W.

doi:10.1039/d0bm01930e

Cited by 14 publications

(20 citation statements)

References 52 publications

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“…This study combines material selections, cell types and experimental read-outs from previous studies of our group. 24,26,31,36 The synergistic interactions provide new insights in terms of material behavior and cell specificity, moreover it shows the reproducibility of our previous studies. A small biomaterial library was constructed using two different supramolecular systems (i.e.…”

Section: Introductionsupporting

confidence: 66%

“…UPy-Catechol, UPy-cRGD, BU-Catechol and BU-cRGD were synthesized as reported previously. 26,31,36 PCLdiUPy (M n = 2.8 kg/mol), PriplastdiUPy (M n = 5.0 kg/mol) PCL-BU (M n = 2.7 kg/mol per segment), PC-BU (M n = 2.4 kg/mol per segment) were acquired from SyMO-Chem (The Netherlands). To prepare functionalized polymer films, PCLdiUPy, PCL-BU and PC-BU were dissolved in hexafluoroisopropanol (20 mg/ml; HFIP, Fluorochem) and 5 mol% of corresponding UPy-Catechol, UPy-cRGD, BU-Catechol, or BU-cRGD was added.…”

Section: Polymer Film Preparationmentioning

confidence: 99%

“…24,31 Remarkably, when BU conjugated catechols (BU-Catechol) were incorporated in BU modified PCL (PCL-BU) they enabled long term cell adhesion. 26 Overall, these reports indicate the importance of understanding the influence of the polymer on additive presentation in different supramolecular systems.…”

Section: Introductionmentioning

confidence: 96%

“…36 RPTECs were employed to screen for long-term beneficial effects of BU-peptides and BU-Catechol. 26 Finally, CMPCs adhesion indicated the effectiveness of UPy-Catechol to promote cell adhesion in different UPy-polymers. 24 In this study, cell adhesion, cell spreading and focal adhesion properties were quantified to assess cell response to the materials.…”

Section: Introductionmentioning

confidence: 97%

“…24,25 This enables the construction of biomaterial screening libraries. 25,26 However, the effectiveness of the additive transposition between different supramolecular polymer platforms has been sparsely researched. The nano-scale self-assembly of supramolecular materials has been shown to alter with different polymer backbones, 24,27,28 and additives.…”

Section: Introductionmentioning

confidence: 99%

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Effectiveness of cell adhesive additives in different supramolecular polymers

Gaal

Ippel

Spaans

et al. 2021

Journal of Polymer Science

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Supramolecular motifs in elastomeric biomaterials facilitate the modular incorporation of additives with corresponding motifs. The influence of the elastomeric supramolecular base polymer on the presentation of additives has been sparsely examined, limiting the knowledge of transferability of effective functionalization between polymers. Here it was investigated if the polymer backbone and the additive influence biomaterial modification in two different types of hydrogen bonding supramolecular systems, that is, based on ureido‐pyrimidinone or bis‐urea units. Two different cell‐adhesive additives, that is, catechol or cyclic RGD, were incorporated into different elastomeric polymers, that is, polycaprolactone, priplast or polycarbonate. The additive effectiveness was evaluated with three different cell types. AFM measurements showed modest alterations on nano‐scale assembly in ureido‐pyrimidinone materials modified with additives. On the contrary, additive addition was highly intrusive in bis‐urea materials. Detailed cell adhesive studies revealed additive effectiveness varied between base polymers and the supramolecular platform, with bis‐urea materials more potently affecting cell behavior. This research highlights that additive transposition might not always be as evident. Therefore, additive effectiveness requires re‐evaluation in supramolecular biomaterials when altering the polymer backbone to suit the biomaterial application.

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Section: Introductionsupporting

confidence: 66%

Section: Polymer Film Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effectiveness of cell adhesive additives in different supramolecular polymers

Gaal

Ippel

Spaans

et al. 2021

Journal of Polymer Science

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The Impact of Culture Variables on a 3D Human In Vitro Bone Remodeling Model: A Design of Experiments Approach

Wildt

Cuypers

Cramer

et al. 2023

Adv Healthcare Materials

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Human in vitro bone remodeling models, using osteoclast–osteoblast cocultures, can facilitate the investigation of human bone remodeling while reducing the need for animal experiments. Although current in vitro osteoclast–osteoblast cocultures have improved the understanding of bone remodeling, it is still unknown which culture conditions support both cell types. Therefore, in vitro bone remodeling models can benefit from a thorough evaluation of the impact of culture variables on bone turnover outcomes, with the aim to reach balanced osteoclast and osteoblast activity, mimicking healthy bone remodeling. Using a resolution III fractional factorial design, the main effects of commonly used culture variables on bone turnover markers in an in vitro human bone remodeling model are identified. This model is able to capture physiological quantitative resorption–formation coupling along all conditions. Culture conditions of two runs show promising results: conditions of one run can be used as a high bone turnover system and conditions of another run as a self‐regulating system as the addition of osteoclastic and osteogenic differentiation factors is not required for remodeling. The results generated with this in vitro model allow for better translation between in vitro studies and in vivo studies, toward improved preclinical bone remodeling drug development.

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Off‐the‐Shelf Synthetic Biodegradable Grafts Transform In Situ into a Living Arteriovenous Fistula in a Large Animal Model

Besseling,

Szymczyk,

Teraa

et al. 2024

Adv Healthcare Materials

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Current vascular access options require frequent interventions. In situ tissue engineering may overcome these limitations by combining the initial success of synthetic grafts with the long‐term advantages of autologous vessels by using biodegradable grafts that transform into autologous vascular tissue at the site of implantation. Scaffolds(6mm‐Ø) made of supramolecular polycarbonate‐bisurea(PC‐BU), with a polycaprolactone(PCL) anti‐kinking‐coil, were implanted between the carotid artery and jugular vein in goats. A subset was bio‐functionalized using bisurea‐modified‐SDF1α‐derived peptides and ePTFE grafts as controls. Patency was monitored monthly. Grafts were explanted after 1 and 3 months, and evaluated for material degradation, tissue formation, compliance, and patency. At 3 months, the scaffold was resorbed and replaced by, and vascular neo‐tissue, including elastin, contractile markers an endothelial lining. No dilations, ruptures, or aneurysms were observed and grafts were successfully cannulated at termination. SDF1α‐peptide‐biofunctionalization did not influence outcomes. Patency was lower in the TE grafts(50%) compared to controls(100% patency), predominantly caused by intimal hyperplasia. We demonstrated the rapid remodeling of a synthetic, biodegradable vascular scaffold into a living, compliant arteriovenous fistula in a large animal model. Despite lower patency compared to ePTFE, this transformation into autologous and compliant living tissue with self‐healing capacity may have long‐term advantages.This article is protected by copyright. All rights reserved

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Biomaterial screening of protein coatings and peptide additives: towards a simple synthetic mimic of a complex natural coating for a bio-artificial kidney

Abstract: A bis-urea biomaterial additive library was generated via a DoE approach. Comparison with a protein coating library revealed that simple catechol additives can replace a complex coating to create a living membrane for a bio-artificial kidney.

Cited by 14 publications

References 52 publications

Effectiveness of cell adhesive additives in different supramolecular polymers

Effectiveness of cell adhesive additives in different supramolecular polymers

The Impact of Culture Variables on a 3D Human In Vitro Bone Remodeling Model: A Design of Experiments Approach

Off‐the‐Shelf Synthetic Biodegradable Grafts Transform In Situ into a Living Arteriovenous Fistula in a Large Animal Model

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