Biomaterializing the advances in uterine tissue engineering

Wei, Zhangming; Huiuk, Yi; He, Xiang; Wen, Liping; Li, Zhenjuan; Wang, Qiru; Li, Liping

doi:10.1016/j.isci.2022.105657

Cited by 9 publications

(3 citation statements)

References 125 publications

(142 reference statements)

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“…Microscopy and fluorescence technologies could be used to detect the distribution and concentration of drugs loaded in these bioengineering technologies, promoting the evaluation of cell responsiveness and minor alterations between cells and the microenvironment [ 192 , 193 ]. Undoubtedly, the application and clinical promotion of bioengineering materials used for endometrial diseases for drug screening and testing will eventuate based on the profound understanding of the advanced progress in the field of endometrial bioengineering platforms within recent years [ 55 , 194 ]. Therefore, by studying the existing experimental results, we can conclude that bioengineering platforms are promising tools for pharmacological experiments on endometrial diseases.…”

Section: Application Of Bioengineering Technology In Endometrial Dise...mentioning

confidence: 99%

Bioengineering approaches for the endometrial research and application

Dai,

Liang,

Guo

et al. 2024

Materials Today Bio

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Section: Application Of Bioengineering Technology In Endometrial Dise...mentioning

confidence: 99%

Bioengineering approaches for the endometrial research and application

Dai,

Liang,

Guo

et al. 2024

Materials Today Bio

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“…This includes designing scaffolds for the uterus, which is a promising way to tackle important medical issues related to the uterus, pregnancy, and childbirth. 11 In tissue engineering, biomaterials play an important role by providing a framework similar to the extracellular matrix (ECM) structure. 12 In order to mimic the ECM fibrous structure of tissues, the electrospinning technique is used to create nanofibrous scaffolds.…”

Section: Introductionmentioning

confidence: 99%

“…One such approach involves tissue engineering using principles to create new scaffolds tailored to specific tissues is essential, which has been explored in this study to design a patch to treat the region of scar or wound in the myometrium. This includes designing scaffolds for the uterus, which is a promising way to tackle important medical issues related to the uterus, pregnancy, and childbirth . In tissue engineering, biomaterials play an important role by providing a framework similar to the extracellular matrix (ECM) structure .…”

Section: Introductionmentioning

confidence: 99%

Surface-Conjugated Galactose on Electrospun Polycaprolactone Nanofibers: An Innovative Scaffold for Uterine Tissue Engineering

Hanuman,

Pai

et al. 2024

ACS Omega

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The uterus, a vital organ in the female reproductive system, nurtures and supports developing embryos until maturity. This study focuses on addressing uterine related problems by creating a nanofibrous scaffold to regenerate uterine myometrial tissue, closely resembling the native extracellular matrix (ECM) for enhanced efficacy. To achieve this, we utilized polycaprolactone (PCL) as a biomaterial and employed an electrospinning technique to generate PCL nanofibers in both random and aligned orientations. Due to the inherent hydrophobic nature of PCL nanofibers, a two-step wet chemistry surface modification technique is used, involving the conjugation of galactose onto them. Galactose, a lectin-binding sugar, was chosen to enhance the scaffold's hydrophilicity, thereby improving cell adhesion and fostering L-selectin-based interactions between the scaffold and uterine cells. These interactions, in turn, activated uterine fibroblasts, leading to ECM remodeling. The optimized electrospinning process successfully generated random and aligned nanofibers. Subsequent surface modification was carried out, and the modified scaffold was subjected to various physicochemical characterization, such as the ninhydrin assay, enzyme-linked lectin assay techniques that revealed successful galactose conjugation, and mechanical characterization to assess any changes in material bulk properties resulting from the modification. The tensile strength of random galactose-modified PCL fibers reached 0.041 ± 0.01 MPa, outperforming random unmodified PCL fibers (0.026 ± 0.01 MPa), aligned unmodified PCL fibers (0.011 ± 0.001 MPa), and aligned modified PCL fibers (0.016 ± 0.002 MPa). Cytocompatibility studies with human uterine fibroblast cells showed enhanced viability and proliferation on the modified scaffolds. Initial pilot studies were attempted in the current study involving subcutaneous implantation in the dorsal area of Wistar rats to assess biocompatibility and tissue response before proceeding to intrauterine implantation indicated that the modification did not induce adverse inflammation in vivo. In conclusion, our study introduces a surface-modified PCL nanofibrous material for myometrial tissue engineering, offering promise in addressing myometrial damage and advancing uterine health and reproductive well-being.

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Multifunctional group mediated double cross-linked polysaccharide complex hydrogel for microenvironmental regulation and repair of endometrial injury

Yang,

Liu,

et al. 2024

Chemical Engineering Journal

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Biomaterializing the advances in uterine tissue engineering

Cited by 9 publications

References 125 publications

Bioengineering approaches for the endometrial research and application

Bioengineering approaches for the endometrial research and application

Surface-Conjugated Galactose on Electrospun Polycaprolactone Nanofibers: An Innovative Scaffold for Uterine Tissue Engineering

Multifunctional group mediated double cross-linked polysaccharide complex hydrogel for microenvironmental regulation and repair of endometrial injury

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