Biomaterials derived from silk–tropoelastin protein systems

Hu, Xiao; Wang, Xiuli; Rnjak, Jelena; Weiss, Anthony S.; Kaplan, David L.

doi:10.1016/j.biomaterials.2010.07.044

Cited by 148 publications

(211 citation statements)

References 49 publications

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“…This difference is significant (p <0.01). According to the literature, the region between 1600 and 1640 cm -1 is related to the presence of β sheets 48 . The signals that appear in the region 1640-1660 cm -1 are associated with the presence of random coils and α-helices.…”

Section: Molecular Level: Ftir Analysismentioning

confidence: 99%

“…The signals that appear in the region 1640-1660 cm -1 are associated with the presence of random coils and α-helices. The remaining parts of the spectra (1660-1690) are dominated by vibrations due to β-turn structures 48 . Consequently, the observed shift in frequency between the control and annealed samples is indicative of the formation of β-sheet structures 48 .…”

Section: Molecular Level: Ftir Analysismentioning

confidence: 99%

See 1 more Smart Citation

Self-Organized ECM-Mimetic Model Based on an Amphiphilic Multiblock Silk-Elastin-Like Corecombinamer with a Concomitant Dual Physical Gelation Process

et al. 2014

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ABSTRACT. Although significant progress has been made in the area of injectable hydrogels for biomedical applications and model cell niches, further improvements are still needed, especially in terms of mechanical performance, stability and biomimicry of the native fibrillar architecture found in the extracellular matrix (ECM). This work focuses on the design and production of a silk-elastin-based injectable multiblock co-recombinamer that spontaneously forms a stable physical nanofibrillar hydrogel under physiological conditions. That differs from previously reported silk-elastin-like polymers on a major content and predominance of the elastin-like part, as well as a more complex structure and behavior of such part of the molecule, which is aimed to obtain well defined hydrogels.Rheological and DSC experiments showed that this system displays a coordinated and concomitant dual gelation mechanism. In a first stage, a rapid, thermally driven gelation of the co-recombinamer solution takes place once the system reaches body temperature due to the thermal responsiveness of the elastinlike (EL) parts and the amphiphilic multiblock design of the co-recombinamer. A bridged micellar 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 2 structure is the dominant microscopic feature of this stage, as demonstrated by AFM and TEM.Completion of the initial stage triggers the second, which comprises a stabilization, reinforcement, and microstructuring of the gel. FTIR analysis shows that these events involve the formation of β-sheets around the silk motifs. The emergence of such β-sheet structures leads to the spontaneous selforganization of the gel into the final fibrous structure. Despite the absence of biological cues, here we set the basis of the minimal structure that is able to display such a set of physical properties and undergo microscopic transformation from a solution to a fibrous hydrogel. The results point to the potential of this system as a basis for the development of injectable fibrillar biomaterial platforms towards a fully functional, biomimetic, artificial extracellular matrix and cell niches.

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Section: Molecular Level: Ftir Analysismentioning

confidence: 99%

Section: Molecular Level: Ftir Analysismentioning

confidence: 99%

Self-Organized ECM-Mimetic Model Based on an Amphiphilic Multiblock Silk-Elastin-Like Corecombinamer with a Concomitant Dual Physical Gelation Process

et al. 2014

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“…15,16 Gelation of silk fibroin protein solution can be induced by sonication, physical agitation, alcohol dehydration, and electrical current. [17][18][19] In addition, the physical properties of silk materials can be tuned to meet functional demands by blending with other macromolecules such as tropoelastin (increased elasticity) 20 or hyaluronan (biological signaling). 21 We recently described a 2-part, silk-polyethylene glycol (PEG) biomaterial for use as a tissue sealant.…”

Section: Introductionmentioning

confidence: 99%

644: A silk-based injectable biomaterial as an alternative to cervical cerclage: an in-vitro study

Heard

Socrate

Burke

et al. 2012

American Journal of Obstetrics and Gynecology

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“…For example, the radial alignment of SMC in the arterial media is mimicked by culture of these cells on parallel synthetic elastin fibers (Nivison-Smith & Weiss 2011, submitted). Blended conduits of synthetic elastin and silk or polycaprolactone display elasticity and cell adhesion properties courtesy of the rhTE component while the composite component confers additional mechanical strength (Hu, Wang et al 2010;Wise, Byrom et al 2011). …”

Section: Recombinant Human Tropoelastinmentioning

confidence: 99%