Biomaterials in Cell Microencapsulation

Santos, Edorta; Zárate, Jon; Orive, Gorka; Hernández, Rosa María; Pedraz, José Luís

doi:10.1007/978-1-4419-5786-3_2

Cited by 76 publications

(45 citation statements)

References 150 publications

(171 reference statements)

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“…One strategy for preventing cellmediated rejection is to place cells within immunoisolation devices such as microcapsules made from various polymers including agarose, polyethylene glycol, polyacrylates, and alginate [26]. Alginates are the most commonly used polymers for microencapsulation because of their biocompatibility and the ability to cross-link with Ca 2 + / Ba 2 + at physiological pH [27]. Barium alginate microcapsules have a diameter ranging from 100 to 700 mm and a pore size of *250 kDa, thereby allowing the diffusion of nutrients and oxygen, but preventing the entry of immune cells and antibodies [28,29].…”

Section: Introductionmentioning

confidence: 99%

Hepatocyte-Like Cells Derived from Human Amniotic Epithelial Cells Can Be Encapsulated Without Loss of Viability or Function In Vitro

Vaghjiani

Vaithilingam

Saraswati

et al. 2014

Stem Cells and Development

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Placenta derived human amniotic epithelial cells (hAEC) are an attractive source of stem cells for the generation of hepatocyte-like cells (HLC) for therapeutic applications to treat liver diseases. During hAEC differentiation into HLC, they become increasingly immunogenic, which may result in immune cell-mediated rejection upon transplantation into allogeneic recipients. Placing cells within devices such as alginate microcapsules can prevent immune cell-mediated rejection. The aim of this study was to investigate the characteristics of HLC generated from hAEC and to examine the effects of encapsulation on HLC viability, gene expression, and function. hAEC were differentiated for 4 weeks and evaluated for hepatocyte-specific gene expression and function. Differentiated cells were encapsulated in barium alginate microcapsules and cultured for 7 days and the effect of encapsulation on cell viability, function, and hepatocyte related gene expression was determined. Differentiated cells performed key functions of hepatocytes including urea synthesis, drug-metabolizing cytochrome P450 (CYP)3A4 activity, indocyanine green (ICG) uptake, low-density lipoprotein (LDL) uptake, and exhibited glutathione antioxidant capacity. A number of hepatocyte-related genes involved in fat, cholesterol, bile acid synthesis, and xenobiotic metabolism were also expressed showing that the hAEC had differentiated into HLC. Upon encapsulation, the HLC remained viable for at least 7 days in culture, continued to express genes involved in fat, cholesterol, bile acid, and xenobiotic metabolism and had glutathione antioxidant capacity. CYP3A4 activity and urea synthesis by the encapsulated HLC were higher than that of monolayer HLC cultures. Functional HLC can be derived from hAEC, and HLC can be encapsulated within alginate microcapsules without losing viability or function in vitro.

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Section: Introductionmentioning

confidence: 99%

Hepatocyte-Like Cells Derived from Human Amniotic Epithelial Cells Can Be Encapsulated Without Loss of Viability or Function In Vitro

Vaghjiani

Vaithilingam

Saraswati

et al. 2014

Stem Cells and Development

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“…This has made alginate the material of choice for microencapsulation of cells, in which easily available and inexpensive sodium alginate, which is unmodified, is quickly rearranged into calcium alginate hydrogel microspheres, held together through ionic interactions. 73 These constructs have been extensively used for creating hydrogel capsules containing trapped liver cells or pancreatic islets. 66 However, without chemical modification, alginate, like PEG, is mostly inert, and its use for cell and tissue culture is limited without incorporating cell-adherent motifs.…”

Section: Gelatinmentioning

confidence: 99%

Biomaterials for Integration with 3-D Bioprinting

2014

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Bioprinting has emerged in recent years as an attractive method for creating 3-D tissues and organs in the laboratory, and therefore is a promising technology in a number of regenerative medicine applications. It has the potential to (i) create fully functional replacements for damaged tissues in patients, and (ii) rapidly fabricate small-sized human-based tissue models, or organoids, for diagnostics, pathology modeling, and drug development. A number of bioprinting modalities have been explored, including cellular inkjet printing, extrusion-based technologies, soft lithography, and laser-induced forward transfer. Despite the innovation of each of these technologies, successful implementation of bioprinting relies heavily on integration with compatible biomaterials that are responsible for supporting the cellular components during and after biofabrication, and that are compatible with the bioprinting device requirements. In this review, we will evaluate a variety of biomaterials, such as curable synthetic polymers, synthetic gels, and naturally derived hydrogels. Specifically we will describe how they are integrated with the bioprinting technologies above to generate bioprinted constructs with practical application in medicine.

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“…Moreover, divalent cations with a high binding constant with alginate can enhance the strength of the hydrogel, which provides another way to tune the mechanical properties of the alginate hydrogel. Alginate hydrogel has been used widely as a biomaterial for encapsulation of cells [31,53].…”

Section: Protein-based Hydrogelmentioning

confidence: 99%

Microfluidic-Based Synthesis of Hydrogel Particles for Cell Microencapsulation and Cell-Based Drug Delivery

2012

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Encapsulation of cells in hydrogel particles has been demonstrated as an effective approach to deliver therapeutic agents. The properties of hydrogel particles, such as the chemical composition, size, porosity, and number of cells per particle, affect cellular functions and consequently play important roles for the cell-based drug delivery. Microfluidics has shown unparalleled advantages for the synthesis of polymer particles and been utilized to produce hydrogel particles with a well-defined size, shape and morphology. Most importantly, during the encapsulation process, microfluidics can control the number of cells per particle and the overall encapsulation efficiency. Therefore, microfluidics is becoming the powerful approach for cell microencapsulation and construction of cell-based drug delivery systems. In this article, I summarize and discuss microfluidic approaches that have been developed recently for the synthesis of hydrogel particles and encapsulation of cells. I will start by classifying different types of hydrogel material, including natural biopolymers and synthetic polymers that are used for cell encapsulation, and then focus on the current status and challenges of microfluidic-based approaches. Finally, applications of cell-containing hydrogel particles for cell-based drug delivery, particularly for cancer therapy, are discussed.

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Biomaterials in Cell Microencapsulation

Cited by 76 publications

References 150 publications

Hepatocyte-Like Cells Derived from Human Amniotic Epithelial Cells Can Be Encapsulated Without Loss of Viability or Function In Vitro

Hepatocyte-Like Cells Derived from Human Amniotic Epithelial Cells Can Be Encapsulated Without Loss of Viability or Function In Vitro

Biomaterials for Integration with 3-D Bioprinting

Microfluidic-Based Synthesis of Hydrogel Particles for Cell Microencapsulation and Cell-Based Drug Delivery

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