Biomathematical pattern of EMG signal propagation in smooth muscle of the non-pregnant porcine uterus

Abstract

Uterine contractions are generated by myometrial smooth muscle cells (SMCs) that comprise most of the myometrial layer of the uterine wall. Aberrant uterine motility (i.e., hypo- or hyper-contractility or asynchronous contractions) has been implicated in the pathogenesis of infertility due to the failure of implantation, endometriosis and abnormal estrous cycles. The mechanism whereby the non-pregnant uterus initiates spontaneous contractions remains poorly understood. The aim of the present study was to employ linear synchronization measures for analyzing the pattern of EMG signal propagation (direction and speed) in smooth muscles of the non-pregnant porcine uterus in vivo using telemetry recording system. It has been revealed that the EMG signal conduction in the uterine wall of the non-pregnant sow does not occur at random but it rather exhibits specific directions and speed. All detectable EMG signals moved along the uterine horn in both cervico-tubal and tubo-cervical directions. The signal migration speed could be divided into the three main types or categories: i. slow basic migration rhythm (SBMR); ii. rapid basic migration rhythm (RBMR); and iii. rapid accessory migration rhythm (RAMR). In conclusion, the EMG signal propagation in smooth muscles of the porcine uterus in vivo can be assessed using a linear synchronization model. Physiological pattern of the uterine contractile activity determined in this study provides a basis for future investigations of normal and pathologicall myogenic function of the uterus.

Characteristics of bioelectrical activity of oviducts and uterus during early pregnancy in sows recorded by telemetry method

“…The frequency, amplitude and duration of contractions are determined mainly by the frequency of occurrence of the uterine electrical bursts, the total number of cells that are simultaneously active during the bursts and the duration of the uterine electrical bursts, respectively (Garfield & Maner, ; Domino et al . , ; Pawlinski et al . ).…”

“…In research conducted with the use of telemetry, the surgical technique, the materials used and the design of the electrodes are of vital importance. Many published studies focus on non-pregnant, cycling uterine contractile activity in the rat (Ishikawa & Fuchs, 1978;Downing et al 1981;Crane & Martin, 1991a,b), guinea-pig (Chernaeva & Milenov, 1974), dog (Wheaton et al 1988), cow (Ruckebusch & Bayard, 1975;Hirsbrunner et al 2002Hirsbrunner et al , 2010Kaufmann et al 2008), horse (Hirsbrunner et al 2006) and sow (Mwanza et al 2002;Domino et al 2016Domino et al , 2017aPawlinski et al 2016). The induction and synchronization of oestrus are the most prevalent procedures performed to manage the timing of oestrus to meet the needs of the farm.…”

“…Studies on isolated myometrial tissue have demonstrated the connection between electrical events and contractions (Garfield & Maner, 2007). The frequency, amplitude and duration of contractions are determined mainly by the frequency of occurrence of the uterine electrical bursts, the total number of cells that are simultaneously active during the bursts and the duration of the uterine electrical bursts, respectively (Garfield & Maner, 2007;Domino et al 2016Domino et al , 2017aPawlinski et al 2016). Factors that directly stimulate or inhibit uterine contractions do so by altering the electrical properties and the excitability or conductivity of myometrial cells (Garfield & Maner, 2007).…”

Anti-Nogo-A Antibodies As a Potential Causal Therapy for Lower Urinary Tract Dysfunction after Spinal Cord Injury

“…High-frequency activity was low during voiding (Figs. 1j, 2a,d), whereas in contrast, slow-wave bursting activity (2-20 Hz, representing mainly smooth muscle activity; Domino et al, 2017) was prominent (Figs. 1j, 2a,d).…”

Automatic Segmentation Scheme for Effective Synchronization of EMG-EEG Quantification

“…The actual EMG signal originating from muscle activities can degrade due to the mixing noise or artifacts [13,17,18]. These noises could arise from several factors, such as motion artifact due to muscle and electrodes movement concerning one another and the power line interference.…”