Biomatrices for bladder reconstruction

Lin, Hsueh Kung; Madihally, Sundararajan V.; Palmer, Blake; Frimberger, Dominic; Fung, Kar Ming; Kropp, Bradley P.

doi:10.1016/j.addr.2014.11.020

Cited by 63 publications

(60 citation statements)

References 201 publications

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“…In particular, urinary bladder and lung matrices, arteries and heart valves from allogenic human sources have been applied in preclinical and clinical procedures over the last decade, although the outcomes of large-animal models and human clinical trials have varied (Table 2) (reviewed in Refs. [26,76,266,298,477,520,521]). However, we must be mindful of the fact that our understanding of the cellular processes involved in the recellularization and revitalization of these bioscaffolds to form practical tissues is still incomplete [522].…”

Section: Human Tissue Ecm-based Biomaterialsmentioning

confidence: 99%

“…All of these inherent advantages have sparked strategies that apply SIS-ECM as a scaffolding material in applications for soft-tissue reconstruction and cardiovascular, neural and urogenital tissue engineering [39]. SIS is currently approved by the FDA for several urogenital uses, including bladder augmentation, hernia repair and urethral stricture repair [26,76,77]. Although SIS-ECM is evolving and slowly becoming a new standard for bladder augmentation [269], collagen-composite biomaterials that consist of nanofiber networks highly resembling natural ECMs populated with self-assembled fibroblast sheets or the patient's own muscle and urothelial cells are another attractive strategy that is showing optimistic clinical outcomes [74,524,525].…”

Section: Human Tissue Ecm-based Biomaterialsmentioning

confidence: 99%

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Advancing biomaterials of human origin for tissue engineering

Chen

Liu

2016

Progress in Polymer Science

959

513

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Biomaterials have played an increasingly prominent role in the success of biomedical devices and in the development of tissue engineering, which seeks to unlock the regenerative potential innate to human tissues/organs in a state of deterioration and to restore or reestablish normal bodily function. Advances in our understanding of regenerative biomaterials and their roles in new tissue formation can potentially open a new frontier in the fast-growing field of regenerative medicine. Taking inspiration from the role and multi-component construction of native extracellular matrices (ECMs) for cell accommodation, the synthetic biomaterials produced today routinely incorporate biologically active components to define an artificial in vivo milieu with complex and dynamic interactions that foster and regulate stem cells, similar to the events occurring in a natural cellular microenvironment. The range and degree of biomaterial sophistication have also dramatically increased as more knowledge has accumulated through materials science, matrix biology and tissue engineering. However, achieving clinical translation and commercial success requires regenerative biomaterials to be not only efficacious and safe but also cost-effective and convenient for use and production. Utilizing biomaterials of human origin as building blocks for therapeutic purposes has provided a facilitated approach that closely mimics the critical aspects of natural tissue with regard to its physical and chemical properties for the orchestration of wound healing and tissue regeneration. In addition to directly using tissue transfers and transplants for repair, new applications of human-derived biomaterials are now focusing on the use of naturally occurring biomacromolecules, decellularized ECM scaffolds and autologous preparations rich in growth factors/non-expanded stem cells to either target acceleration/magnification of the body's own repair capacity or use nature's paradigms to create new tissues for restoration. In particular, there is increasing interest in separating ECMs into simplified functional domains and/or biopolymeric assemblies so that these components/constituents can be discretely exploited and manipulated for the production of bioscaffolds and new biomimetic biomaterials. Here, following an overview of tissue auto-/allo-transplantation, we discuss the recent trends and advances as well as the challenges and future directions in the evolution and application of human-derived biomaterials for reconstructive surgery and tissue engineering. In particular, we focus on an exploration of the structural, mechanical, biochemical and biological information present in native human tissue for bioengineering applications and to provide inspiration for the design of future biomaterials.

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Section: Human Tissue Ecm-based Biomaterialsmentioning

confidence: 99%

Section: Human Tissue Ecm-based Biomaterialsmentioning

confidence: 99%

Advancing biomaterials of human origin for tissue engineering

Chen

Liu

2016

Progress in Polymer Science

959

513

View full text Add to dashboard Cite

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“…However, the lack of cytokines and growth factors that support and induce the growth and differentiation of cells restricts these synthetic polymers. 28 By contrast, at least 10 growth factors, including VEGF, BMP4, PDGF-BB, KGF, TGFbeta1, IGF, bFGF, EGF, and TGFalpha were found to be preserved in the BAM. 9 These active proteins can provide an excellent microenvironment to orchestrate growth, migration, proliferation, and differentiation of cells.…”

Section: Discussionmentioning

confidence: 99%

Tissue-engineered tubular substitutions for urinary diversion in a rabbit model

Meng

Liao

Yang

et al. 2015

Exp Biol Med (Maywood)

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Clinically, autologous gastrointestinal segments are traditionally used for urinary diversion. However, this procedure often causes many serious complications. Tissue engineering may provide an alternative treatment method in urinary diversion. This research aims to produce tissue-engineered tubular substitutions by using homologous adipose-derived stem cells, smooth muscle cells, and bladder acellular matrix in developing urinary diversion in a rabbit model. Adipose-derived stem cells and smooth muscle cells of rabbit were obtained and cultured in vitro. These cultured adipose-derived stem cells and smooth muscle cells were seeded onto the two sides of the bladder acellular matrix and then incubated for seven days. The cell-seeded matrix was used to build tissue-engineered tubular substitutions, which were then implanted and wrapped into the omentum in vivo for two weeks to promote angiogenesis. In the experimental group, the bladder of 20 rabbits was totally resected, and the above tissue-engineered tubular substitutions were used for urinary diversion. In the control group, bladder acellular matrix tubular substitutions with unseeded cells were implanted into the omentum and were used as urinary diversion on another five rabbits with the same process. The implants were harvested, and histological examination was conducted at 2, 4, 8, and 16 weeks after operation. Intravenous urography assessment was performed at 16 weeks postoperatively. All the rabbits were alive in the experimental group until they were sacrificed. Histological analysis of the construct displayed the presence of multilayer urothelial cells on the luminal side and organized smooth muscle tissue on the other side, and different diameters of neovascularization were clearly identified in the substitutions obtained. No leakage, stricture, or obstructions were noted with intravenous urography assessment. All the animals in the control group died within two weeks, and urine leakage, scar formation, and inflammation were detected through autopsy. This study demonstrates the feasibility of tissue-engineered tubular substitutions constructed using homologous adipose-derived stem cells, smooth muscle cells, and bladder acellular matrix for urinary diversion in a rabbit model.

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“…Unlike the acidic remnants produced by degrading synthetic polymers of PGA/PLGA, the bilayer chitosan scaffold is metabolized into non-toxic D-glucosamines by lysozymes and tuned with suitable degradation speed for bladder repair, which alleviates the systematic toxicity and the local inammation reaction, and eliminates the risk of nidus for large bladder calculus. 28 Although a small quantity of bladder calculus was induced by the chitosan scaffold, it facilitated bladder regeneration with trivial scaffold shrinkage, brosis and metabolic disturbance.…”

Section: Characteristics Of the Animal Modelsmentioning

confidence: 99%

Comparison of morphological and functional restoration between asymmetric bilayer chitosan and bladder acellular matrix graft for bladder augmentation in a rat model

Xiao

Wang

et al. 2017

RSC Adv.

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Because of the limitations of current scaffolds and unfavorable results of clinical trials, proper scaffolds facilitating bladder reconstruction are highly desirable. The aim of this study was to evaluate a novel asymmetric bilayer chitosan scaffold compared with conventional bladder acellular matrix graft (BAMG) in a rat model of bladder augmentation. Twenty-four 8 week-old male Sprague-Dawley rats were randomly assigned to the chitosan scaffold, BAMG and cystotomy groups. The rats' bladders were sampled for cystography and routine histological examination at 21 and 70 days. Immunofluorescence photometry, conscious cystometry, quantitative real-time polymerase chain reaction, and western blot analyses were performed using bladders at 70 days. Compared with BAMG, the chitosan scaffold consisted of a membrane-like compact layer and a sponge-like porous layer with an excellent combination of mechanical strength and flexibility. The chitosan group showed better performances than the BAMG group in radiographic cystography, smooth muscle regeneration, blood vessel numbers and functional restoration. In contrast to reduced bladder compliance induced by BAMG, bladder augmented by chitosan displayed nearly 1.5-fold increased bladder capacity with comparable compliance to that of the cystotomy group at 70 days. The chitosan group exhibited higher levels of VEGF and VEGFR2, associated with the activation of the hypoxia-related SDF-1a/CXCR4 pathway. These results suggested that the asymmetric bilayer chitosan scaffold is a promising scaffold for bladder reconstruction.

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Biomatrices for bladder reconstruction

Cited by 63 publications

References 201 publications

Advancing biomaterials of human origin for tissue engineering

Advancing biomaterials of human origin for tissue engineering

Tissue-engineered tubular substitutions for urinary diversion in a rabbit model

Comparison of morphological and functional restoration between asymmetric bilayer chitosan and bladder acellular matrix graft for bladder augmentation in a rat model

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