Biomechanical Analysis of the Effect of Mesenchymal Stem Cells on Mandibular Distraction Osteogenesis

Aykan, Andaç; Öztürk, Serdar; Şahin, İsmail; Gürses, Senih; Ural, Ali Uğur; Ören, Nisa Cem; Işık, Selçuk

doi:10.1097/scs.0b013e31827c8706

Cited by 26 publications

(29 citation statements)

References 17 publications

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“…In both long bone [15]–[18] and mandibular DO studies [19]–[21], to date the most commonly used method of cell delivery is direct injection of MSCs carried by platelet rich plasma, saline or collagen gel. While an injection method sounds straightforward, the difficulty involved in delivering the cells to the desired locations of a closed subcutaneous bone site cannot be overestimated.…”

Section: Introductionmentioning

confidence: 99%

Scaffold-Based Delivery of Autologous Mesenchymal Stem Cells for Mandibular Distraction Osteogenesis: Preliminary Studies in a Porcine Model

et al. 2013

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PurposeBone regeneration through distraction osteogenesis (DO) is promising but remarkably slow. To accelerate it, autologous mesenchymal stem cells have been directly injected to the distraction site in a few recent studies. Compared to direct injection, a scaffold-based method can provide earlier cell delivery with potentially better controlled cell distribution and retention. This pilot project investigated a scaffold-based cell-delivery approach in a porcine mandibular DO model.Materials and MethodsEleven adolescent domestic pigs were used for two major sets of studies. The in-vitro set established methodologies to: aspirate bone marrow from the tibia; isolate, characterize and expand bone marrow-derived mesenchymal stem cells (BM-MSCs); enhance BM-MSC osteogenic differentiation using FGF-2; and confirm cell integration with a gelatin-based Gelfoam scaffold. The in-vivo set transplanted autologous stem cells into the mandibular distraction sites using Gelfoam scaffolds; completed a standard DO-course and assessed bone regeneration by macroscopic, radiographic and histological methods. Repeated-measure ANOVAs and t-tests were used for statistical analyses.ResultsFrom aspirated bone marrow, multi-potent, heterogeneous BM-MSCs purified from hematopoietic stem cell contamination were obtained. FGF-2 significantly enhanced pig BM-MSC osteogenic differentiation and proliferation, with 5 ng/ml determined as the optimal dosage. Pig BM-MSCs integrated readily with Gelfoam and maintained viability and proliferative ability. After integration with Gelfoam scaffolds, 2.4–5.8×107 autologous BM-MSCs (undifferentiated or differentiated) were transplanted to each experimental DO site. Among 8 evaluable DO sites included in the final analyses, the experimental DO sites demonstrated less interfragmentary mobility, more advanced gap obliteration, higher mineral content and faster mineral apposition than the control sites, and all transplanted scaffolds were completely degraded.ConclusionIt is technically feasible and biologically sound to deliver autologous BM-MSCs to the distraction site immediately after osteotomy using a Gelfoam scaffold to enhance mandibular DO.

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Section: Introductionmentioning

confidence: 99%

Scaffold-Based Delivery of Autologous Mesenchymal Stem Cells for Mandibular Distraction Osteogenesis: Preliminary Studies in a Porcine Model

et al. 2013

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“…The similarity of the sheep mandible to the human mandible consists of not only anatomic or macroscopic properties but also physiologic properties. 25,26 The physiologic similarities of the sheep mandible to the human mandible, in terms of distraction, have been revealed by Knabe et al 25 Growth factor expression after clinical mandibular DO in humans and its comparison with existing animal studies were reported by Knabe et al, 25 and the authors found that the expression pattern of growth factors secondary to human DO closely resembled those observed in the sheep model. This similarity was also mentioned in studies performed by Ploder et al 13 and Ayoub et al 22 Because of all these similarities, the sheep mandible model was selected in our study.…”

Section: Discussionmentioning

confidence: 72%

Mandibular Distraction Osteogenesis With Newly Designed Electromechanical Distractor

Aykan¹,

Ugurlutan²,

Zor³

et al. 2014

Journal of Craniofacial Surgery

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In this preliminary study, a newly designed electromechanical distractor was successfully used for mandible distraction, which mainly provided a continuous lengthening during activation period spontaneously without any intervention. We think that the clinical application of this electromechanic distractor may provide patient comfort during distraction. Moreover, electromechanical distractor has the potential for high-resolution movement capacity when compared with annual distraction. The promising results from this prototype are encouraging to further investigations for human applications.

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“…. Manual selection following the inclusion criteria resulted in a total of 19 studies as summarized in Table .…”

Section: Resultsmentioning

confidence: 99%

Mandibular distraction osteogenesis assisted by cell‐based tissue engineering: a systematic review

Tee

Sun

2015

Orthod Craniofacial Res

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Objectives To review the advances and limitations of recent investigations on mandibular distraction osteogenesis (MDO) assisted by mesenchymal stem cell (MSC) transplantation. Materials and Methods Following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines, the PubMed, SCOPUS, and Cochrane electronic databases were systematically searched and screened from their inception through August 2014. Searching terms included: ‘distraction osteogenesis’, ‘mandible or mandibular or jaw’, and ‘cells’, without any other limitations. Results Nineteen studies meeting the eligibility criteria were selected from 227 published articles and used for qualitative synthesis. Fifteen of the studies used small animal models (rats or rabbits), while the other 4 used large animal models (dogs, pigs or sheep). Among these studies, large variations exist in MDO protocol, cell transplantation time, route and quantity, as well as methodology of outcome assessment. Additionally, all studies had certain biases. Nevertheless, the majority of studies found that MSC transplantation enhanced MDO bone regeneration. Conclusion Evidence from animal studies indicate that MDO may be enhanced by mesenchymal stem cell transplantation, but many questions related to animal models, MDO protocols, and cell transplantation remain to be investigated.

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Biomechanical Analysis of the Effect of Mesenchymal Stem Cells on Mandibular Distraction Osteogenesis

Abstract: Transplantation of mesenchymal stem cells promotes maturity of the distracted callus. The new experimental model, which allowed to test the mandible as a system by simulating in vivo loading conditions, revealed differences in the mechanical behavior of the halves of mandible.

Cited by 26 publications

References 17 publications

Scaffold-Based Delivery of Autologous Mesenchymal Stem Cells for Mandibular Distraction Osteogenesis: Preliminary Studies in a Porcine Model

Scaffold-Based Delivery of Autologous Mesenchymal Stem Cells for Mandibular Distraction Osteogenesis: Preliminary Studies in a Porcine Model

Mandibular Distraction Osteogenesis With Newly Designed Electromechanical Distractor

Mandibular distraction osteogenesis assisted by cell‐based tissue engineering: a systematic review

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