Biomechanical and histological comparison between the cryopreserved and the lyophilized Gracilis tendon allograft for MPFL reconstruction, a cadaveric experimental study

Negrín, Roberto; Duboy, Jaime; Olavarría, Fernando; Wainer, Mauricio; Jimenez, H. Tellez; Heras, Facundo Las; Reyes, Nicolás O.; Godoy, Hugo

doi:10.1186/s40634-016-0056-2

Cited by 7 publications

(10 citation statements)

References 20 publications

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“…McNeilan et al 22 showed in a recent publication that there is no biomechanical difference in the use of auto or allograft, justified in the low resistance (217 N) of the native MPFL and it would be enough to have a graft 240 mm long and 5 to 6 mm diameter for proper reconstruction. Regarding the use of cryopreserved or lyophilized allograft, Negrín et al 23 in 2016 demonstrated in a study, conducted on 36 swine knees, that there would be no difference in maximum tensile resistance, elongation, area of failure, graft strength or histology.…”

Section: Discussionmentioning

confidence: 99%

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Dynamic–Anatomical Reconstruction of Medial Patellofemoral Ligament in Open Physis

Negrín

Reyes

Iñiguez

et al. 2020

Arthroscopy Techniques

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Patellar dislocation is a common knee problem, 10 times more frequent in childhood and adolescence. Medial patellofemoral ligament is injured up to 94% of the time, and its reconstruction is effective in terms of stabilization of the patella. However, distal femoral physis can be damaged with different techniques of reconstruction, due to the location of the femoral footprint. The purpose of this Technical Note is to describe a quasi-anatomical and dynamic reconstruction of the medial patellofemoral ligament, using no tunnel in the femur, passing the graft behind the adductor tendon, and fixing it with one tunnel in the patella and one passage through the quadriceps tendon.

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Section: Discussionmentioning

confidence: 99%

“…Regarding the use of cryopreserved or lyophilized allograft, Negrín et al. 23 in 2016 demonstrated in a study, conducted on 36 swine knees, that there would be no difference in maximum tensile resistance, elongation, area of failure, graft strength or histology.…”

Section: Discussionmentioning

confidence: 99%

Dynamic–Anatomical Reconstruction of Medial Patellofemoral Ligament in Open Physis

Negrín

Reyes

Iñiguez

et al. 2020

Arthroscopy Techniques

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“…The porcine patella was chosen because it has also been well established in similar biomechanical models. 11 , 15 , 22 In this model, we also used tissue that was not irradiated or sterilized in any way. It has been demonstrated that higher doses of irradiation can affect biomechanical properties of soft-tissue allografts, and even low-dose irradiation may affect the stiffness.…”

Section: Discussionmentioning

confidence: 99%

“…Porcine patellae were used as this is an established and described model of MPFL reconstruction. 11 , 15 , 22 The tendons were not sterilized using irradiation or other techniques. The patellae and tendons were stored at −20°C until 5 hours before testing.…”

Section: Methodsmentioning

confidence: 99%

Cyclic Testing of 3 Medial Patellofemoral Ligament Reconstruction Techniques

Mehta

Akhter

Mandala

2017

Orthopaedic Journal of Sports Medicine

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Background:Several techniques are available to secure the graft to the patella during medial patellofemoral ligament (MPFL) reconstruction. The biomechanical properties of these techniques remain unknown.Purpose:To compare the biomechanical properties of 3 MPFL patellar fixation techniques: bone tunnels (BT), PushLock anchors (PL), and tenodesis screws (TS).Study Design:Controlled laboratory study.Methods:Forty-five MPFL reconstructions were performed using 3 different reconstruction techniques (BT, PL, and TS). The specimens were randomly assigned, with 15 specimens in each of the 3 groups. Cyclic loading (500 cycles) and load-to-failure testing were performed. Gap formation after 100 and 500 cycles, ultimate load to failure, and stiffness were measured.Results:Six constructs failed during cyclic loading, 5 in the PL group (33%) and 1 in the TS group (6.7%). After 100 cycles, differences in gap formation were found between the PL and BT groups (4.48 vs 3.62 mm, P < .03) and between the PL and TS groups (4.48 vs 2.28 mm, P < .0001). After 500 cycles, differences in gap formation were found between the BT and TS groups (6.63 vs 4.16 mm, P < .002) and between the PL and TS groups (7.89 vs 4.16 mm, P < .005). The PL group was found to have a lower ultimate load to failure when compared with the BT group (161.4 vs 258.3 N, P = .019) and the TS group (161.4 vs 237.1 N, P = .009). Group differences in stiffness did not reach statistical significance among the 3 groups (PL, 33.72 N/mm; BT, 37.50 N/mm; TS, 43.00 N/mm).Conclusion:The TS and BT groups have more ideal biomechanical properties than the PL group, as demonstrated by less displacement during cyclic loading and a higher load to failure.Clinical Relevance:Fixation of the patellar limbs during MPFL reconstruction may be optimized with the use of TS or BT over a PL technique.

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“…2). Moreover, the presence of a crimp period in paraffin‐embedded tissue sections was also confirmed by using hematoxylin/eosin staining, analyzing the tendon sections by light microscopy 6,44 , and using van Gieson staining 45 . Furthermore, Franchi et al 46 .…”

Section: Introductionmentioning

confidence: 92%

Picrosirius‐Polarization Method for Collagen Fiber Detection in Tendons: A Mini‐Review

Liu

et al. 2021

Orthopaedic Surgery

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Although the structure and composition of collagen have been studied by polarized light microscopy since the early 19th century, many studies and reviews have paid little or no attention to the morphological problems of histopathological diagnosis. The morphology of collagen fibers is critical in guiding mechanical and biological properties in both normal and pathological tendons. Highlighting the organization and spatial distribution of tendon‐containing collagen fibers can be very useful for visualizing a tendon's ultrastructure, biochemical and indirect mechanical properties, which benefits other researchers and clinicians. Picrosirius red (PSR) staining, relying on the birefringence of collagen fibers, is one of the best understood histochemical methods that can highly and specifically underline fibers better than other common staining techniques when combined with polarized light microscopy (PLM). Polarized light microscopy provides complementary information about collagen fibers, such as orientation, type and spatial distribution, which is important for a comprehensive assessment of collagen alteration in a tendon. Here, this brief review serves as a simplistic and important primer to research developments in which differential staining of collagen types by the Picrosirius‐polarization method is increasing in diverse studies of the medical field, mainly in the assessment of the morphology, spatial distribution, and content of collagen in tendons.

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Biomechanical and histological comparison between the cryopreserved and the lyophilized Gracilis tendon allograft for MPFL reconstruction, a cadaveric experimental study

Cited by 7 publications

References 20 publications

Dynamic–Anatomical Reconstruction of Medial Patellofemoral Ligament in Open Physis

Dynamic–Anatomical Reconstruction of Medial Patellofemoral Ligament in Open Physis

Cyclic Testing of 3 Medial Patellofemoral Ligament Reconstruction Techniques

Picrosirius‐Polarization Method for Collagen Fiber Detection in Tendons: A Mini‐Review

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