Biomechanical behaviour of human bile duct wall and impact of cadaveric preservation processes.

Girard, Édouard; Chagnon, Grégory; Gremen, Emeric; Calvez, Maxime; Masri, Christopher; Boutonnât, Jean; Trilling, Bertrand; Nottelet, Benjamin

doi:10.1016/j.jmbbm.2019.07.001

Cited by 17 publications

(25 citation statements)

References 39 publications

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“…Some further considerations, in terms of results presented, include that the human tissue obtained for testing was extracted from an embalmed cadaver, rather than a fresh cadaver, due to the restrictions placed on postmortem testing during the COVID-19 pandemic. The cadaver was preserved in formalin after death which could affect the results, as embalming has been found to influence the stiffness of soft tissues when compared to their fresh or frozen counterparts [44,45]. Embalming is considered to most notably affect the collagen of soft tissues, wherein the process partially denatures the fibres, causing an overall softening of the material [44].…”

Section: Considerationsmentioning

confidence: 99%

“…However, formalin solution has also been found to cause collagen cross-links to form [46], which may cause an increase to the overall tissue stiffness. Girard et al [44] found that embalming decreased the mechanical properties of the human bile duct by 80% in the longitudinal direction (more collagen-dense direction) and 40% in the circumferential direction compared to the fresh equivalents, while Hohmann et al [45] found the Young's modulus of the human upper biceps tendon to be approximately 20 times higher for the embalmed tendon compared to the fresh. The effect of embalming as a preservation process within literature has been found to cause mechanical properties that differ from their fresh counterparts, however it is still highly inconclusive in what way.…”

Section: Considerationsmentioning

confidence: 99%

“…The cadaver was preserved in formalin after death which could affect the results, as embalming has been found to influence the stiffness of soft tissues when compared to their fresh or frozen counterparts [44,45]. Embalming is considered to most notably affect the collagen of soft tissues, wherein the process partially denatures the fibres, causing an overall softening of the material [44]. However, formalin solution has also been found to cause collagen cross-links to form [46], which may cause an increase to the overall tissue stiffness.…”

Section: Considerationsmentioning

confidence: 99%

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Experimental investigations of the human oesophagus: anisotropic properties of the muscular layer in large deformation

Durcan¹,

Hossain²,

Chagnon³

et al. 2021

Preprint

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Technological advancements in the field of robotics have led to endoscopic biopsy devices able to extract diseased tissue from between the layers of the gastrointestinal tract. Despite this, the layer-dependent properties of these tissues have yet to be mechanically characterised using human tissue. In this study, the ex vivo mechanical properties of the passive muscularis propia layer of the human oesophagus were extensively investigated. For this, a series of uniaxial tensile tests were conducted. The results displayed hyperelastic behaviour, while the differences between loading the tissue in both the longitudinal and circumferential directions showcased its anisotropy. The anisotropy of the muscular layer was present at different strain rates, with the longitudinal direction being consistently stiffer than the circumferential one. The circumferential direction was found to have little strain-rate dependency, while the longitudinal direction results suggest pronounced strain-rate-dependent behaviour. The repeated trials showed larger variation in terms of stress for a given strain in the longitudinal direction compared to the circumferential direction. The possible causes of variation between trials are discussed, and the experimental findings are linked to the histological analysis which was carried out via various staining methods. Finally, the direction-dependent experimental data was simulated using an anisotropic, hyperelastic model.

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Section: Considerationsmentioning

confidence: 99%

Section: Considerationsmentioning

confidence: 99%

Section: Considerationsmentioning

confidence: 99%

See 1 more Smart Citation

Experimental investigations of the human oesophagus: anisotropic properties of the muscular layer in large deformation

Durcan¹,

Hossain²,

Chagnon³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…There are many similarities between human biliary tract and that of other animals (Li et al 2013). According to the stress-strain curves for the CBD and the BD wall of porcine, dog and human (Jian and Wang 1991;Duch et al 2004;Girard et al 2019) and the effect of aging (Sherratt 2009), the minimum and maximum amount of Ew are selected to be 0.1 MPa and 4 MPa respectively. The maximum and minimum values for Em are based on the elastic moduli and the stress-strain curves of the adjacent organs, body fat, and muscle (Rosen et al 2008, Sparks et al 2015.…”

Section: Combinationsmentioning

confidence: 99%

Numerical Investigation of Bile Secretion and Pressure Rise in Obstructed Human Common Bile Duct

2021

JAFM

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A fully obstructed Common Bile Duct (CBD) could lead to severe implications such as jaundice, cholangitis, and pancreatitis. A 2-D CFD model with the employment of Fluid-structure Interaction (FSI) formulations is established to investigate the interactions of bile with its surroundings. Ascertaining bile secretion against a total CBD obstruction is a major interest of this study. Therefore, a function is assigned to bile secretion pattern such that the resulting intraluminal pressure complies with clinical data. To cover the variation in the parameters representing the mechanical properties of the biliary system as well as bile secretion, specific piecewise ranges are given for each of them and consequently, numerous cases are simulated. Models which after simulation lead to pressure rises in the interval of 700 Pa to 1300 Pa are picked. This interval could roughly include the actual pressure rise of a vast majority of patients. It is determined that among numerous cases, higher distention does not necessarily correlate with higher pressure increase. Furthermore, the effect of alteration in each parameter in pressure rise is determined. This model is the first numerical step towards understanding the pathogenesis of complications resulting from a fully obstructed CBD and deformation of the CBD in general.

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“…Overall, the mechanical tests showed that the viscoelastic properties of the material were stable during the first 8 weeks, whereas the stiffness of the material decreased by approximately 70% after 4 weeks. Despite this rapid decrease in the degree of stiffness, the PCL-TIB/PLA50-b-PEG-b-PLA50 blend can be further considered for RIBS as its initial mechanical properties are approximately10 times higher than those of biliary duct [39,60]. This should ensure that the mechanical properties of the RIBS remain high enough after 4 weeks to avoid anastomotic stricture and hence keep the tube lumen open, thus preventing biliary obstruction.…”

Section: Evolution Of the Mechanical Propertiesmentioning

confidence: 99%

From in vitro evaluation to human postmortem pre-validation of a radiopaque and resorbable internal biliary stent for liver transplantation applications

et al. 2020

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The implantation of an internal biliary stent (IBS) during liver transplantation has recently been shown to reduce biliary complications. To avoid a potentially morbid ablation procedure, we developed a resorbable and radiopaque internal biliary stent (RIBS). We studied the mechanical and radiological properties of RIBS upon in vivo implantation in rats and we evaluated RIBS implantability in human anatomical specimens.For this purpose, a blend of PLA50-PEG-PLA50 triblock copolymer, used as a polymer matrix, and of X-ray-visible triiodobenzoate-poly(-caprolactone) copolymer (PCL-TIB), as a radiopaque additive, was used to design X-ray-visible RIBS. Samples were implanted in the peritoneal cavity of rats. The radiological, chemical, and biomechanical properties were evaluated during degradation. Further histological studies were carried out to evaluate the degradation and compatibility of the RIBS. A human cadaver implantability study was also performed.The in vivo results revealed a decline in the RIBS mechanical properties within 3 months, whereas clear and stable X-ray visualization of the RIBS was possible for up to 6 months. Histological analyses confirmed compatibility and resorption of the RIBS, with a limited inflammatory response. The RIBS could be successfully implanted in human anatomic specimens. The results reported in this study will allow the development of trackable and degradable IBS to reduce biliary complications after liver transplantation.

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Biomechanical behaviour of human bile duct wall and impact of cadaveric preservation processes.

Cited by 17 publications

References 39 publications

Experimental investigations of the human oesophagus: anisotropic properties of the muscular layer in large deformation

Experimental investigations of the human oesophagus: anisotropic properties of the muscular layer in large deformation

Numerical Investigation of Bile Secretion and Pressure Rise in Obstructed Human Common Bile Duct

From in vitro evaluation to human postmortem pre-validation of a radiopaque and resorbable internal biliary stent for liver transplantation applications

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