Biomechanical evaluation of tendon regeneration with adipose‐derived stem cell

Santos, Alex de Lima; Silva, Camila Gonzaga da; Barretto, Letícia Siqueira de Sá; Franciozi, Carlos Eduardo da Silveira; Tamaoki, Marcel Jun Sugawara; Almeida, Fernando G.; Faloppa, Flávio

doi:10.1002/jor.24182

Cited by 23 publications

(13 citation statements)

References 22 publications

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“…Indeed, they are able to differentiate not only into cells of mesodermal origin such as adipocytes [ 1 , 2 ], chondrocytes [ 3 ] and osteocytes [ 4 ], but also into cells of different lineages [ 5 ]. For this reason, they have been extensively investigated for potential therapeutic applications in a variety of pathologies, being also capable of releasing immunosuppressive cytokines such as interleukin-10 (IL-10), or factors promoting tissue regeneration (hepatocyte growth factor, and transforming growth factor-β; TGF-β) [ 6 , 7 , 8 , 9 ]. Stem-cell-based therapies are currently explored for the treatment of ocular diseases [ 10 , 11 , 12 , 13 ], including diabetic retinopathy (DR), in which a vascular complication leads to the disruption of the blood retinal barrier (BRB) [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of High Glucose Concentration on Pericyte-Like Differentiated Human Adipose-Derived Mesenchymal Stem Cells

Mannino

Longo

Gennuso

et al. 2021

IJMS

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A pericyte-like differentiation of human adipose-derived mesenchymal stem cells (ASCs) was tested in in vitro experiments for possible therapeutic applications in cases of diabetic retinopathy (DR) to replace irreversibly lost pericytes. For this purpose, pericyte-like ASCs were obtained after their growth in a specific pericyte medium. They were then cultured in high glucose conditions to mimic the altered microenvironment of a diabetic eye. Several parameters were monitored, especially those particularly affected by disease progression: cell proliferation, viability and migration ability; reactive oxygen species (ROS) production; inflammation-related cytokines and angiogenic factors. Overall, encouraging results were obtained. In fact, even after glucose addition, ASCs pre-cultured in the pericyte medium (pmASCs) showed high proliferation rate, viability and migration ability. A considerable increase in mRNA expression levels of the anti-inflammatory cytokines transforming growth factor-β1 (TGF-β1) and interleukin-10 (IL-10) was observed, associated with reduction in ROS production, and mRNA expression of pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), and angiogenic factors. Finally, a pmASC-induced better organization of tube-like formation by retinal endothelial cells was observed in three-dimensional co-culture. The pericyte-like ASCs obtained in these experiments represent a valuable tool for the treatment of retinal damages occurring in diabetic patients.

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Section: Introductionmentioning

confidence: 99%

Effects of High Glucose Concentration on Pericyte-Like Differentiated Human Adipose-Derived Mesenchymal Stem Cells

Mannino

Longo

Gennuso

et al. 2021

IJMS

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“…Спустя 4 недели выявлено, что сухожилия имеют большую площадь сечения, меньшую жёсткость и меньшую предельную растягивающую нагрузку в сравнении с животными, которым МСК не вводили. В данном эксперименте пришли к выводу, что МСК успешно участвуют в дифференцировке теноцитов на ранних стадиях регенерации и вносят значительный вклад в дальнейшую реконструкцию сухожилий [8]. Однако увеличение площади поперечного сечения может ухудшить движение сухожилия впоследствии, а также в данном эксперименте не проведены гистологическая или функциональная оценки, что подразумевает дальнейшие исследования.…”

Section: применение мск для восстановления сухожилий в экспериментеunclassified

“…Таким образом, тканевая инженерия, основанная на репаративном потенциале мезенхимальных стволовых клеток, доставляемых к месту повреждения сухожилия самостоятельно или в сочетании с биосовместимыми конструкциями или гелями, в зависимости от типа повреждения и стадии заболевания является наиболее перспективным направлением регенеративной медицины [8,63,64]. Однако отсутствие протоколов, а также неблагоприятные результаты некоторых исследований предполагают дальнейшее изучение применения мезенхимальных стволовых клеток в регенерации повреждённых сухожилий.…”

Section: заключениеunclassified

“…Вместо этого заживление происходит с образованием рубца, что ставит под угрозу дальнейшую механическую и структурную функциональность сухожилия [6]. Из-за гиповаскулярной природы заживление происходит медленно и часто не полностью, поэтому риск повторного разрыва сухожилия или связки остаётся высоким [7,8,9]. Перспективным направлением в регенерации повреждённых сухожилий является применение клеточных технологий.…”

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Cellular Technologies in Traumatology: From Cells to Tissue Engineering

Дремина

Trukhan

Shurygina

2021

Acta biomedica scientifica

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Injuries and degenerative changes of tendons are common damages of the musculoskeletal system. Due to its hypovascular character the tendon has a limited natural ability to recover. For typical surgical treatment, the tendon integrity is restored, but in most cases, there occurs formation of the connective tissue scar resulting in structural and mechanical functionality disruption. The insufficient effectiveness of traditional therapy methods requires the search for alternative ways to restore damaged tendon tissues. This article discusses new effective methods for improving the treatment that base on the use of cellular technologies among which one of the main directions is mesenchymal stem cell application. Due to mesenchymal stem cells, there is a shift from pro-fibrotic and pro-inflammatory reactions of cells to pro-regenerative ones. Stem cells being multipotent and having among other things tenogenic potential are considered a promising material for repairing damaged tendons. The article also describes the sources of progenitor tendon cells including the tendon bundles and pericytes the main markers of which are Scx and Mkx that are proteins of the transcription factor superfamily, and Tnmd that is transmembrane glycoprotein.The growth factors that not only enhance the proliferative activity of mesenchymal stem cells but also promote in vitro tenogenic genes expression as well as the collagen Itype production what is necessary for tendon formation are considered. Along with growth factors, the morphogenetic protein BMP14 is presented, this protein increases themesenchymal stem cell proliferation and contributes directed tenogenic differentiation of these cells, suppressing their adipogenic and chondrogenic potentials.In recent years, mesenchymal stem cells have been used both separately and in combination with various growth factors and different three-dimensional structures providing the interaction with all of the cell types.The issues of the latest 3D-bioprinting technology allowing to make tissue-like structures for replacement damaged tissues and organs are discussed. 3D-bioprinting technology is known to allow acting exact spatio-temporal control of the distribution of cells, growth factors, small molecules, drugs and biologically active substances.

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“…To determine if ASCs can augment tendon healing, de Lima Santos et al delivered ASCs with suture repair of a rabbit flexor tendon injury. This study showed that ASC delivery significantly improved biomechanical properties of tendon repair as compared with acellular groups.…”

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confidence: 99%