Biomechanical Remodeling of Biodegradable Small-Diameter Vascular Grafts in Situ

Глушкова, Т. В.; Sevost'yanova, V. V.; Антонова, Л. В.; Klyshnikov, K. Yu.; Овчаренко, Е. А.; Sergeeva, Evgeniya A.; Vasyukov, G. Yu.; Seifalian, Alexander M.; Ls, Barbarash

doi:10.15825/1995-1191-2016-2-99-109

Cited by 6 publications

(7 citation statements)

References 11 publications

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“…In addition, the sections of the PHBV/ PCL/VEGF, PHBV/PCL/bFGF, and PHBV/PCL/SDF1a grafts with the diameter of 2 mm, which long-term patency has been reported previously in [3,5], and the sections of the non-woven PHBV/PCL/VEGF, PHBV/ PCL/bFGF, PHBV/PCL/SDF-1a matrices, the respond to their subcutaneous implantation has been studied in [6], underwent the similar staining procedure for the assessment. They were then immersed in a 2% aqueous solution of alizarin red C for 70 seconds, then nuclei of cells were stained with DAPI 10 μg/ml for 3 minutes.…”

Section: Calcification Assessmentmentioning

confidence: 56%

In Situ Vascular Tissue Remodeling Using Biodegradable Tubular Scaffolds With Incorporated Growth Factors and Chemoattractant Molecules

Антонова

Sevost'yanova

Миронов

et al. 2018

Kompleks. probl. serdečno-sosud. zabol.

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25омплексные проблемы сердечно-сосудистых заболеваний К Highlights • The study demonstrates the benefits of using functionalized small-diameter biodegradable vascular graft for de novo vascular tissue formation.• The release of growth factors and chemoattractant molecules into the vascular bed after the graft implantation stimulates neotissue formation and levels out inflammation and calcification. BackgroundCurrently, the search for the bioactive molecules capable of promoting formation of the vascular tissue is still ongoing. We have previously demonstrated that incorporation of the growth factors and chemoattractant molecules into the biodegradable tubular scaffolds can increase their primary patency upon the implantation into rat abdominal aorta. However, further studies are required to investigate tissue remodeling using functionalized vascular grafts with the same diameter as a replaced native vessel. AimTo investigate the specific aspects of de novo vascular tissue formation and calcification employing rat abdominal aorta interposition model and vascular grafts with 1.5 mm diameter with incorporated vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and stromal cell-derived factor (SDF)-1α. MethodsTubular grafts with a diameter of 1.5 mm were blended of poly(3-hydroxybutyrateco-3-hydroxyvalerate) and poly(ε-caprolactone) (PHBV/PCL). Grafts without growth factors were fabricated using standard electrospinning technique whilst grafts with incorporated growth factors were prepared utilizing emulsion electrospinning. VEGF was incorporated into the inner third, whereas bFGF and SDF-1α were incorporated into the outer two-thirds of the graft. Grafts were implanted into the abdominal aortas of Wistar rats for 1, 3, 6, and 12 months following scanning electron microscopy along with histological and immunofluorescent examination. ResultsPrimary patency of the grafts with VEGF, bFGF, and SDF-1α reached 93% indicative of structural integrity of the vascular tissue. Neither signs of inflammation nor severe calcification was detected. ConclusionAs in 2 mm diameter vascular grafts, incorporation of bioactive factors into 1.5 mm diameter grafts increased their long-term primary patency and improved vascular tissue formation in comparison with non-modified grafts.

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Section: Calcification Assessmentmentioning

confidence: 56%

In Situ Vascular Tissue Remodeling Using Biodegradable Tubular Scaffolds With Incorporated Growth Factors and Chemoattractant Molecules

Антонова

Sevost'yanova

Миронов

et al. 2018

Kompleks. probl. serdečno-sosud. zabol.

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“…Previously, we showed that PHBV/PCL vascular grafts are populated by ECM-producing cells within 6 months following implantation, which results in a decrease in their relative elongation and Young’s modulus. 30 …”

Section: Discussionmentioning

confidence: 99%

Biodegradable Patches for Arterial Reconstruction Modified with RGD Peptides: Results of an Experimental Study

et al. 2020

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Modification by Arg-Gly-Asp (RGD) peptides is a promising approach to improve the biocompatibility of biodegradable vascular patches for arteriotomy. In this study, we evaluated the performance of vascular patches electrospun using a blend of polycaprolactone (PCL) and polyhydroxybutyrate/valerate (PHBV) and additionally modified with RGDK, AhRGD, and c[RGDFK] peptides using 1,6-hexamethylenediamine or 4,7,10-trioxa-1,13-tridecanediamine (TTDDA) linkers. We examined mechanical properties and hemocompatibility of resulting patches before implanting them in rat abdominal aortas to assess their performance in vivo. Patches were explanted 1, 3, 6, and 12 months postoperation followed by histological and immunofluorescence analyses. Patches manufactured from the human internal mammary artery or commercially available KemPeriplas-Neo xenopericardial patches were used as a control. The tensile strength and F max of KemPeriplas-Neo patches were 4- and 16.7-times higher than those made of human internal mammary artery, respectively. Both RGD-modified and unmodified PHBV/PCL patches demonstrated properties similar to a human internal mammary artery patch. Regardless of RGD modification, experimental PHBV/PCL patches displayed fewer lysed red blood cells and resulted in milder platelet aggregation than KemPeriplas-Neo patches. Xenopericardial patches failed to form an endothelial layer in vivo and were prone to calcification. By contrast, TTDDA/RGDK-modified biodegradable patches demonstrated a resistance to calcification. Modification by TTDDA/RGDK and TTDDA/c[RGDFK] facilitated the formation of neovasculature upon the implantation in vivo.

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“…Так, ранее мы показали изменение механических свойств пористого биодеградируемого материала в соответствии со свойствами нативных тканей после 6 мес. имплантации in vivo [27].…”

Section: Discussionunclassified

Tissue-engineered patch modified by vascular endothelial growth factor for reconstruction of vascular wall

Sevost'yanova¹,

Миронов²,

Антонова³

et al. 2020

PKiK

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Background. Commercially available synthetic and animal-derived vascular patches used in patch angioplasty during carotid endarterectomy have several disadvantages, such as postoperative thrombosis or occlusion and restenosis. This problem may be resolved by the development of biologically active materials that are biodegradable and can stimulate tissue regeneration. Aim. To evaluate the properties and efficacy of a biodegradable patch based on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(ε-caprolactone) (PCL) into which vascular endothelial growth factor (VEGF) is incorporated, in comparison with unmodified PHBV/PCL and commercial vascular patches.Methods. Porous patches were fabricated by emulsion electrospinning from a mixture of PHBV and PCL, into which VEGF was incorporated. The morphological and mechanical properties of these patches were tested, and they were implanted into the wall of rat abdominal aortas for 1, 3, 6 and 12 months. Histological and immunofluorescence examinations were performed to evaluate endothelisation, cellular composition and calcification.Results. PHBV / PCL patches with VEGF had a highly porous structure and demonstrated tensile strength similar to that of the aorta in rats and the internal thoracic artery in humans. After 3 months of implantation, an endothelial monolayer was formed on the inner surface of these patches. The patches were populated by cells that secreted the extracellular matrix faster than did cells of patches from the xenopericardium. Remodelling with PHBV / PCL patches was not accompanied by chronic inflammation; in contrast, inflammation was observed with long-term implantation of unmodified PHBV / PCL samples.Conclusion. VEGF incorporated into biodegradable PHBV / PCL patches stimulated their endothelisation, increased their biocompatibility and promoted remodelling and formation of the components of the blood vessel. PHBV / PCL / VEGF patches thus have a high potential for use in tissue engineering of the vascular wall.Received 2 June 2020. Revised 27 June 2020. Accepted 16 July 2020.Funding: This study was supported by the Complex Program of Basic Research under the Siberian Branch of the Russian Academy of Sciences within the Basic Research Topic of Research Institute for Complex Issues of Cardiovascular Diseases № 0546-2019-0002 “Pathogenetic basis for the development of cardiovascular implants from biocompatible materials using patient-oriented approach, mathematical modeling, tissue engineering, and genomic predictors”.Conflict of interest: Authors declare no conflict of interest.Author contributions Conception and study design: V.V. Sevostianova, A.V. Mironov, L.V. Antonova, R.S. Tarasov, L.S. Barbarash Data collection and analysis: V.V. Sevostianova, A.V. Mironov, L.V. Antonova, E.O. Krivkina, V.G. Matveeva, E.A. Velikanova, T.V. Glushkova Statistical analysis: V.V. Sevostianova, T.V. Glushkova Drafting the article: V.V. Sevostianova, A.V. Mironov Critical revision of the article: L.V. Antonova, R.S. Tarasov, L.S. Barbarash Final approval of the version to be published: V.V. Sevostianova, A.V. Mironov, L.V. Antonova, E.O. Krivkina, V.G. Matveeva, E.A. Velikanova, R.S. Tarasov, T.V. Glushkova, L.S. Barbarash

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Biomechanical Remodeling of Biodegradable Small-Diameter Vascular Grafts in Situ

Cited by 6 publications

References 11 publications

In Situ Vascular Tissue Remodeling Using Biodegradable Tubular Scaffolds With Incorporated Growth Factors and Chemoattractant Molecules

In Situ Vascular Tissue Remodeling Using Biodegradable Tubular Scaffolds With Incorporated Growth Factors and Chemoattractant Molecules

Biodegradable Patches for Arterial Reconstruction Modified with RGD Peptides: Results of an Experimental Study

Tissue-engineered patch modified by vascular endothelial growth factor for reconstruction of vascular wall

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