Purpose Six different mono-axial and poly-axial distal humeral plating systems with an anatomical plate design were compared. The aim of the biomechanical tests was to examine differences regarding system stiffness, median fatigue limit, and failure mechanisms. Methods Different configurations of two double plate fixation systems by two manufacturers for the treatment of complex distal humeral fractures (AO/OTA type C2.3) were biomechanically tested in a physiologically relevant setup. Results The 180°Stryker configuration presented itself as the system with the highest stiffness, being significantly stiffer (p < 0.001) than every system other than the poly-axial 180°aap system (p = 0.378). For the median fatigue limit the 180°S tryker and poly-axial aap systems were ranked first and second. The failure mechanism for all 90°systems was a fatigue breakage of the posterolateral plate. The 180°aap systems demonstrated breakage of the most distal screws of the lateral plate. The 180°Stryker system demonstrated screw breakage on both the medial and lateral plates. Discussion Breakage of the posterolateral plate as a failure mechanism for the 90°systems was expected. The 180°s ystems demonstrated a higher stiffness compared to the 90°c onstructs for the axial loading. In conclusion, both poly-axial anatomical plating systems provide sufficient stability in this scenario, and the 180°configurations demonstrated superior stiffness.