Biomechanically, structurally and functionally meticulously tailored polycaprolactone/silk fibroin scaffold for meniscus regeneration

Zong, Li; Wu, Nier; Cheng, Jin; Sun, Muyang; Yang, Peng; Zhao, Fengyuan; Zhang, Jiahao; Duan, Xin; Fu, Xin; Zhang, Jiying; Hu, Xiaoqing; Chen, Haifeng; Ao, Yingfang

doi:10.7150/thno.44270

Cited by 86 publications

(91 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Scaffolds play a crucial role in regenerative medicine by providing the appropriate 3D environment for cell adhesion, proliferation, and matrix production 40 , 41 . An ideal scaffold should, therefore, be similar in function and structure to normal tissue and support the differentiation and formation of different tissue morphologies.…”

Section: Discussionmentioning

confidence: 99%

Preparation of high precision multilayer scaffolds based on Melt Electro-Writing to repair cartilage injury

Han¹,

Lian²,

Sun³

et al. 2020

Theranostics

View full text Add to dashboard Cite

Rationale: Articular cartilage injury is quite common. However, post-injury cartilage repair is challenging and often requires medical intervention, which can be aided by 3D printed tissue engineering scaffolds. Specifically, the high accuracy of Melt Electro-Writing (MEW) technology facilitates the printing of scaffolds that imitate the structure and composition of natural cartilage to promote repair. Methods: MEW and Inkjet printing technology was employed to manufacture a composite scaffold that was then implanted into a cartilage injury site through microfracture surgery. While printing polycaprolactone (PCL) or PCL/hydroxyapatite (HA) scaffolds, cytokine-containing microspheres were sprayed alternately to form multiple layers containing transforming growth factor-β1 and bone morphogenetic protein-7 (surface layer), insulin-like growth factor-1 (middle layer), and HA (deep layer). Results: The composite biological scaffold was conducive to adhesion, proliferation, and differentiation of mesenchymal stem cells recruited from the bone marrow and blood. Meanwhile, the environmental differences between the scaffold's layers contributed to the regional heterogeneity of chondrocytes and secreted proteins to promote functional cartilage regeneration. The biological effect of the composite scaffold was validated both in vitro and in vivo . Conclusion: A cartilage repair scaffold was established with high precision as well as promising mechanical and biological properties. This scaffold can promote the repair of cartilage injury by using, and inducing the differentiation and expression of, autologous bone marrow mesenchymal stem cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Preparation of high precision multilayer scaffolds based on Melt Electro-Writing to repair cartilage injury

Han¹,

Lian²,

Sun³

et al. 2020

Theranostics

View full text Add to dashboard Cite

show abstract

“…Moreover, it has been proved that CD146 + subpopulation from adipose-derived mesenchymal stem cells (ADSCs) play essential roles in promoting cartilage repair 168 . And composite scaffold combined with synovium-derived mesenchymal stem cell (SMSC) could greatly strengthen chondroprotection 170 . Therefore, MSCs have been considered as the most promising cells in cartilage tissue engineering in recent years, methods to improve the chondrogenesis of which are feasible and could potentially be achieved using injectable hydrogels, which would provide them with strong chondrogenic cues and prevent their differentiation toward unwanted tissues.…”

Section: Design Of Injectable Hydrogelsmentioning

confidence: 99%

Exquisite design of injectable Hydrogels in Cartilage Repair

Chen

Deng

et al. 2020

Theranostics

View full text Add to dashboard Cite

Cartilage damage is still a threat to human beings, yet there is currently no treatment available to fully restore the function of cartilage. Recently, due to their unique structures and properties, injectable hydrogels have been widely studied and have exhibited high potential for applications in therapeutic areas, especially in cartilage repair. In this review, we briefly introduce the properties of cartilage, some articular cartilage injuries, and now available treatment strategies. Afterwards, we propose the functional and fundamental requirements of injectable hydrogels in cartilage tissue engineering, as well as the main advantages of injectable hydrogels as a therapy for cartilage damage, including strong plasticity and excellent biocompatibility. Moreover, we comprehensively summarize the polymers, cells, and bioactive molecules regularly used in the fabrication of injectable hydrogels, with two kinds of gelation, i.e., physical and chemical crosslinking, which ensure the excellent design of injectable hydrogels for cartilage repair. We also include novel hybrid injectable hydrogels combined with nanoparticles. Finally, we conclude with the advances of this clinical application and the challenges of injectable hydrogels used in cartilage repair.

show abstract

“…Synthetic polymers are human-made materials that are already widely used in cartilage tissue engineering for their well-characterized and stable chemical properties. Polymers such as poly(ethylene)-glycol (PEG), polycaprolactone (PCL), or polyglycolic acid (PGA) can be combined or coated with hydrogels or natural polymers to enhance their biocompatibility [ 91 , 92 , 93 , 94 ]. Another class of material developing is nanomaterials, such as carbon nanotubes (CNTs) for their physico-chemical properties [ 95 ].…”

Section: Bioextrusion Processes For Cartilage Tissue Engineeringmentioning

confidence: 99%

Stem Cells and Extrusion 3D Printing for Hyaline Cartilage Engineering

Messaoudi

Henrionnet

Bourge

et al. 2020

Cells

View full text Add to dashboard Cite

Hyaline cartilage is deficient in self-healing properties. The early treatment of focal cartilage lesions is a public health challenge to prevent long-term degradation and the occurrence of osteoarthritis. Cartilage tissue engineering represents a promising alternative to the current insufficient surgical solutions. 3D printing is a thriving technology and offers new possibilities for personalized regenerative medicine. Extrusion-based processes permit the deposition of cell-seeded bioinks, in a layer-by-layer manner, allowing mimicry of the native zonal organization of hyaline cartilage. Mesenchymal stem cells (MSCs) are a promising cell source for cartilage tissue engineering. Originally isolated from bone marrow, they can now be derived from many different cell sources (e.g., synovium, dental pulp, Wharton’s jelly). Their proliferation and differentiation potential are well characterized, and they possess good chondrogenic potential, making them appropriate candidates for cartilage reconstruction. This review summarizes the different sources, origins, and densities of MSCs used in extrusion-based bioprinting (EBB) processes, as alternatives to chondrocytes. The different bioink constituents and their advantages for producing substitutes mimicking healthy hyaline cartilage is also discussed.

show abstract

Biomechanically, structurally and functionally meticulously tailored polycaprolactone/silk fibroin scaffold for meniscus regeneration

Cited by 86 publications

References 41 publications

Preparation of high precision multilayer scaffolds based on Melt Electro-Writing to repair cartilage injury

Preparation of high precision multilayer scaffolds based on Melt Electro-Writing to repair cartilage injury

Exquisite design of injectable Hydrogels in Cartilage Repair

Stem Cells and Extrusion 3D Printing for Hyaline Cartilage Engineering

Contact Info

Product

Resources

About