Biomechanische Merkmale im Fokus Hybrid- und Verbundwerkstoffe mit schmelzähnlichen Eigenschaften

Kern, Manfred

doi:10.1055/s-0035-1559761

Cited by 2 publications

(1 citation statement)

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“…They possess varying biochemical and biomechanical properties, thus creating a plethora of biological interfaces [ 16 ]. This is, e.g., reflected by the different extracellular matrix (ECM) composition as well as stiffnesses/elasticities of the hard and soft tissues within the dental pulp [ 17 ]. These facts lead to several as-yet unsolved problems in dental pulp regeneration: (i) the selection of the proper cell type for tissue engineering approaches, (ii) induction and in situ maintenance of cell differentiation and function, and (iii) the biochemical and biomechanical design of biomaterials aiming at optimally supporting dental pulp regeneration.…”

Section: Introductionmentioning

confidence: 99%

Biomechanical Modulation of Dental Pulp Stem Cell (DPSC) Properties for Soft Tissue Engineering

et al. 2023

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Dental pulp regeneration strategies frequently result in hard tissue formation and pulp obliteration. The aim of this study was to investigate whether dental pulp stem cells (DPSCs) can be directed toward soft tissue differentiation by extracellular elasticity. STRO-1-positive human dental pulp cells were magnetically enriched and cultured on substrates with elasticities of 1.5, 15, and 28 kPa. The morphology of DPSCs was assessed visually. Proteins relevant in mechanobiology ACTB, ITGB1, FAK, p-FAK, TALIN, VINCULIN, PAXILLIN, ERK 1/2, and p-ERK 1/2 were detected by immunofluorescence imaging. Transcription of the pulp marker genes BMP2, BMP4, MMP2, MMP3, MMP13, FN1, and IGF2 as well as the cytokines ANGPT1, VEGF, CCL2, TGFB1, IL2, ANG, and CSF1 was determined using qPCR. A low stiffness, i.e., 1.5 kPa, resulted in a soft tissue-like phenotype and gene expression, whereas DPSCs on 28 kPa substrates exhibited a differentiation signature resembling hard tissues with a low cytokine expression. Conversely, the highest cytokine expression was observed in cells cultured on intermediate elasticity, i.e., 15 kPa, substrates possibly allowing the cells to act as “trophic mediators”. Our observations highlight the impact of biophysical cues for DPSC fate and enable the design of scaffold materials for clinical pulp regeneration that prevent hard tissue formation.

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Section: Introductionmentioning

confidence: 99%