Biomedical application of Mössbauer spectroscopy with a high velocity resolution: Revealing of small variations

Abstract

Application of Mössbauer spectroscopy with a high velocity resolution for study different hemoglobins, ferritin, its models and chicken liver and spleen as well as normal and lymphoid chicken spleen demonstrated revealing of small variations of hyperfine parameters related to small variations of iron stereochemistry in biomolecules. These data demonstrate that Mössbauer spectroscopy with a high velocity resolution may be useful in biomedical research to distinguish small variations of iron-containing proteins in normal and pathological cases.

Mössbauer spectroscopy of the iron cores in human liver ferritin, ferritin in normal human spleen and ferritin in spleen from patient with primary myelofibrosis: preliminary results of comparative analysis

“…Therefore, it is interesting to test the iron overload in spleen tissue at this pathology and compare with known secondary iron overload spleen data. In this study we applied Mössbauer spectroscopy with a high velocity resolution for measurements at 295 and 90 K and conventional Mössbauer spectroscopy for measurements at 20 K. It should be noted that Mössbauer spectroscopy with a high velocity resolution demonstrated more possibilities for detailed analysis of studied biological materials (see Oshtrakh et al 2010Oshtrakh et al , 2011bOshtrakh et al , 2011cOshtrakh and Semionkin 2013).…”

Mössbauer spectroscopy with a high velocity resolution: Advances in biomedical, pharmaceutical, cosmochemical and nanotechnological research

“…The first results of the application of Mössbauer spectroscopy with a high velocity resolution in biomedical research were recently considered in [11][12][13][14][15][16]. One of the main results obtained in the study of iron storage protein ferritin extracted from human liver demonstrated that an increase in statistical counting rate in the spectrum cannot compensate an increase in velocity resolution (in the number of spectral points) [17].…”

The 57Fe hyperfine interactions in the life sciences: application of Mössbauer spectroscopy with a high velocity resolution in the study of iron-containing biomolecules and pharmaceutical compounds

“…Since 2006 we are developing the high velocity resolution Mössbauer spectroscopy which significantly increases the analytical possibilities of the technique with decreasing the instrumental (systematic) error in evaluation of the 57 Fe hyperfine parameters and the most reliable fitting of complicated Mössbauer spectra. The first results of the biomedical applications of the latter method was considered in [18][19][20][21][22][23]. In this review we discuss recent results of Mössbauer study of different normal tetrameric hemoglobins and monomeric leghemoglobins, human hemoglobin from patients with some hematological diseases, human liver and bacterial ferritin and liver and spleen tissues containing iron storage proteins from normal persons and patients with some hematological diseases, and pharmaceutical products.…”